31 results about "Beta-Hydroxybutyric acid" patented technology

The method for synthesizing completely-degradable plastics poly beta-hydroxybutyrate includes the folloiwng steps: adopting acetaldehyde as raw material to prepare beta-hydroxybutanal; oxidizing it to prepare beta-hydroxybutyric acid; esterifying it to prepare ethyl beta-hydroxybutyrate and polymerizing it to prepare poly beta-hydroxybutyrate. It is characterized by that preparation of beta-hydroxybutanal adopts benzene as solvent, sodium hydroxide as catalyst, its reaction temp. is 1-15 deg.C and the organic acid is used as neutralizing reagent, the preparation of beta-hydroxybutyric acid adopts ethyl acetate as solvent, cobalt acetate as catalyst, its reaction temp. is 40-100 deg.c and reaction pressure is 0.3-1.5 MPa, the preparation of ethyl ebeta-hydroxybutyrate adopts cresulsulfonic acid as catalyst and ethyl alcohol as solvent and polymerization preparation of beta-hydroxybutyrate uses isopropyl titanate as catalyst.

The invention discloses a method for preparing a test paper for rapidly determining dairy cows subclinical ketosis, which comprises the following steps of: adding boric acid, aminoacetic acid and ethylene diamine tetraacetic acid solution in Arabic gum dissolved in a hot-water bath, adjusting the pH value of the prepared solution to be 7.0, soaking the test paper for 20 minutes in the prepared solution while the prepared solution is hot, drying the test paper in a drying oven at 60 DEG C, and standing the test paper in the drying oven overnight; and dissolving ethylene glycol, sodium nitroprusside and absolute ethyl alcohol in the hot-water bath, soaking the test paper for 20 minutes in the prepared solution while the prepared solution is hot, taking the test paper out, blotting moisture in the test paper by using filter paper, drying the test paper in the drying oven at 60 DEG C, and standing the test paper in the drying oven overnight so as to obtain the test paper. The reagent strip takes the concentration of beta-hydroxybutyric acid in blood more than 1.2mmol / L as a positive criteria; and the production practice proves that the reagent strip has the advantages of low cost, simple and easy operation, quick response, high specificity and high sensitivity, can be used for various determined samples such as blood, urine, serum, plasma and milk, and has wide application scope.

The invention provides a method for measuring beta-hydroxybutyric acid by using enzyme colorimetric reaction, a matched kit and application thereof. Under the system of Tris-HCL buffer solution, serum beta-hydroxybutyric acid and coenzyme I are dehydrogenized under the catalysis of beta-hydroxybutyric acid dehydrogenase to generate acetoacetic acid and reduced coenzyme I, the generated reduced coenzyme I and iodonitrotetrazole chloride are reacted under the catalysis of diaphorase to generate a red substance formazane of which the highest absorbance is 505 nanometers, and then the content of the beta-hydroxybutyric acid in a biological sample is quantified by measuring the change of the absorbance of the red product at the wavelength of 505 nanometers. The method can linearly measure the concentration range (0 to 4.5 mmol / L) of the beta-hydroxybutyric acid in the biological sample, the measurement is used for judging the human ketosis for acid poisoning diagnosis, the reagent used by the method is liquid dual-reagent, and the quantity of the sample required for measuring the beta-hydroxybutyric acid is little; moreover, the reaction time during measuring is short, the operation is simple, and the method is suitable for mass detection.