114 results about "Bacterial enzymes" patented technology

The invention belongs to the technical field of heavy-metal pollution restoration of soil and particularly relates to a method for restoring soil chromium-polluted soil. According to the main principle of the method, sludge, straws, the chromium-polluted soil and composite strains BYM (Bacterial Enzyme) are mixed and fermented and biological enzyme, organic sugar, and other substances which are generated in a microbial fermentation process are used for reducing hexavalent chrome contained in soil into trivalent chromium; and then chromium pollutants contained in the soil are thoroughly removed by planting a chromium hyperaccumulator. Compared with the prior art, the method disclosed by the invention belongs to an in-situ restoration technology, has the advantages of small investment, low operation cost and minimal environmental disturbance, can improve the hardening degree of the soil, enhance the loose degree of the soil and increase the content of organic matters contained in the soil and is favorable to the plant growth of the restored soil.

Detergent compositions containing an alkaline bacterial enzyme exhibiting endo-beta-1,4-glucanase activity (E.C. 3.2.1.4) and specific detergent formulations comprising less than 10 wt % zeolite and phosphate builder are described. Preferred formulations comprise surfactants selected from alkyl benzene sulphonates in combination with alkyl ethoxylated sulfates or MES or non-ionic surfactants.

The invention relates to an anti-disease greenhouse vegetable fertilizer which is characterized by comprising bacterial enzyme with effective viable count of (2.0-3.0)*10<9>CFU / 9, shale, straw powder, dry chicken manure, bone meal, fish meal, soybean meal, borax, magnesium sulfate, ferrous sulfate, potassium dihydrogen phosphate and manganese sulfate. The greenhouse vegetable fertilizer consists of bacterial enzyme, organic matters and microelements and can comprehensively meet the growth and development needs of crops and increase the output by over 15%; the fertilization cost is reduced, and the output-input ratio is increased by over 18%; and moreover, the greenhouse vegetable fertilizer enhances the ability of resisting pest and disease damage of crops, and can perfectly prevent gray mold, leaf mold, anthracnose, early blight, late blight, leaf spot disease and the like.

Bacteria-responsive core-shell nanofibers and a process for the preparation thereof are described. The nanofibers release of an antibacterial agent in response to the presence of bacteria. The core of the nanofiber comprises a biocompatible polymer together with an antibacterial agent such as a quaternary ammonium compound, for example benzyl dimethyl tetradecyl ammonium chloride (BTAC). Surrounding the core is shell comprised of a bacterially degradable polymer, which is susceptible to break-down by bacterial enzymes such as lipase, or to acidic pH conditions. The shell may comprise, for example, polycaprolactone (PCL) and poly(ethylene succinate) (PES). The nanofibers may be incorporated into wound dressings

The invention relates to a associated fermentation technique of germzymin acted as fodder additive. Its process flow is as follows: to plant multiple bacterial spawn of enzyme production and multipleready produced bacterial spawn on the slope respectively, after ripeness to be developed into spore suspension; then mixing said of each kind respectively according to proportion; hence seed culture medium to be led into enzyme production and spore suspension of ready produced and mixed bacteria spawn; finally through developing seed culture medium sets up full spore, mixing seed disinfectant of enzyme production germ and of ready produced bacterium, developing into mixture transfer agent of germ and enzyme, thereafter ferment breeding, products after ferment to be treated and become germzymin.

A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase / prephenate dehydrogenase or phosphoglucose isomerase.

The invention provides a drug for treatment of blood glucose, dyslipidemia and neurodegenerative diseases and a preparation of the drug. The drug is characterized in that the drug inhibits the activity of an intestinal bacterial BSH enzyme and increases the level of conjugated bile acid in anthe intestinal tract, thus the activity of FXR of bile acid receptors FXR is inhibited, synthesis of liverbile acid is improved, and the level of liver cholesterol and triglyceride is decreased; and the drug comprises amide derivatives form by carboxyl in ursodesoxycholic acid (UDCA) or chenodeoxycholic acid (CDCA) steroid stem nucleus and amidogen in other amino acids in addition except for glycine and taurine, and thus blood glucose, dyslipidemia, fatty liver, hepatic encephalopathy and neurodegenerative diseases such as senile dementia can be treated.

