The present invention is directed to a
yeast strain, or strains, secreting a full suite, or any subset of that, full suite, of enzymes to hydrolyze com
starch, corn
fiber, lignocellulose, (including enzymes that hydrolyze linkages in
cellulose, hemicelhiiose, and between
lignin and carbohydrates) and to utilize
pentose sugars (
xylose and
arabinose). The invention is also directed to the set of proteins that are well expressed in
yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze
starch and
cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less
glycerol and uptake acetate. The resulting strain, or strains can also be used to produce
ethanol from
granular starch without
liquefaction.; The resulting strain, or strains, can be further used to reduce the amount of external
enzyme needed to hydrolyze a
biomass feedstock during an Simultaneous Saccharification and
Fermentation (SSF) process, or to increase the yield of
ethanol during SSF at current saccharolytic
enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on
biomass feedstock. In some aspects, host cells expressing different
heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce
ethanol from
biomass feedstock.