80 results about "Archaeal Viruses" patented technology

The invention discloses a compound bacterium community capable of efficiently leaching a sulphide ore, and a compounding method and an application method thereof, and belongs to the technical filed of wet-process metallurgy. Aiming at a biological leaching mechanism of the sulphide ore and the physiological-biochemical characteristics of microorganisms, a community capable of efficiently leaching the sulphide ore is compounded by a plurality of mineral leaching microorganisms, wherein the mineral leaching microorganisms comprise marine bacteria which come from deep-sea hydrothermal vents and are capable of enduring high concentration sodium chloride, sulfur-oxidized bacteria, iron-oxidized bacteria and archaea which are from a freshwater environment, autotrophic bacteria and facultative heterotrophic bacteria. Not only can the difficult problem that the mineral leaching microorganisms from the freshwater environment are intolerance of sodium chloride be solved, but also microorganisms required by oxidation and dissolution of the sulphide ore and diversity of chemical reactions are guaranteed. The compound bacterium community can obviously increase leaching efficiency and leaching rate of the sulphide ore such as copper pyrites and can be applied in a leaching process and a dump leaching process of a stirring tank. A certain basis for popularization and application of biological metallurgy of the sulphide ore is provided by the invention.

A method for revealing and distinguishing paddy field formic acid utilization type methanogenic archaea in situ by adopting DNA-based stable isotope probing technology comprises the followings steps: (1), collecting a paddy soil sample; (2), performing a micro universe cultivation experiment of <13>C-formic acid; (3), performing centrifugation layering on the microbe total DNA of <13>C-formic acid cultivation soil by using an ultracentrifuge; (4), performing real-time quantitative PCR analysis and finger-print analysis on methanogenic archaea genes in layers of multiple buoyant densities after layering to judge whether the paddy soil contains the formic acid utilization type methanogenic archaea with metabolic activity. Therefore, the method can be used for keenly revealing the paddy field formic acid utilization type methanogenic archaea in situ based on the DNA-based stable isotope probing technology, and has great significance on the understanding of the paddy field nutrient cycling process driven by microbes and the cognition of the ecological functions of paddy field methanogenic archaea functional groups.

The invention discloses a compound halophilous-microorganism bacterium agent and an application thereof. Halophilous microorganisms are the microorganisms which are not inhibited or poisoned by high-concentration inorganic salts in the high-salt environment, can carry out normal growth and metabolism and comprise salt-resistant microorganisms and halophilic microorganisms. The compound halophilous-microorganism bacterium agent at least comprises two of halophilic bacteria, halophilic archaea, salt-resistant bacteria, salt-resistant archaea, halophilic fungi and salt-resistant fungi, wherein the types and the quantity of the halophilous microorganisms are at least 10, and the halophilous microorganisms at least comprise one type of uncultured microorganisms. The compound halophilous-microorganism bacterium agent disclosed by the invention has the advantages that high-efficiency and stable degradation can be carried out on organic matters under the condition of 1%-20% salt; by using the compound halophilous-microorganism bacterium agent for biochemical treatment of high-salt waste water and microorganism restoration of saline-alkaline soil, the pollution treatment cost can be reduced.

A method for quantifying ammonia oxidizing archaea in lacustrine deposit. The method comprises the following steps of (1) extracting DNA, and (2) constructing an ammonia oxidizing archaea plasmid standard substance and analyzing a sample, wherein the copy number of Ammonia oxidizing archaea in an unknown sample is obtained through processes of a detection of a Ct value of the unknown sample and a contrast of an amplification standard curve and the Ct value after the amplification standard curve is obtained. In the invention, ammonia oxidizing archaea in lacustrine deposit is detected quantificationally through a real-time fluorescent quantitative polymerase chain reaction (PCR) technology. The method is a rapid, simple and accurate method for researching a nitrogen cycle mechanism of lacustrine deposit.

The invention belongs to the field of gene engineering, and relates to a DNA polymerase gene separated from Hyperthermophilic Archaeon, a plasmid thereof, a DNA polymerase coded thereby, a preparation method of the DNA polymerase, and an application of the DNA polymerase, and concretely relates to DNA polymerase coding DNA separated from Pyrococcus yayanosii, and an application and a preparation method thereof. The sequence of the DNA polymerase coding DNA is one of a nucleotide sequence represented by SEQ ID NO.1in a sequence table, and a nucleotide sequence obtained by deleting, inserting and substituting one or more nucleotides into the sequence represented by SEQ ID NO.1. The encoded DNA polymerase has the advantages of super strong high-temperature resistance, high fidelity performance, excellent anti-inhibitor ability, and realization of a direct PCR reaction by using crude samples.

The invention belongs to the field of microbial molecular ecology detection, and particularly relates to a kit and a method for rapidly detecting archaea. According to the kit and the method for rapidly detecting archaea, the problems existing in abundance, diversity, composition and structure of archaea communities under different environments in real-time fluorescent quantitative PCR and high-throughput sequencing at present are solved, and the kit for quickly detecting archaea comprises a primer group, a DNA fragment, a PCR buffer solution and an STE buffer solution; in addition, the DGGE technology is used for rapidly detecting the archaea, the experiment cost is low, operation is easy and convenient, and meanwhile the method has the advantages of being high in specificity, easy to popularize in a laboratory, independent of large experiment equipment and the like; and the method is an efficient, rapid, economical and convenient detecting method.