Provided herein are imaging methods for detecting, diagnosing and imaging pathogenic bacteria or a pathophysiological condition associated therewith using fluorescent, luminescent or colorimetric detection agents, e.g., fluorogenic substrates for bacterial enzymes, caged luciferins and fluorescent proteins, luciferases and enzymes expressed by recombinant bacteria. Signals emitted by the fluorescent, luminescent or colorimetric detection agents in the presence of the bacteria are compared to controls to detect and locate the pathogenic bacteria. Also provided is a method for screening therapeutic agents to treat the pathophysiological conditions by measuring fluorescence or luminescence emitted from the detection agents in the presence and absence of the potential therapeutic agent. In addition, a method for detecting a pathogenic bacteria via PET or SPECT imaging using a positron-emitting or gamma-emitting substrate for a beta-lactamase or other enzyme or protein of the pathogenic bacteria. Further provided are the fluorogenic substrates CNIR-7 or CNIR7-TAT or the radiolabeled substrates.

The invention discloses a ferment feed synergist and a preparation method thereof, relating to the technical field of feed synergists. The synergist is prepared from the following components in percentage by weight: 50 percent of water and 50 percent by weight of synergistic raw materials, wherein the synergistic raw materials comprise the following components in percentage by weight: 30 percent of rice bran, 30 percent of wheat bran, 20 percent of straw powder, 3 percent of brown sugar, 9 percent of bacterial enzymes, 7 percent of bone or shell powder and 1 percent of trace elements. The synergist can fed together with traditional feed or additive to remarkably improve the conversion rate and the utilization rate of the feed or the additive and enables the nutrient contents in the feed or additive to be truly and fully absorbed and utilized. Meanwhile, the invention can improve the balance of animal bodies, enhance the disease resistance of livestock and poultry, eliminate drug residues, ensure people's health, promote the development of the eco-organic food, eliminate bacteria breeding and create a healthy biological food chain.

Provided herein are imaging methods for detecting, diagnosing and imaging pathogenic bacteria or a pathophysiological condition associated therewith using fluorogenic substrates for bacterial enzymes. Fluorescent, luminescent or colorimetric signals emitted by substrates or enzyme products in the presence of the bacteria are compared to controls to detect and locate the pathogenic bacteria. Provided is a method for screening therapeutic agents to treat the pathophysiological conditions by measuring a signal emitted from the fluorogenic substrates or products in the presence and absence of the potential therapeutic agent. In addition, a diagnostic method for detecting a mycobacterial infection in a subject by contacting biological samples with a fluorogenic substrate and imaging for signals emitted from a mycobacterial beta-lactamase product. Provided are fluorogenic substrates CC1, CC2, CHPQ, CR2, CNIR1, CNIR2, CNIR3, CNIR4, CNIR5, CNIR5-QSY22, CNIR7, CNIR9, CNIR10, CNIR-TAT, CDC-1, CDC-2, CDC-3, CDC-4, CDC-5, XHX2-81, XHX2-91, XHX3-26, or XHX3-32 or a derivative thereof.

A functional type soilless culture substrate using mushroom dreg and biogas residue as raw materials belongs to the agricultural cultivation technical field; the mushroom dreg, the biogas residue and roseite are mixed according to a volume ratio of 1:2:1; 1% of agriculture water-retaining agent and 8% of bacterial enzyme bio-organic fertilizer are added according to weight ratio, and are fully stirred; the mushroom dreg is formed by fermenting and composting waste mushroom dregs generated by oyster mushroom, and the oyster mushroom is cultured by using the culture substrate formed by 50% crushed corncob and 50% cottonseed hulls, and the fermented and composted dregs are aired or dried to have the water content below 15%; the biogas residue is solid material obtained by a residue-liquid separation system after large scale biogas digester fermentation of cow dung generated by an intensified cattle farm, and the processed solid material is aired or dried to have the water content below 15%; the roseite is garden reseite with grain size of 2-5mm; the bacterial enzyme bio-organic fertilizer has valid viable count >= 20 million / gram, and organic matter is >=25%. The beneficial effects are that the substrate uses agriculture and animal husbandry waste like the mushroom dregs and biogas residue as main material, thus reducing environment pollution, reducing production cost, removing non-renewable resource dependency like turf, and providing water-retaining, growth assisting and anti-disease effects.

