63 results about "Anti proteases" patented technology

The invention discloses a hinge region modified recombinant human IgG2 antibody. Amino acids at the sites of Glu216Arg217Lys218 are deleted in a heavy chain hinge region of the antibody, and amino acid is substituted and / or deleted at the site of Cys219 and / or Cys220. The invention further provides a method for improving the antiprotease hydrolysis effect of the recombinant human IgG2 antibody. According to the IgG2 antibody provided by the invention, the heterogeneity caused by disulfide bonds in the hinge region of the antibody is eliminated, and the effect of improving the antiprotease hydrolysis effect is achieved.

Protease-like nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, protease-like fusion proteins, antigenic peptides, and anti-protease-like antibodies are encompassed. The invention also provides vectors containing a nucleic acid molecule of the invention and cells into which the vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.

The present invention relates to modified streptavidin molecules that are resistant to cleavage by Lys-C or other proteases. These modified streptavidin molecules can be produced by chemical modification of natural streptavidin, by chemical synthesis or by genetic engineering. The invention also relates to nucleic acid molecules encoding these modified streptavidin molecules, to vectors comprisingsuch nucleic acid molecules, and to cells comprising such nucleic acid molecules or vectors. The invention further relates to solid supports and kits comprising the modified streptavidin molecules. The invention also relates to the use of such modified streptavidin molecules or such solid supports for the capture / immobilization of proteins, peptides, oligonucleotides (e.g. aptamers), polynucleotides (e.g. DNA, RNA, or PNA), lipids, (poly) saccharides, carbohydrates, metabolites, drugs and small molecules, natural and synthetic molecules and to the use of these modified streptavidin moleculesor these solid supports in mass spectrometry for the identification of proteins that interact with aforementioned (bio)molecules. The invention further relates to a method for reducing background in mass spectrometry by employing the modified streptavidin molecules.

The invention relates to the field of gene engineering, in particular to beta-mannaseBA-Man5A with a wide pH range, a gene thereof and application of the gene. The invention provides the beta-mannaseBA-Man5A with the wide pH range. The beta-mannaseBA-Man5A with the wide pH range has the amino acid sequence shown as SEQ ID NO.1 or 2. The invention also provides a gene for coding the beta-mannaseBA-Man5A with the wide pH range, a recombinant vector and a recombinant strain which comprise the gene, and application of the gene. The nucleotide sequence of the gene is shown as SEQ ID NO. 3 or 4. The beta-mannaseBA-Man5A has high activity in acidic, neutral and alkaline ranges, a wide pH range, high acid and alkali resistance, high heat resistance and excellent anti-protease ability, and can be applied to industries such as animal and fish feed, food, medicines, paper making and the like. According to the technical scheme of the invention, mannase can be produced by a gene engineering method.