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Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof

A technology for detecting test paper and oxybutyric acid, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions. and other issues to achieve the effect of simple manufacturing and wide market value

Active Publication Date: 2010-10-20
苏州市玮琪生物科技有限公司
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Problems solved by technology

However, the main problems of this method are that specific equipment, venues and personnel are required, and the measurement requires a large amount of blood, which takes a long time and costs high. It is only suitable for large-scale testing places such as hospitals, and cannot Testing in small clinics is not convenient for patients to self-test at home

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  • Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof
  • Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof
  • Stabilized beta-hydroxybutyric acid detection test paper and preparation method thereof

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Embodiment Construction

[0023] Such as figure 1 A stabilized β-hydroxybutyric acid detection test paper is shown, which is composed of three layers of whole blood filter layer 1, chromogenic layer 2, and base layer 3. The whole blood filter layer 1 is composed of at least one layer of polymer Composed of film layers, the color-developing layer 2 is a water-absorbing material, and the base layer 3 is an insoluble supporting material.

[0024] The steps of the preparation method of the stabilized β-hydroxybutyric acid detection test paper are as follows: a, soak the color-developing layer 2 in the reagent solution for 2 hours, take out the cold air and dry it, b, filter the whole blood layer 1, soak and dry the color-developing layer 2 The color layer 2 and the base layer 3 are cut to the same specifications, and glued together from top to bottom;

[0025] The reagent solution includes the following components:

[0026] (1), enzyme: β-hydroxybutyrate dehydrogenase, the concentration is 10-500U / ml, di...

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Abstract

The invention relates to stabilized beta-hydroxybutyric acid detection test paper and a preparation process thereof. The stabilized beta-hydroxybutyric acid detection test paper consists of a whole blood filter layer, a developing layer and a base layer which are sequentially stacked, wherein the whole blood filter layer is formed by combining at least one polymer film layer; the developing layer is made of a hydrophilic material; and the base layer is made of an insoluble supportive material. The method for preparing the stabilized beta-hydroxybutyric acid detection test paper comprises the following steps of: a, soaking the developing layer into reagent solution for 2 hours, taking the soaked developing layer out, and drying the soaked developing layer by cold air; and b, cutting the whole blood filter layer, the soaked and dried developing layer and the base layer into the same specifications, and sequentially bonding the layers with glue from top to bottom. The test paper is not limited by conditions such as region and the like; patients can conveniently perform self-monitor at any time and at any place; the concentration of beta-hydroxybutyric acid can be detected in a short time by only using micro blood; and the test paper is simple to prepare and has wide market value.

Description

Technical field: [0001] The invention relates to a stabilized β-hydroxybutyric acid detection test paper and a manufacturing process thereof. Background technique: [0002] Ketone bodies are special intermediates produced by the normal catabolism of fatty acids in the liver, 75% of which are β-hydroxybutyric acid. The content of β-hydroxybutyric acid in normal human blood is very small. However, under certain physiological conditions (such as starvation, fasting) or pathological conditions (such as diabetes), the source of sugar or oxidative energy supply is impaired, and fat mobilization is enhanced, and fatty acids become the main energy supply material of the human body. If the amount of ketone bodies synthesized in the liver exceeds the ability of extrahepatic tissues to utilize ketone bodies, the balance between the two will be out of balance, and the blood concentration will be too high, leading to ketoneemia and ketonuria. Beta-hydroxybutyric acid is an acidic subst...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 徐华良顾瑜邹丽萍肖洁瑾
Owner 苏州市玮琪生物科技有限公司
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