The invention discloses an LAMP kit and method for quickly detecting drug resistance gene CMY-2 of a bacterial AmpC enzyme. The kit comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. DNAs of to-be-detected bacterial genomes are extracted respectively like SEQ ID NO. 1-6, six specific primers and DNA polymerase with strand displacement activity are adopted to amplify a DNA sample at 60-65 DEG C, and a color-developing agent is added to observe color changes in a reaction tube to judge whether the amplification occurs. The LAMP kit and method for quickly detecting drug resistance gene CMY-2 of the bacterial AmpC enzyme have the advantages of quickness, efficiency, simpleness and convenience in operation, high specificity, high sensitivity, simple and convenience in detection and suitability for field testing, and is suitable for popularization and application.

The invention provides an antibacterial coating. The antibacterial coating comprises a zwitterionic polymer with a structure shown in a formula (I) or a formula (II). The zwitterionic polymer designedby the invention can conform to the development process of bacteria, a quaternary ammonium salt group with positive electricity and a phosphate radical group with negative electricity exist on the zwitterionic polymer to form a zwitterionic surface, and when the surface of a material is infected by bacteria, the surface of the material is an anti-adhesion surface initially, so that the function of bacterial adhesion resistance is achieved. Once phosphate radicals on the surface are easily released by bacterial enzymes generated by metabolism of bacteria growth and propagation, the surface isimmediately converted into a cationic sterilization surface. The construction of the surface not only effectively prevents the initial adhesion of bacteria, but also has good biocompatibility to organisms, and more importantly, when the initial defensiveness is broken through, the surface can be converted into an excellent sterilization function. The preparation process is simpler, the equipment requirement is low, the operation is easy, and the feasibility is high.

A biological organic fertilizer mainly aiming at root nematodes is prepared from an organic material, rhizoma picrorhizae, common cnidium fruit, liquorice and other raw materials. A preparation method comprises the steps: processing the raw materials; mixing the raw material medicines, and regulating the water content and the pH value; adding bacterial enzyme and mixing; stacking, sealing tightly with plastic cloth, and fermenting until a raw material color is yellowish-brown, no odor or slightly mouldy odor and fermentation odor are generated, and the surface of the stock pile has white flora; spreading out the stock pile, air-drying, crushing, bagging, and thus obtaining the biological organic fertilizer mainly aiming at the root nematodes. The biological organic fertilizer is scientific and reasonable in formula design, can make fertilizer efficiency lasting, allows microorganisms to breed fast, can relatively completely eliminate the root nematodes, cutworms or other plant diseases and insect pests, and can restore a soil structure. The biological organic fertilizer makes full use of resources, reduces environmental pollution, and can be widely applicable to all kinds of crops and commercial crops.

The invention in particular relates to the modification of a bacterial enzyme carboxypeptidease G2 (CPG2) to result in CPG2 proteins that are substantially non-immunogenic or less immunogenic than any non-modified counterpart when used in vivo. The present invention relates also to T-cell epitope peptides derived from said non-modified protein by means of which it is possible to create modified CPG2 variants with reduced immunogenicity. These polypeptides are suitable particularly for therapeutic use in humans.

The invention relates to a chromogenic medium for separating and detecting enterobacter cloacae, and in particular relates to a medium and method for specifically separating and detecting enterobacter cloacae by using beta-xylosidase and a corresponding substrate. The beta-xylosidase chromogenic substrate is decomposed in the presence of a bacterial enzyme to free chromogenic groups out, and colonies present the color of the chromogenic groups, so that the enterobacter cloacae is distinguished from other enterobacteriaceae bacteria. The medium for separating and detecting the enterobacter cloacae, disclosed by the invention, has the advantages of strong specificity, high sensitivity, easiness in operation, simplicity in result judgment and the like, and is applicable in the fields of medical clinical microbial examination, food safety microbial detection, environmental protection and the like.

Provided herein are methods for detecting, quantifying, differentiating, diagnosing and imaging pathogenic bacteria or condition associated therewith using substrates for bacterial enzymes. Fluorescent, luminescent or colorimetric signals emitted by substrates or enzyme products in the presence of the bacteria are compared to controls to detect and locate the pathogenic bacteria. Provided is a method for screening therapeutic agents to treat the pathophysiological conditions by measuring a signal emitted from the substrates or products in the presence and absence of the potential therapeutic agent and a diagnostic method for detecting a mycobacterial infection in a subject by contacting biological samples with a substrate and imaging for signals emitted from a mycobacterial beta-lactamase product. Also provided are fluorogenic substrates or substrates comprising a colored dye or a chemical reagent effective to induce a color or pH change.

