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Method for improving content of ergosterol in liquid fermentation products of phellinus igniarius

A technology for liquid fermentation and ergosterol, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve high efficiency, good industrial application prospects, and good repeatability.

Active Publication Date: 2015-01-14
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Ubiquitin protein is used in the liquid fermentation production of Phellinus Phellinus to increase its ergosterol content, which has not been reported at home and abroad

Method used

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  • Method for improving content of ergosterol in liquid fermentation products of phellinus igniarius

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Material preparation

[0039] Pectinase (with an enzyme activity of 50,000 U / g) and cellulase (with an enzyme activity of 30,000 U / g) were provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0040] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0041] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium, and activated culture was carried out at a culture temperature of 25° C. for 7 days to obtain activated Phellinus strains bacterial blocks.

[0042] The preparation method of Cyclocarya paliurus extract comprises: weighing a certain amount of Cyclocarya paliurus leaves, pulverizing through 100 meshes, first adding 70% ethanol aqueous solution at 60 Extract at ℃ for 1.0 h, filter to obtain the supernatant, concentrate the supernatant and vacuum freeze-dry to obtain the extract Ⅰ; add distilled water accounting for 10 times the weight of the leaves of C...

Embodiment 2

[0054] 1. Material preparation

[0055] Pectinase (enzyme activity: 30,000 U / g) and cellulase (enzyme activity: 20,000 U / g) were provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0056] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0057] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium, and activated culture was carried out at a culture temperature of 28° C. for 9 days to obtain activated Phellinus strains bacterial blocks.

[0058] The preparation method of Cyclocarya paliurus extract comprises: weighing a certain amount of Cyclocarya paliurus leaves, pulverizing through 60 meshes, first adding 80% ethanol aqueous solution at 80 Extract at ℃ for 2 hours, filter to obtain the supernatant, concentrate the supernatant and vacuum freeze-dry to obtain the extract Ⅰ; add distilled water accounting for 20 times the weight of the Cyclocarya paliurus leaves to ...

Embodiment 3

[0070]1. Material preparation

[0071] Pectinase (enzyme activity: 40,000 U / g) and cellulase (enzyme activity: 20,000 U / g) were provided by Guangxi Pangbo Bioengineering Co., Ltd.

[0072] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0073] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium, and activated culture was carried out at a culture temperature of 30° C. for 4 days to obtain activated Phellinus strains bacterial blocks.

[0074] The preparation method of Cyclocarya paliurus extract comprises: weighing a certain amount of Cyclocarya paliurus leaves, pulverizing through 40 meshes, first adding 60% ethanol aqueous solution at 70 Extract at ℃ for 1.5h, filter to obtain the supernatant, concentrate the supernatant and vacuum freeze-dry to obtain the extract I; add distilled water accounting for 15 times the weight of the leaves of Cyclocarya paliurus to t...

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Abstract

The invention discloses a method for improving the content of ergosterol in liquid fermentation products of phellinus igniarius. The method comprises the following steps: (1) preparing a seed solution, namely inoculating activated phellinus igniarius strains onto a liquid seed culture medium added with cyclocarya paliurus extracts and culturing for 3 days-7 days to obtain the seed solution; and (2) carrying out liquid fermentation culture on phellinus igniarius, namely inoculating the seed solution obtained in the step (1) which accounts for 5%-20% of the volume of a liquid fermentation culture medium onto the liquid fermentation culture medium, culturing for 1 days-4 days, adding ubiquitin protein and then continuously culturing for 2 days-4 days, and obtaining liquid fermentation products of phellinus igniarius after the fermentation is completed. According to the characteristic of the synthetic metabolic pathway of ergosterol in liquid fermentation products of medicinal fungi phellinus igniarius, by adding the cyclocarya paliurus extracts and ubiquitin protein at a specific fermentation stage, the content of phellinus igniarius ergosterol is substantially improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a method for increasing the content of ergosterol in a liquid fermented product of Phellinus Phellinus. Background technique [0002] Phellinus igniarius belongs to Basidiomycotina, Phellinus class, Rustiaceae, Pinifera. Phellinus extract has significant effects in the clinical application of inhibiting cancer cell metastasis and preventing cancer recurrence after surgery. It is currently a medicinal fungus with the highest efficiency among internationally recognized biological cancer treatment agents. [0003] Due to the particularity and complexity of the physiological state and the constraints of the external environment, Phellinus seldom forms fruiting bodies in nature, especially it takes many years to form usable fruiting bodies. Moreover, artificial cultivation is extremely difficult, the cultivation conditions are harsh, and the gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00C12R1/645
Inventor 程俊文贺亮胡传久魏海龙付立忠李海波
Owner ZHEJIANG FORESTRY ACAD
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