77results about How to "The fermentation method is simple" patented technology

The invention discloses a method for extracting roasted and fermented malt extract by ethanol and application thereof in tobacco, in particular a method for performing step-by-step enzymolysis on malt, fermenting the malt, roasting to increase aroma, and extracting the malt extract by the ethanol and application of the malt extract in tobacco aroma increase. The malt extract is prepared from the following steps of: spraying and soaking the malt; rolling and crushing the malt; performing step-by-step enzymolysis on the malt; fermenting the malt by using aroma-producing yeast; roasting to increase aroma; extracting by using the ethanol; performing solid-liquid separation; clarifying and filtering; and distilling and concentrating to obtain the malt extract. The malt extract is added into cut tobacco of cigarettes to increase the aroma, reduce the irritation and offensive taste, and improve the mouthfeel and aftertaste. The method has the advantages that: the technology of performing step-by-step enzymolysis on the malt, fermenting the malt, and extracting the malt extract by the ethanol is simple to operate, and the malt subjected to enzymolysis and fermentation is immediately roasted without enzyme inactivation treatment, which is more convenient. The extracted malt extract has the advantages of sweet roasting and brewing aroma, fine and smooth and rich aroma, and excellent aroma increasing effect in cigarettes.

The invention provides malic acid-production gene engineering bacteria Escherichiacoli BA043. The malic acid-production gene engineering bacteria is named as BA043 (Escherichiacoli BA043) and has a preservation registration number CCTCC NO: M2014034. The invention also provides a construction method of the malic acid-production gene engineering bacteria and a method for fermentation production of malic acid. Through knockout or inactivation of fumarase and fumarate reductase and through over coexpression of exogenous pyruvate carboxylase and nicotinic acid phosphoribosyl transferase, recombinant escherichia coli can reduce by-product pyruvic acid generation by glucose metabolism growth so that a malic acid yield and production intensity are greatly improved.

The present invention discloses a two-step fermentation method for efficient production of fermented soybean meal, and belongs to the technical field of solid-state microorganic fermentation. The present invention adopts soybean meal as main raw material and uses an aerobic bacteria-bacillus subtilis with high protease production capacity as a major strain of shallow dish fermentation in the first step. The fermented soybean meal with high protease activity is obtained through an aerobic fermentation for 1-2 days. The shallow dish fermented soybean meal is served as a protease inhibitor-like agent to mix with lactobacillus and other unsterilized soybean meal to conduct the second step-heap fermentation. The present invention not only can meet the requirement of mix fermentation of aerobic and anaerobic bacteria, but also solves the problem that the aerobic bacteria is difficult to apply to solid heap accumulation. The shallow dish fermented soybean meal has a protease activity of higher than 700U / g, and the soybean meal after heap fermentation has a crude protein content of higher than 50% and a small molecule peptide content higher than 10%. The mixed fermentation can improve the palatability of soybean meal for animals, promote digestion and absorption of animals, balance animal intestinal microbial environment and improve animal immunity.

The invention provides a cultivation technology method of paddy rice, which comprises the following steps: choosing variety of the paddy rice, and growing the paddy rice in an area which is suitable for growing the paddy rice; cultivating seedlings and organic fertilizer required by the seedlings, wherein the combination and the content of the organic fertilizer are as follows: 46% to 60% of excrements of livestocks, 15% to 30% of molasses powder, 5% to 15% of fermentation bovine-derived power, 3% to 10% of zeolite powder, and 0.5% to 1.6% of back spray bacterium, and the mass percent of each component equals to 100%; and management of rice seedling bed fertilizer: applying the organic fertilizer with 80-250 kg per mu for rice seedling bed base fertilizer, rational close planting should be adopted when being planted in the paddy field, and applying the organic fertilizer with 8-25 kg per mu for topdressing in the tillering stage. The method improves the unfavorable phenomena of singleness and degradation of soil nutrient, soil hardening, and significant reduction of beneficial organisms in soil caused by long-term chemical fertilizer application by applying the organic fertilizer, can improve the crop quality, increases the calcium content of crop (rice), and increases the agricultural product nutrition, and improves the ecological environment. Raw materials of the method can be obtained easily; the fermentation method is simple and easy to operate; the cost of fertilization is lowered.