The invention relates to a bacterial enzyme fermented traditional Chinese medicine for improving the growth performance of poultry and a preparation method of the bacterial enzyme fermented traditional Chinese medicine. The bacterial enzyme fermented traditional Chinese medicine mainly comprises a Chinese herbal medicine enzymolysis component and probiotics, the Chinese herbal medicine enzymolysiscomponent comprises dandelion, folium artemisiae argyi powder, angelica sinensis, pericarpium citri reticulatae, liquorice, cellulase, xylanase, acid protease, phytase and the balance of water, and the probiotics comprise bacillus subtilis, enterococcus faecalis, saccharomyces cerevisiae and lactobacillus plantarum. The preparation method of the bacterial enzyme fermented traditional Chinese medicine comprises the following steps: preparing an enzymolysis composition taking Chinese herbal medicines as main materials, preparing a probiotic seed culture solution, and mixing an enzymolysis product of the Chinese herbal medicines with the probiotic seed culture solution for fermentation to obtain the bacterial enzyme fermented traditional Chinese medicine. The bacterial enzyme fermented traditional Chinese medicine disclosed by the invention can improve the growth performance of broiler chickens, and has the effects of diminishing inflammation, resisting diseases and improving immune organ indexes of the broiler chickens.

The invention discloses fermented feed and a preparation method thereof. The fermented feed at least comprises a fermentation strain, a matrix, a nitrogen source, a carbon source and inorganic salts. A culture solution of the fermented feed is composed of, by weight, 3-7 parts of industrial milk powder, 1-2 parts of microelements, 1-5 parts of potassium humate, 1-2 parts of seaweed extract, 1-3 parts of amino acid powder, 1-3 parts of fish powder, 3-7 parts of molasses and 50-70 parts of distilled water. The fermentation strain is formed by one or more of lactic acid bacteria, photosynthetic bacteria, bacterial enzymes, bacillus subtilis, bacillus megatherium, actinomycetes, nitrifying bacteria, bacillus aceticus and the like and is applied to the matrix formed by one or more of rice husks, rice straws, wheat straws, maize straws, bagasse, wood fibers and the like to form the fermented feed by fermentation and subsequent processing, and the fermented feed can be used with traditional feed for feeding of poultries and livestock.

The invention provides a chondroitin sulfate production process. A raw material is crushed to form particles with a diameter of 3-5mm, the particles are placed into an immersion pool, water which is 7-8 times as heavy as the raw material is added into the immersion pool for immersion, the particles are sent into a pulping machine to form a raw material pulp, the raw material pulp is sent into a reaction kettle, is heated and boils, then heat preservation is carried out for 10-15 minutes, and floating oil is taken out after temperature reduction to obtain collagen thermal denaturation liquid; a PH value of thermal denaturation liquid caustic soda is adjusted, bacterial enzymes are added, heat-preservation enzymolysis is carried out for 5-6 hours, the temperature is reduced to be the same as the temperature of cold water, then the temperature is raised to 70-75 DEG C, and heat-preservation is carried out for 10-15 minutes; materials after enzymolysis are clarified and filtered, and a wet-state chondroitin course product is obtained after alcohol precipitation, dehydration and alcohol removal; decoloration, concentration, alcohol precipitation and alcohol removal are carried out on the wet-state chondroitin course product, and a chondroitin fine product is obtained after drying. Mechanical physical degradation is carried out, the chondroitin is prevented from being degraded under a high temperature, the yield rate is improved, production cost is reduced, the yield is improved, the production efficiency is improved, the production is increased and the process is scientific and environmentally friendly.

The invention discloses an active lactic acid bacteria beverage for a pet and a method for preparing the same. The lactic acid bacteria beverage comprises the following components: 1*10 to 1*10 CFU per 100 milliliters of lactic acid bacteria, 0.3 to 1 percent of emulsion stabilizer, 0.1 to 1 percent of thickening agent, 0.1 to 1 percent of sodium benzoate, 0.2 to 1 percent of acidity regulator, 8 to 20 percent of lactose, 1 to 5 percent of essence, and the balance being purified water. The invention also provides applications of the lactic acid bacteria beverage in adjusting the balance of the beneficial microflora in the intestinal canals of the pet, reducing the generation of nocuous fermentation products and harmful bacterial enzymes in the body of the pet, conditioning the digestion power of intestines and stomach of the pet, adjusting immune systems of organisms, improving the immunity of the pet, and promoting the soma of the pet.

