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Kit for detecting urinary lactic acid, creatine and beta-hydroxybutyric acid in human urine simultaneously

A technology for detecting human bodies and kits, applied in biological testing, material inspection products, etc., can solve problems such as semi-quantitative detection, false positives, interference, etc., and achieve the effects of improving stability and accuracy, ensuring stability, and ensuring results

Active Publication Date: 2010-09-08
北京中生金域诊断技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no related inventions or commercial kits aimed at evaluating weight loss effects in China. There are test strips for similar purposes abroad (patent No. US 2004 / 0043376 A1), but only detects one indicator of β-hydroxybutyric acid in human urine. Can not fully reflect the impact of weight loss on human metabolism, and can only be detected qualitatively, not semi-quantitatively
[0006] Keto from GDS Technology, USA It is a dry chemical test strip that can be used to measure the concentration of β-hydroxybutyric acid in blood samples. For the determination of the concentration of β-hydroxybutyric acid in urine, there are the following defects: 1. The pH value must be controlled at 8.5 during the reaction Above, the pH of urine often cannot reach this pH value, which often leads to false positive results. negative result
3. There is no device for purifying urine, and the result is easily interfered by other substances in the urine
[0008] Before measuring the concentration of urinary lactic acid, creatine and β-hydroxybutyric acid, it is necessary to remove the interfering substances in the urine. The foreign products of the same kind use the following methods to remove the interfering substances in the urine: a. Through the dilution of urine (1; 20~1:5 ) to reduce the influence of interfering substances, but the analyte is also diluted at the same time, the accuracy of the measurement results is reduced, and it is not suitable for the determination of low-concentration lactic acid, creatine and β-hydroxybutyric acid
B. Use an ion exchange column such as Ambertile IR-45 (OH-owex 50W×8(H + ) to form an anion and cation exchange column, although the accuracy of the measurement result is improved, but the sample is also diluted while removing the interference, and the operation process is complicated and time-consuming
[0009] Take the method that dilution and ion exchange are combined when interfering substance in liquid of commercial kit Galactosur (Sweden) and GB 1430512 (patent number, Japan): urine 1: 5 or 1: 2 dilution; Chromatographic paper; this treatment can reduce the influence of interfering substances on the reaction to a certain extent, but it cannot completely reduce the concentration of electrolytes in the urine when the subjects ingest a large amount of fruit, or ingest VitC drugs, or other reasons cause excessive electrolyte concentration. Remove interfering substances in urine, so that enzymatic reactions cannot proceed

Method used

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  • Kit for detecting urinary lactic acid, creatine and beta-hydroxybutyric acid in human urine simultaneously
  • Kit for detecting urinary lactic acid, creatine and beta-hydroxybutyric acid in human urine simultaneously
  • Kit for detecting urinary lactic acid, creatine and beta-hydroxybutyric acid in human urine simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Urine Purification Device

[0071] Such as figure 1 As shown, the urine purification device is a titration container, including a filling chamber 2, the top of the filling chamber 2 has a liquid filling port 8, and a sealing cap 1 is provided at the liquid filling port 8. The chamber 2 is filled with a selective adsorbent 3, the bottom of the packing chamber 2 has a liquid outlet 6, the liquid outlet 6 is conical and protruding, and has a plug cap 7 at the protruding end. The bottom of the packing chamber 2 has a clamping membrane ring 4, and a filter membrane 5 is located below the clamping membrane ring 4.

Embodiment 2

[0072] Example 2 Detection Kit

[0073] Urine Purification Device

[0074] The structure of the urine purification device is the same as in Example 1. In this example, the selective specific adsorbent is an adsorbent composed of 0.5g neutral alumina and 0.3g of purified activated carbon, wherein the purified activated carbon is treated with 10% NaOH, and the selective adsorbent is baked at 70°C for 72 hours. activation. The activated adsorbent can completely absorb and remove interfering substances in urine such as bilirubin, vitamin C and other interfering substances.

[0075] Preparation of dry chemical reaction device

[0076] Prepare lactate oxidase pads:

[0077] The 3MM grade chromatographic paper of Whatman is used as the carrier, cut into discs with a diameter of 3.98mm, and the chromatographic paper carrier is prepared by 100U / ml lactate oxidase and pH=7.0, 100mmol / L piperazine-N, Soak in N-bis(2-ethanesulfonic acid) (PIPES) buffer for 2 hours, and dry at 20°C fo...

Embodiment 3

[0098] Urine Purification Device

[0099] The urine purification device is the same as in Example 1 in this example. The selective specific adsorbent of the present embodiment is an adsorbent composed of 0.4g neutral alumina and 0.2g purified activated carbon: wherein the purified activated carbon is treated with 10% NaOH, and the selective adsorbent is baked at 150°C for 36 hours. Activated, the activated adsorbent can completely absorb and remove interfering substances in urine such as bilirubin, vitamin C and other interfering substances.

[0100] Preparation of dry chemical reaction device

[0101] Prepare lactate oxidase pads:

[0102] The 3MM grade chromatographic paper of Whatman is used as the carrier, cut into discs with a diameter of 3.98mm, and the chromatographic paper carrier is made of 200U / ml lactate oxidase and pH=7.2, 100mmol / L piperazine-N, Soak in N-bis(2-ethanesulfonic acid) (PIPES) buffer for 2 hours, and dry at 20°C for 4 hours.

[0103] Prepare perox...

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Abstract

The invention provides a kit for detecting urinary lactic acid, creatine and beta-hydroxybutyric acid of human urine simultaneously, comprising a urine purification unit and a dry chemical reaction unit, wherein the urine purification unit is provided with a selective absorbent with combination of neutral alumina and activated carbon in weight ratio of 0.2-1:0.1-2, wherein the activated carbon is soaked by sodium hydroxide with concentration of 10-20%, and the selective absorbent is baked in a closed in a drying oven at the temperature of 70-250 DEG C for 4-72h; and the dry chemical reaction unit is a reaction box with a urinary lactic acid detection hole, a creatine detection hole and a beta-hydroxybutyric acid detection hole. The invention can detect concentration of urinary lactic acid, creatine and beta-hydroxybutyric acid of human urine simultaneously quickly and easily without help of other instruments and professional personnel so as to obtain qualitative or semiquantitative results.

Description

technical field [0001] The invention belongs to the field of kits for in vitro diagnosis, in particular to a kit capable of simultaneously detecting urinary lactic acid, creatine and β-hydroxybutyric acid in human urine. The concentration of lactic acid, creatine and β-hydroxybutyric acid in the fluid is used to evaluate the weight loss effect and exercise intensity. Background technique [0002] Obesity has attracted more and more attention from the medical community around the world. Studies have shown that obesity can induce diabetes, high cholesterol and kidney failure and many other diseases. In my country, since the reform and opening up, with the improvement of people's living standards and nutritional status, the number of obese and overweight people has increased day by day. Obesity has become a health problem that cannot be ignored in our society. [0003] Due to the increase of obese people, losing weight and losing weight have also become a fashion in our count...

Claims

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Application Information

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IPC IPC(8): G01N33/50
Inventor 许幂王加义
Owner 北京中生金域诊断技术股份有限公司
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