The invention discloses a genetic modification method for a high-yield glutathione strain, which adopts Pichiapastoris as an improved object. The genetic modification method for the high-yield glutathione strain comprises four steps. The Pichiapastoris per se has good safety. A yeast culture medium does not contain toxic substances and pyrogens. The genetic modification method for the high-yield glutathione strain has the advantages of simpleness and convenience, readily available fermentation raw materials, energy saving, high yield and low cost. A promoter selected by the method does not need to be induced by methanol. The fermentation process is simple and the yield is high. The method reduces the use of the precursor in the strain genetic modification process so as to reduce the production cost.

The invention relates to the technical field of preparation of microbes and specifically relates to a method for efficiently improving the germination rate of Bacillus subtilis. According to the method provided by the invention, glycerine is added in a Bacillus subtilis fermentation broth as a fermentation strain, an inorganic salt solution capable of improving the spore germination rate is added when the fermentation strain is cultured for 15-18 hours, and the inorganic salt solution contains the following components in percentage by weight: 0.12-0.125% of MgSO4.7H2O, 0.01-0.015% of MnSO4.H2O, 0.05-0.1% of CaCl2, 0.005-0.008% of FeSO4.7H2O, 0.015-0.02% of KI and 0.035-0.04% of KH2PO4. The fermentation of the strain obtained by the method can achieve 5.8*10<11> CFU / ml, the number of spores is 5.49*10<11> CUF / ml, and the spore germination rate is 94.7%.

The invention discloses a profitable soil microorganism mixed fermentation method. The method includes the steps of 1) activating paecilomyces lilacinus, issatchenkia orientalis and bacillus licheniformis on a PDA (potato dextrose agar) medium, a YPD (yeast extract peptone dextrose) medium and a nutrient agar medium respectively, and transferring the three activated strains to corresponding liquid media so as to prepare seed fermentation broth; 2) inoculating 10% of each of the three strains into a mixed fermentation medium, and controlling the living bacteria count of the bacillus licheniformis to be one order of magnitude higher than those of the paecilomyces lilacinus and the issatchenkia orientalis according to the relation between the living bacteria count of the seed fermentation broth and an OD600 (optical density 600) value. The profitable soil microorganism mixed fermentation method has the advantages that a mixed fermentation medium formula is simple, production cost is reduced, equipment utilization rate is increased, and accordingly the profitable soil microorganism mixed fermentation method is suitable for large-scale production of fermented products; the fermented products are capable of improving soil flora balance, promoting growth and increasing production and can be used for producing biofertilizer and biopesticide by being compounded with organic materials, soil improvement agents and the like.

The invention provides a genetic engineering bacterium strain for producing succinic acid. The genetic engineering bacterium strain is named as Escherichia coli BA016, the preserving number registration number CCTCC NO is M 2012350. The invention further provides a construction method of the strain and a method for producing succinic acid by fermentation, recombinant escherichia coli can grow by glucose metabolism through joint excessive expression of exogenous pyruvic carboxylase and nicotinic acid ribose phosphate transferase, generation of by-product pyruvic acid is reduced, and accordingly the yield and production intensity of succinic acid are greatly improved.

The invention discloses a gamma-polyglutamic acid synthetic bacteria Bacillus lincheniformis CDL-1 and a fermentation method thereof. The gamma-polyglutamic acid synthetic bacteria can synthesize gamma-polyglutamic acid with the molecular weight of 300-350 KD, has narrow molecular weight distribution and easily-controlled rheology, is easy to process and modify by a chemical reagent and can be better applied to different fields; and in addition, the gamma-polyglutamic acid synthetic bacteria Bacillus lincheniformis CDL-1 has the advantages of stable passage, simple and easy culture method andgood application prospect.

The invention discloses bacillus subtilis for high-yield gamma-polyglutamic acid and a fermentation method of the bacillus subtilis. The preservation number of the bacillus subtilis is CGMCC NO: 9383,and the bacillus subtilis is preserved in China General Microbiological Culture Collection Center on June 25th, 2014. The gamma-polyglutamic acid is fermented and extracted through the four steps ofstrain activation, seed culture, tank loading fermentation and extraction of the gamma-polyglutamic acid, the fermentation method of the bnacillus subtili is simple, the production cost is low, the fermentation condition is easy to control, the purity of the fermentation product is high, the yield of the gamma-polyglutamic acid is as high as 56.5 g / L, and the bacillus subtilis can be convenientlyapplied to the production of the gamma-polyglutamic acid.