The present invention relates to the field of poly- and oligosaccharides and their dietary effects. In particular it relates to a method of producing an alpha-glucan containing (alpha 1-> 3) linked D-glucose units. The invention also provides a branched alpha-glucan comprising alternating (alpha1-> 4) and (alpha1-> 3) glucosidic linkages and having (alpha1-> 3,4) branching points, a food composition, and the use of an alpha-glucanotransferase enzyme for reducing the digestible carbohydrates of a starch containing food material. Further aspects of the invention are a bacteria, an enzyme and anexpression vector.

The invention discloses a soil fertilizer-adding material and a preparation method thereof. The soil fertilizer-adding material is prepared from the following components in parts by weight: 20-40 parts of bovine feces, 10-30 parts of sweet potato vines, 6-12 parts of bacterial enzyme, 3-8 parts of soybean straws, 5-9 parts of leaves of camphor trees, 4-10 parts of willow bark, 5-12 parts of diammonium hydrogen phosphate, 15-35 parts of calcium superphosphate, 7-14 parts of seaweed meal and 3-9 parts of cellulose acetate. The invention further discloses a preparation method of the soil fertilizer-adding material. The method comprises the following specific steps of adding the bovine feces, the sweet potato vines, the bacterial enzyme, the soybean straws, the leaves of camphor trees and the willow bark to a fermentation tank for anaerobic fermentation for 4-8 weeks to obtain a rotten organic manure; and adding the diammonium hydrogen phosphate, the calcium superphosphate, the seaweed meal and the cellulose acetate to the rotten organic manure and stirring evenly. The soil fertilizer-adding material has the advantages that the soil fertility is effectively increased and the soil fertilizer-adding material is environmentally friendly.

The invention discloses a fertilizer synergist. The fertilizer synergist is prepared from the following raw materials in parts by weight: bensulfuron methyl, humic acid, alkanol milk powder, yellow mealworm dung, super absorbent resin, diatomite, yellow mealworm dung, sodium dodecylbenzene sulfonate, a high stake agent, brassinolide, indoleacetic acid, polyaspartic acid, hydroxylated modified lecithin, seaweed functional masterbatch, composite biomass carbon, cunninghamia lanceolata powder, ferrous sulfate, manganese sulfate, copper sulfate, azotobacter, bacterial enzyme, actinomycetes, borax,a surfactant and a penetrant. Migration and loss of nitrogen, phosphorus and potassium can be effectively reduced, the ammoniation and digestion effects of fertilizer are relieved, the release rate of nutrients is controlled, the slow-release and loss control combined effect is realized, the utilization rate of the fertilizer is increased, pollution of the fertilizer to the environment is reduced, and yield increasing of crops is promoted; and soil can be activated, hardening can be overcome, sufficient nitrogen can be provided, sufficient microbial strains can also be provided for the soil,and the crop yield can be rapidly increased.

The invention discloses a composite bacterial enzyme system with multiple effects and application thereof. The composite bacterial enzyme system provided by the invention is composed of a microbial agent and an organic matter, wherein active ingredients of the microbial agent are composed of Bacillus subtilis CGMCC No.1.1630 and Pediococcus acidilactici CGMCC No.1.2696; and the organic matter is humic acid. Experimental results show that the composite bacterial enzyme system provided by the invention can regulate plant growth, increase plant yield, prevent and control plant diseases and pests, enhance plant disease resistance, improve soil and enhance soil fertility.

The invention relates to bio-organic fertilizer and specifically relates to environment-friendly bio-organic fertilizer for roxburgh rose and a preparation method of the environment-friendly bio-organic fertilizer. The environment-friendly bio-organic fertilizer is prepared from the following components: 500-800 parts of roxburgh rose branches and leaves, 200-700 parts of mountain soil, 100-300 parts of straw, 50-150 parts of corncob, 50-150 parts of wood chips, 50-150 parts of waste materials, 15-50 parts of rice bran, 5-20 parts of bacterial enzyme, 1-5 parts of brown sugar and water accounting for 60-65% of abovementioned raw materials in total. The invention also discloses a preparation method of the environment-friendly bio-organic fertilizer for roxburgh rose. The environment-friendly bio-organic fertilizer for roxburgh rose provided by the invention has the advantages that the physical and chemical properties of soil are changed, the soil fertility is improved, and the high yield and high quality of the crops are promoted.

The invention discloses the demethylation of 5-methoxyflavonoids by bacterial enzymes, the use of these enzymes in the production of phytoestrogens in vitro, and in pharmaceutical compositions in combination with a source of methylated 5-methoxyprenylflavonoids.