Antimicrobial peptide, preparation method of antimicrobial peptide, antimicrobial composition, antibacterial method and applications

Abstract

The present invention relates to an antimicrobial peptide, a preparation method of the antimicrobial peptide, a composition containing the antimicrobial peptide, an antibacterial method using the antimicrobial peptide, and applications of the antimicrobial peptide. The amino acid sequence of the antimicrobial peptide is Val Pro Tyr Ile Gln His Thr Pro Asn Leu Leu Leu Glu Gln Asn LeuLeu, wherein amino acids in the amino acid sequence are all D-type amino acids. In the case, chemical synthesis is used to efficiently obtain a large amount of high-purity total reverse D-type antibacterial peptideso as to ensure the integrity and purity of the antibacterial peptide sequence, and the total reverse D-type antibacterial peptide has high thermal stability and anti-protease degradation ability. Thebroad-spectrum antibacterial activity of the total reverse D-type antibacterial peptide in this case is greatly enhanced compared with L-type antibacterial peptides.

Description

technical field [0001] The invention belongs to the field of polypeptides, and in particular relates to an antimicrobial peptide, a preparation method of the antimicrobial peptide, an antibacterial composition, an antibacterial method and an application of the antimicrobial peptide. Background technique [0002] Antimicrobial peptides, also known as antimicrobial peptides or host defense peptides, are a class of small molecular polypeptides induced by organisms with broad-spectrum antibacterial activity and immunomodulatory activity. They are an inherent part of the body's non-specific defense system and exist in almost all In life forms (insects, amphibians, birds, fish, mammals, plants and human body), it consists of 12-60 amino acids, and the relative molecular weight is generally around 4kD. Studies have found that antimicrobial peptides have rapid bactericidal and broad-spectrum antibacterial activity, including Gram-positive bacteria, Gram-negative bacteria, multidrug-...

AI Technical Summary

Problems solved by technology

[0004] β-Casein-197 is an L-type polypeptide containing 17 amino acids isolated from human milk. It has certain antibacterial activity and is a potential therapeutic antibacterial peptide, but its antimicrobial minimum inhibitory concentration (MIC) is relatively low. High, that is, the antibacterial activity and stability are low, and it does not have the conditions to become a clinical drug

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