The invention belongs to the technical field of fermentation engineering, and especially relates to a fermentation medium and a fermentation method for producing aflatoxin B1. The fermentation medium of aflatoxin B1 contains a yeast extract which accounts for 1-5% of the total weight of the medium; the fermentation medium comprises a recovery medium, a solid fermentation medium or a growth promotion nutrient solution. The fermentation method of aflatoxin B1 comprises two steps of strain recovery and solid fermentation culture; aspergillus flavus is employed for fermentation; the fermentation medium provided by the invention is employed for strain recovery and solid fermentation culture. According to the medium and culture procedure provided by the invention, the concentration in a solid air-dried substance of the fermentation medium of aflatoxin B1 is up to 7349 ppb; when the fermentation medium and the fermentation method of the invention are compared with the prior art with respect to fermentation titer, significant advancement is provided.

The invention discloses a strain of lactobacillus plantarum Kmust-Li01. A preservation number of the lactobacillus plantarum Kmust-Li01 in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms (CGMCC) is CGMCC No.21266. The strain disclosed by the invention is applied to inhibition of pathogenic microorganisms and has an inhibiting action on Escherichia coli (Escherichia coli), Aeromonas hydrophila (Aeromonas hydrophila), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Streptococcus agalactiae (Streptococcus agalactiae), Staphylococcus aureus (Staphylococcus aureus) and Shigella Castellai (Shigella Castellai). The invention also provides a high-density fermentation method of the strain. The viable count in fermentation liquor prepared by the method can reach more than 10<11> magnitude; and the method provided by the invention is simple and is easy to operate and suitable for industrial production and market popularization and application.

The invention discloses a transglutaminase mutant expressed in an active form, belonging to the fields of enzyme engineering and fermentation engineering. According to the technical scheme disclosed by the invention, site-directed mutation is carried out on a mature region of transglutaminase, so that amino acid residues near the active site of the transglutaminase are changed, the catalytic efficiency of transglutaminase is improved, and recombinant bacterium hpro-E300W with the enzyme activity of the transglutaminase further improved is obtained. The fermentation enzyme activity of a recombinant bacterium hpro-E300W fermentation tank can reach 59.85U / mL, which is the maximal value of the currently reported fermentation level. In addition, by inserting yarrowia lipolytica endogenous proteinase Kex2 recognition sites in transglutaminase, the active expression of the transglutaminase in the yarrowia lipolytica is embodied. During the fermentation production, little impure protein is generated, the purification is simple, and the cost of industrial production of transglutaminase can be reduced.

The invention discloses a method for improving the content of ergosterol in liquid fermentation products of phellinus igniarius. The method comprises the following steps: (1) preparing a seed solution, namely inoculating activated phellinus igniarius strains onto a liquid seed culture medium added with cyclocarya paliurus extracts and culturing for 3 days-7 days to obtain the seed solution; and (2) carrying out liquid fermentation culture on phellinus igniarius, namely inoculating the seed solution obtained in the step (1) which accounts for 5%-20% of the volume of a liquid fermentation culture medium onto the liquid fermentation culture medium, culturing for 1 days-4 days, adding ubiquitin protein and then continuously culturing for 2 days-4 days, and obtaining liquid fermentation products of phellinus igniarius after the fermentation is completed. According to the characteristic of the synthetic metabolic pathway of ergosterol in liquid fermentation products of medicinal fungi phellinus igniarius, by adding the cyclocarya paliurus extracts and ubiquitin protein at a specific fermentation stage, the content of phellinus igniarius ergosterol is substantially improved.

The invention discloses a preparation method and application of fermentation products of an edible and medical fungus. On the basis of the characteristics of anabolism route of fermentation products (kaempferol and sinapic acid) of a medical fungus (pycnoporus cinnabarius), seeds of staphylea bumalda are added in the solid fermentation phase, annexin is added in a special fermentation phase, the content of kaempferol and sinapic acid in the liquid fermentation product of pycnoporus cinnabarius is effectively increased; and a novel method is provided for obtaining a product rich in kaempferol and sinapic acid. The edible and medical fungus fermentation product is rich in kaempferol and sinapic acid, can be used to produce health care products and animal feeds, and can also be applied to health care products and animal feeds.

