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Lactobacillus plantarum, preparation method therefor and application of lactobacillus plantarum

A technology of Lactobacillus plantarum and microbial strains is applied in the directions of biochemical equipment and methods, microorganism-based methods, applications, etc., and can solve the problems of low production efficiency, inconvenient filling materials, difficult fermentation methods, etc. The effect of inhibiting pathogenic microorganisms and easy operation

Pending Publication Date: 2021-09-17
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the production of domestic probiotics mainly adopts ordinary fermentation technology, and its production efficiency is relatively low, and the nutrients in the medium cannot be fully utilized. It is still difficult for Lactobacillus plantarum to achieve high-density fermentation. Medium and fermentation method, lack of nutrition and accumulation of metabolites in the late stage of fermentation inhibit the growth of bacteria, and Lactobacillus plantarum is usually anaerobic fermentation, the inconvenient filling material makes it difficult to achieve high-density fermentation

Method used

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  • Lactobacillus plantarum, preparation method therefor and application of lactobacillus plantarum
  • Lactobacillus plantarum, preparation method therefor and application of lactobacillus plantarum
  • Lactobacillus plantarum, preparation method therefor and application of lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Isolation and identification of Lactobacillus plantarum

[0020] 1. Take the intestinal mucosa homogenate of healthy pigs, and then dilute it with sterile normal saline by 10-fold dilution method, take 10 -6 ~10 -8 Spread the dilution in the dilution range on the MRS solid plate medium, and incubate at a constant temperature of 37°C for 24 hours; pick a typical single colony, and repeatedly streak and separate until a pure strain is obtained; the MRS liquid medium consists of peptone 10g / L, beef extract 10g / L, yeast extract powder 5g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, Tween 80 1mL / L, 1000mL distilled water, adjust the pH to 6.2~6.4, and sterilize at 115°C for 20min.

[0021] The strain is cultured in MRS liquid medium, the cells are short rod-shaped, see figure 2 , cultured on MRS agar medium plate, the colony is milky white...

Embodiment 2

[0036] Example 2: Three-stage differential feeding high-density fermentation culture of Lactobacillus plantarum Kmust-Li01

[0037] (1) Components and content of MRS liquid medium for high-density fermentation of Lactobacillus plantarum

[0038] Peptone 10g / L, beef extract 10g / L, yeast extract powder 5g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, Tween 80 1mL / L; prepare the fermentation medium and lower the medium to the temperature required for cultivation;

[0039] (2) Inoculate Lactobacillus plantarum Kmust-Li01 into MRS liquid medium at 37°C for 15 hours with an inoculation amount of 5.0% to obtain a seed solution;

[0040] (3) Inoculate the seed solution of step (2) into the fermentation medium of step (1) with an inoculation amount of 5.0%, and ferment under the conditions of 37°C, pH 6.2-6.4, stirring at 150 rpm, and dissolved oxygen less than 1% , ...

Embodiment 3

[0042] Example 3: Three-stage differential feeding high-density fermentation culture of Lactobacillus plantarum Kmust-Li01

[0043] (1) Components and content of MRS liquid medium for high-density fermentation of Lactobacillus plantarum

[0044] Peptone 10g / L, beef extract 10g / L, yeast extract powder 5g / L, glucose 20g / L, dipotassium hydrogen phosphate 2g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, Tween 80 1mL / L; prepare the fermentation medium and lower the medium to the temperature required for cultivation;

[0045] (2) Activate the cryopreserved Lactobacillus plantarum Kmust-Li01 on the MRS agar plate medium for three times, pick a single colony and inoculate it in the deep MRS liquid test tube, cultivate it at 37°C for 18 hours, and transfer it to the MRS liquid medium, cultured at 37°C for 12 hours as seed solution;

[0046] (3) Inoculate the seed solution of step (2) into the fermentation medium of st...

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Abstract

The invention discloses a strain of lactobacillus plantarum Kmust-Li01. A preservation number of the lactobacillus plantarum Kmust-Li01 in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms (CGMCC) is CGMCC No.21266. The strain disclosed by the invention is applied to inhibition of pathogenic microorganisms and has an inhibiting action on Escherichia coli (Escherichia coli), Aeromonas hydrophila (Aeromonas hydrophila), Pseudomonas aeruginosa (Pseudomonas aeruginosa), Streptococcus agalactiae (Streptococcus agalactiae), Staphylococcus aureus (Staphylococcus aureus) and Shigella Castellai (Shigella Castellai). The invention also provides a high-density fermentation method of the strain. The viable count in fermentation liquor prepared by the method can reach more than 10<11> magnitude; and the method provided by the invention is simple and is easy to operate and suitable for industrial production and market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of plant lactobacillus, and in particular relates to a strain of plant lactobacillus and a preparation method and application thereof. Background technique [0002] Since the 20th century, with the discovery of antibiotics, it has promoted the great progress of human medicine and livestock and poultry breeding industry, thus antibiotics have become the first choice for antibacterial treatment of diseases. Although the use of a large number of antibiotics has continuously benefited the aquaculture industry, the irregular use of antibiotics has led to the emergence of a large number of drug-resistant strains. Therefore, seeking alternatives to antibiotics is an urgent problem that we need to solve. [0003] Probiotics can be directly fed to animals, and are characterized by safety, reliability and excellent performance. Probiotics play an important role in disease prevention and treatment, and are mainly loca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/25
CPCC12N1/20A23K10/18
Inventor 邓先余周晨烨李秋月谭春艳林连兵张棋麟
Owner KUNMING UNIV OF SCI & TECH
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