Marker group for diagnosing and distinguishing coronary arterial atherosclerosis from stable angina pectoris
A technique for coronary atherosclerosis and stable angina pectoris, which is applied in the field of biochemistry to achieve the effects of promoting standardization, high sensitivity and improving diagnostic convenience
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Embodiment 1
[0025] Example 1: Screening characterization of differential metabolites in plasma between patients with coronary atherosclerosis and patients with stable angina
[0026] 1. Objects and methods
[0027] 1. The source of the specimen
[0028] After obtaining the consent of the patients, the peripheral venous blood plasma of 480 patients with coronary atherosclerosis, 280 patients with stable angina pectoris and 350 healthy people in Jiangsu Provincial People's Hospital from September 2010 to June 2015 were collected. Confirmed by coronary angiography. The age and sex of healthy people were matched with patients with coronary atherosclerosis and stable angina pectoris. All patients with coronary atherosclerosis, patients with stable angina pectoris and healthy people have normal heart, lung, liver, kidney and hematopoietic functions.
[0029] The time of blood collection was in the morning on an empty stomach.
[0030] 2. Main reagents
[0031] Acetonitrile and formic acid ...
Embodiment 2
[0052] Example 2: Construction of ROC curves to verify the ability of six differential metabolites to diagnose coronary atherosclerosis and stable angina pectoris
[0053] Receiver operating curve (ROC) method was used for verification, and the expression levels of differential metabolites in the plasma of patients with stable angina pectoris and patients with coronary atherosclerosis were used to diagnose and distinguish patients with stable angina pectoris and patients with coronary atherosclerosis Ability. The results showed that the six differential metabolites of glutathione, β-hydroxybutyric acid, proline, fumaric acid, phenylalanine, and lactic acid alone were used to diagnose patients with stable angina pectoris and patients with coronary atherosclerosis The ability is strong, and the area under the ROC curve (AUC) is greater than 0.7, which has clinical diagnostic significance; when combined for diagnosis, with the increase of the number of combinations, the AUC furth...
Embodiment 3
[0062] Embodiment 3: the preparation of detection kit
[0063] A detection kit has been prepared based on the metabolic markers provided by the present invention, and the kit includes the following components:
[0064] Standards of metabolic markers: including glutathione, β-hydroxybutyric acid, proline, fumaric acid, phenylalanine, lactic acid, each standard is packaged separately;
[0065] Plasma metabolite extraction solvent: 100% acetonitrile and 20% acetonitrile aqueous solution (used for UPLC-Q / TOF-MS sample preparation); a mixed solution of methanol, chloroform and water in a ratio of 2.5:1:1, methoxyaminopyridine and N-Methyl-N-trimethylsilyl trifluoroacetamide (for GC-Q / MS sample preparation); 20% acetonitrile aqueous solution can be used as dissolved standard in UPLC-Q / TOF-MS screening and characterization Solvent; in GC-Q / MS screening and characterization, use the plasma metabolite extraction solvent to prepare the standard solution according to the sample preparat...
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