The invention discloses a process which uses pGAP as a promoter and Pichia Yeast GS115 as an engineering bacterium to perform fermentation expression on an intestinal trefoil factor (ITF) in a fermentation tank. The method comprises the following steps: the recombinant Pichia Yeast expression vector pGAPZ alpha A-ITF is firstly constructed and then recombined in Escherichia coli Top10 to amplify, Escherichia coli plasmid is extracted and linearized to be transformed in Saccharomyce GS115, a high copy transformant is screened according to the zeocin resistance gene carried by the vector to perform constitutive expression, a high expression pilot test engineering bacterium is screened and fermented in a culture medium for 2 days to perform secretory expression on the ITF; and the target protein ITF of which the purity is more than 95% is obtained through purification treatment, wherein the output is about 200mg / L and the yield is about 50%. The intestinal trefoil factor recombinant expression vector has low production cost, short fermentation period and simple purification method; methanol induction is not adopted, the protein expression level is high; and a good foundation is laid for the large-scale industrial production of ITF.

The invention mainly relates to the field of food processing technology and discloses a fermentation method of a konjak beverage. The fermentation method comprises the following steps: raw material crushing, raw material enzymolysis, primary fermentation, secondary fermentation, third fermentation, and nutrition strengthening and packaging. The method is simple and is suitable for batch fermentation production. The konjak beverage is sour and sweet and proper, has aromatic flavor, has moderate viscosity, has stable state, and is fine and smooth. Deep processing ways of konjak are increased. Economic income of farmer households is raised by 13.5%. Multiple fruits and vegetables enrich the color of the beverage and increase fragrance and nutrients. Absorption is promoted, body is strengthened, and diseases are minimized. After enzymolysis of fruits and vegetables, microstructure can be decomposed. Thus, extraction rate is raised, fragrance and nutrition of the beverage are increased, health-care function is enhanced, the gastrointestinal function is increased, toxin is expelled and skin is beautified, and resistance is enhanced. After addition of konjak, natto fermentation is carried out. Therefore, the tradition that natto is only soybean is changed, content of nattokinase in the beverage is increased, toxicity of konjak is reduced, and the beverage's function of protecting heart and cerebral vessels is increased.

A fermented chaga wine and a preparation method thereof relate to the beverage field, in particular to a fermented chaga wine and a preparation method thereof. Aiming at the shortcoming of low utilization rate of Chaga, a fermented Chaga wine with high utilization rate of Chaga, simple formula, easy absorption by human body, improvement of human immunity and intestinal function and its preparation method are provided. The fermented chaga wine is composed of glutinous rice, chaga, licorice and pine buds, and its mass ratio is glutinous rice: chaga: licorice: pine buds=220‑270:80‑110:0.5‑2:28‑33. The present invention adopts Chaga antler and glutinous rice, licorice, and pine buds to be fermented in a certain proportion, which is easy for human body to absorb, can improve human body immunity, improves intestinal function at the same time, and has a strong synergistic effect; adopts the fermentation method, so that the nutritional components in the formula can be completely retained , fully utilized; the fermentation process is simple, and the preparation process and products do not have any additions.

The invention discloses a solid fermentation method of a straw fermentation bio-additive. The method is characterized by comprising the following steps of: mixing solid media according to the ratio of water to bean pulp powder to wheat bran powder to cane sugar to urea being 400:200:200:10:5; split charging into wild-mouth bottles after uniformly mixing; sterilizing for 20 minutes at the temperature of 121 DEG C; inoculating a straw fermentation bio-additive liquid strain into each bottle according to the proportion of 10-20 percent; and carrying out static cultivation for 48-72h at the temperature of 35 DEG C, wherein after natural airdrying, the microbial content in fungicide reaches 700*108 / g. According to the method, the production cost can be reduced, the product quality and the effectiveness are improved; and when the method is applied to water body purification, the nitrite in a water body can be degraded, a pH value of the water body is reduced, the water body is purified, and wide application prospect is realized.

