Colloidal gold test paper card for detecting bladder cancer and preparation method thereof
A colloidal gold test strip, bladder cancer technology, applied in the biological field, can solve problems such as the influence of the operator's technical level on the results, the inability to monitor the patient's condition, and the difficulty in deploying the basic hospital, so as to achieve intuitive and reliable judgment, facilitate self-monitoring, and reduce invasiveness. Check the effect
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Embodiment 1
[0055] 1. Preparation of colloidal gold test paper card for detection of bladder cancer
[0056] Such as figure 1 and figure 2 As shown in 2, the test paper card includes a bottom plate 1, a sample pad 2, a colloidal gold pad 3, a chromatography pad (nitrocellulose membrane) 4, and a sample suction pad (absorbent paper or absorbent plate) 5. The base plate 1 is a base plate made of PVC material. Sample pad 2 is made of absorbent glass fibers. The colloidal gold pad 3 is made by spraying the gold-labeled antibody complex on the glass fiber membrane, with a width of 1 cm and a spraying concentration of 30 ug / ml. On the chromatography pad (nitrocellulose membrane) 4, 0.5 cm away from the colloidal gold pad, a detection zone 6 (forming a detection line) consisting of anti-APOA1 monoclonal antibody is sprayed, and the spraying concentration is 100 ug / ml. Distance detection area (detection line) 6 is close to one side 1cm of suction pad, is sprayed with the quality control area...
Embodiment 2
[0066] A method for preparing a colloidal gold test paper card for detecting bladder cancer, comprising:
[0067] 1) Preparation of colloidal gold pads
[0068] a. Pretreatment of gold pads
[0069] Cut the gold pad (made of glass fiber film or polyester film) into a size of 1cm*12cm, soak it in gold pad buffer for 10-15 minutes, dry it, and set aside;
[0070] The gold pad buffer: borate buffer + 2% BSA + 3% sucrose + 0.6M NaCl + 0.2% Tween 20;
[0071] Described borate buffer solution: the H of 0.1Mol / L 3 BO 4 +0.0025Mol / L of Na 2 B 4 o 7 10H 2 O, pH=9;
[0072] The 2% BSA is prepared with PBS, and BSA is bovine serum albumin.
[0073] Studies have found that adding sucrose can effectively slow down the retention of gold-labeled antibodies.
[0074] b. Preparation of colloidal gold solution
[0075] The container is siliconized in advance, that is, the container is soaked in 5% dichloromethylsilane in chloroform solution for 10 minutes, rinsed with triple distille...
Embodiment 3
[0088] The difference between the preparation method of the colloidal gold test paper card for detecting bladder cancer and that of Example 2 is that the detection line and the quality control line are replaced by dots, and the specific implementation method is as follows:
[0089] a. Prepare the quality control line antibody (the secondary antibody of goat anti-APOA1 polyclonal antibody) and the detection line antibody (mouse anti-APOA1 monoclonal antibody) at concentrations of 1mg / ml and 100ug / ml, respectively;
[0090] b. Take 0.3ul of the antibody solution with a pipette gun and drop it onto the NC membrane (nitrocellulose membrane)
[0091] c. Leave it to dry, and store it in a dry place at 4°C in the dark.
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