The invention belongs to the field of bioengineering, and particularly relates to a preparation method of recombinant halophilic archaea protease. The method comprises the following steps: constructing a recombinant plasmid by taking a genome of Halostella sp. DL-M4 as a template and taking a pET28a plasmid as an expression vector; transforming escherichia coli cell by recombinant plasmid, inducing expression, centrifugally collecting thalli for cell resuspension and crushing, centrifuging again, taking a supernatant, adding beta-mercaptoethanol to obtain a mixed solution, enabling the mixed solution to pass through a nickel column, combining zymogen with a nickel medium for renaturation, and finally performing secondary impurity protein removal and target protein elution, performing ultrafiltration centrifugal concentration and performing gel filtration chromatography to obtain the recombinant halophilic archaea protease. The method provided by the invention has good universality, simpler purification steps and shorter production cycle, the obtained protease has high purity, and finally the halophilic archaea protease with specific enzyme activity of 1159.15 U/mg is obtained.

The invention belongs to the technical field of bioengineering, and particularly relates to halophilic archaea for degrading nitrite and an application of the halophilic archaea. According to the invention, a new species of Haloarcula is found, and the new species is named as Haloarcula sp. JB33. The strain is aerobic denitrifying bacteria, can grow within the salt concentration range of 5-25% andthe temperature range of 20-52 DEG C, and has good salt tolerance. The optimal growth temperature is 37 DEG C. When the salt concentration is 18% and the nitrite concentration is 100mg/kg-500mg/kg, the strain still normally grows and effectively degrades nitrite, and the optimal degradation rate reaches 98.59% after the strain is degraded for 96 hours. Nitrite degradation effect is obvious. The strain can be used for degrading the nitrite in foods. The halophilic archaea is particularly applied to degradation of the nitrite in high-salt pickled foods such as pickled vegetables and hams.

A synthetic charged glycolipid is described comprising a sulfated saccharide group covalently linked to the tree sn-1 hydroxyl group of the glycerol backbone of an archaeal core lipid via a beta linkage. The synthetic charged glycolipids include compounds of formula I wherein n is 0 or 1; R is hydrogen or hydroxyl; and Y is hydrogen or a sulfate group, at least one Y being a sulfate group; and including pharmaceutically acceptable salts thereof. The sulfated glycolipid produces stable archaeosomes at a mol % ratio of from 100:0 to 30:70 (sulfated glycolipid: uncharged glycolipid) and which induce a protective immune response, including CD8⁺ and CD4⁺ T cell responses. Archaeosomes comprising the sulfated glycolipids described have desirable adjuvant properties, particularly when mixed with uncharged glycolipid at a mol % ratio of about 50:50.

The invention provides microbial florawhich is composed of bacteria and archaea. The bacteria (manipulation taxon, recorded as OTUs) include Burkholderiales, Clostridiales and other strains. The archaea (OTUs) include Methanobacteriales, Methanomicrobiales, Methanosarcinales and other strains. The microbial flora can effectively reduce Cr (VI) and can completely reduce 91 microgram/mL of Cr (VI) within 49.5 h. The microbial flora can tolerate the environmental Cr(VI) concentration of 435 microgram/mL and maintain the reduction capacity. The microbial flora has the capacity of reducing Cr (VI)at 15-45 DEG C, and the efficiency of reducing Cr(VI) at 30 DEG C and 35 DEG C is the best.

The present invention provides compositions as additives for wastewater treatment. A first composition comprising archaea microorganism granules for bioaugmentation for treatment of COD in wastewater, and a second composition of activated silicate beads that allow removal of phosphate, nitrogen and suspended solids. The mixture of both compositions act synergistically by promoting organic matter degradation and allowing the removal of phosphate without consuming alkalinity of the treated solution.

The invention relates to a chitosan disaccharide deacetylase mutant, which is obtained by mutating glycine G at the 74th site of chitosan disaccharide deacetylase of extreme thermophilic archaea Pyrococcus horikoshii into alanine A. According to the invention, the enzyme activity, the expression quantity and the catalytic efficiency of the chitobiose deacetylase Dac are improved, and the problem that the efficiency of converting the chitobiose deacetylase Dac into glucosyl GlcN is not high is solved.

The invention belongs to the field of genetic engineering, and relates to a preparation method and application of novel halophilic archaea extracellular protease. The preparation method comprises thefollowing steps: firstly, obtaining a coding gene hly based on a genome of halophilic archaea DYF 46; connecting the hly to a vector by a molecular cloning technology to construct a recombinant plasmid; transforming the recombinant plasmid into a prokaryotic host, culturing the transformed recombinant host cells in an LB liquid culture medium containing kanamycin, centrifugally collecting thalli at low temperature after culture and inducible expression, resuspending in a cell lysis solution, ultrasonically crushing, centrifugally collecting supernatant for recombinant protein purification; andpurifying protein by nickel affinity chromatography, obtaining mature enzyme by a column renaturation method, and purifying by gel filtration chromatography to obtain high-purity Hly. The Hly obtained by the preparation method has excellent enzymatic characteristics, and can be applied to production and processing processes of complex and salt-containing degraded proteins such as detergents, wastewater treatment, pharmacy and environmental remediation.