The invention discloses a bio-organic compound fertilizer raw material composition, a bio-organic compound fertilizer and a preparation method of the bio-organic compound fertilizer. The bio-organic compound fertilizer raw material composition comprises the following ingredients: 100 weight parts of coal slime, 38 to 175 weight parts of straw smashed materials, 63 to 275 weight parts of feces of livestock and poultry and 0.005 to 0.025 weight parts of organic material decomposition agents. The preparation method of the bio-organic compound fertilizer comprises the steps that firstly, straws and the feces of livestock and poultry are smashed and are then uniformly mixed with the coal slime and the organic material decomposition agents, and the bio-organic compound fertilizer can be prepared through accumulation and fermentation. The coal slime generated in a coal production process can be fully utilized; the environment pollution is reduced; in addition, a fermentation method is simple and convenient; the equipment investment is low; the popularization and the scale production are easy; the physicochemical property of soil can also be improved; the organic matters of the soil are increased.

The invention relates to a strain capable of producing carboxyethylhydantoinase. The strain capable of producing carboxyethylhydantoinase is characterized in that classification name of the strain is alcaligenes faecalis subsp.faecalis, and the strain is preserved in China General Microbiological Culture Center on May 13th, 2013 with the preservation number of CGMCC No.7585. The invention also discloses an application of the strain capable of producing carboxyethylhydantoinase. Activity of carboxyethylhydantoinase produced by the strain alcaligenes faecalis subsp.faecalis is 0.064U / ml, the activity of purified carboxyethylhydantoinase can be 0.61U / ml, and enzyme activity of a constructed genetically engineered bacterium is 3.06U / ml, and a fermentation method of recombinant bacteria is simple. Conversion rate of substrate is high, the conversion rate of the substrate after reaction is carried out for 2 hours is 95%, the conversion rate of the substrate after reaction is carried out for 4 hours is 99.98%, and the strain has a better application prospect in catalyzing synthesis of carboxyethylhydantoinase.

The invention discloses a method for producing succinic acid by means of fermentation. The method includes steps of constructing acid-producing bacterial strains; producing the succinic acid from the recombinant bacterial strains by means of fermentation. Original bacterial strains for constructing the recombinant bacterial strains are actinobacillus succinogenes NJ113 (with a preservation number of CGMCC No.1716). The method for producing the succinic acid has the advantages that processes for acquiring the acid-producing bacterial strains are simple and convenient, fermentation processes are simple and feasible, the method is high in acid-producing capacity, the production cost can be greatly reduced, and economic benefits can be increased; the recombinant bacterial strains can grow in synthetic culture media without culture media with added exogenous glutamic acid and are high in succinic acid yield; the 19.53 g / L succinic acid can be produced after 27 g / L glucose which is used as a carbon source is fermented in an anaerobic serum bottle for 72 h, and the succinic acid yield can reach 72.33%.

The invention relates to a method for producing a biosurfactant through strain fermentation. A gas medium with high oxygen content supplies oxygen to a fermentation system, so that the ventilation volume and a stirring speed of a fermentation tank are substantially reduced, and an overflow-free fermentation process of the biosurfactant is realized in a conventional stirring reactor; the method hasthe advantages of high production rate, high substrate conversion rate and the like; in the last phase of fermentation, general air is used as a medium; a product in fermented liquid is concentratedby adopting a foam separation technology; a new process beneficial to industrial production of the biosurfactant is provided.

The invention discloses a method for increasing the content of hyperoside in a phellinus igniarius liquid fermentation product. The method comprises the following steps: (1) preparing a phellinus igniarius seed liquid; (2) preparing a polyporus picipes karst seed liquid; (3) firstly inoculating the polyporus picipes karst seed liquid accounting for 1%-10% of the volume of the fermentation culture medium to a fermentation culture medium, culturing for 1-4 days at a temperature of 22 to 30 DEG C, next, regulating the temperature to 25 to 29 DEG C, inoculating the phellinus igniarius seed liquid accounting for 5%-20% of the volume of the fermentation culture medium to the fermentation culture medium and then further culturing for 2-5 days, thereby finally obtaining the phellinus igniarius liquid fermentation product after fermentation is terminated. According to the characteristics of the anabolic pathway of the liquid fermentation product hyperoside of the medical fungus phellinus igniarius, the method for increasing the content of the hyperoside in the phellinus igniarius liquid fermentation product is capable of greatly improving the content of the hyperoside in the phellinus igniarius mycelium by firstly generating an intermediate product by growing the polyporus picipes karst by use of a carbon source, and then adding the phellinus igniarius liquid strain and converting the intermediate product generated by the polyporus picipes karst into the desired metabolic end product by virtue of the liquid fermentation of the phellinus igniarius.

The invention belongs to the technical field of fermentation engineering and in particular relates to an AFG1 (Aflatoxin G1) toxin-producing fermentation method of aspergillus parasiticus. The AFG1 toxin-producing fermentation method of the aspergillus parasiticus comprises the following steps: a, carrying out reviving proliferation on aspergillus parasiticus spores by adopting an enrichment culture medium; b, carrying out solid fermentation culture by adopting a solid fermentation culture medium, wherein components of each of the enrichment culture medium and the solid fermentation culture medium contain yeast extract which accounts for 1 percent to 3 percent of the total amount of the culture medium. According to the AFG1 toxin-producing fermentation method provided by the invention, theproblems that AFG1 in grains which are naturally infected with mildew at present is low and not uniform and long time is consumed and the like are solved.

The invention provides a microbial agent for preventing and treating southern blight of crops and a preparation method of the microbial agent, and belongs to the technical field of biological products. The microbial agent comprises the following components in parts by weight: 15-25 parts of trichoderma, 15-25 parts of bacillus subtilis, 25-35 parts of saccharomycetes and 1500-2500 parts of an amino acid; the preparation method of the microbial agent comprises the following steps: adding trichoderma, bacillus subtilis, saccharomycetes and amino acid into a fermentation tank, adding 7000-8000 parts of water, uniformly stirring, and carrying out anaerobic fermentation at 22-27 DEG C for 40-60 hours, thereby obtaining the microbial agent. The microbial agent disclosed by the invention containsmetabolic products of three kinds of microorganisms and three active microorganisms, and ketone substances, such as eurycomanone, are generated in fermentation metabolites; because of cooperation ofthe various fermentation products and the three microorganisms, crop southern blight can be prevented and treated effectively.

The invention belongs to the technical field of microorganisms and fermented fertilizers, and particularly relates to a preparation method and application of an environment-friendly probiotic compoundbacteria solution. According to the invention, by 1kg of raw materials, 10g of Bacillus mucilaginosus solution, 10g of Thermoanaerobacterium sp.L-D solution, 50g of rice chaff, 100g of vinasse, 100gof sauce residues, 100g of ammonium phosphate, 100g of monopotassium phosphate, 100g of corn flour, 250g of plant ash, 100g of pickled Chinese cabbage juice and 80g of brown sugar are weighed to prepare brown sugar are weighed to prepare the bacteria solution. Probiotics are used as a carrier, and proper substances are added, so that a large number of enzymes in the bacteria solution are promotedto be generated to decompose the odor, and thus, the problems of air pollution and harmless treatment of straw are effectively solved. Excrement treated by the product can also be mixed with the strawfor fermentation to produce biological fertilizer. The product provided by the invention can be used for efficiently treating atmospheric pollution generated in the breeding industry to reduce the prevalence of viruses, and has very important significance for application of the probiotics in the field of organic fertilizer.

The invention belongs to the technical field of fermentation engineering, and discloses paenibacillus ehimensis HD for producing antibacterial peptide and application of the paenibacillus ehimensis HD. The strain is preserved in China General Microbiological Culture Collection Center on August 7, 2020, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.20505. The paenibacillus ehimensis HD for producing the antibacterial peptide is high in growth speed, good in adaptability and low in fermentation cost, and the antibacterial peptide produced with the fermentation method is good in stability and wide in antibacterial spectrum, can inhibit various bacteria and fungi, particularly shows excellent antifungal activity, and can effectively inhibit the activity of saccharomycetes and filamentous fungi; and the strain can also be used for producing biological preservatives and antibacterial agents, and the obtained product is used in the biological control fields of food preservation, antibiotic-free breeding of livestock and poultry and the like.