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Method for increasing content of hyperoside in phellinus igniarius liquid fermentation product

A technology of liquid fermentation and hyperoside, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems that hyperoside has not been reported, and achieve good industrial application prospects and good repeatability , The effect of controllable fermentation process

Active Publication Date: 2015-01-14
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no reports at home and abroad on the method of improving the content of hyperin in the liquid fermented product of Phellinus Phellinus

Method used

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  • Method for increasing content of hyperoside in phellinus igniarius liquid fermentation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Material preparation

[0035] PDA plate medium: potato 200g, glucose 20g and agar 15g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0036] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium, and activated culture was carried out at a culture temperature of 25° C. for 7 days to obtain activated Phellinus strains bacterial blocks.

[0037] Inoculate the Pseudopolypore strains preserved on the slant on the PDA plate medium for activation culture at a culture temperature of 25° C. for 7 days to obtain the activated Pseudopolypore strains bacterial blocks.

[0038]The preparation method of the Chinese mandarin tree extract comprises: weighing a certain amount of Chinese mandarin plant leaves, pulverizing through 100 meshes, first adding an ethanol aqueous solution with a mass percentage concentration of 70% that accounts for 13 times the weight of the mandarin plant leaves, and extracting at 60°C After 1.0h, f...

Embodiment 2

[0052] 1. Material preparation

[0053] PDA plate medium: potato 200g, glucose 20g and agar 20g, dilute to 1000ml with water, natural pH, sterilize at 121°C for 20min.

[0054] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium, and activated culture was carried out at a culture temperature of 28° C. for 9 days to obtain activated Phellinus strains bacterial blocks.

[0055] Inoculate the Pseudopolypore strains preserved on the slant on the PDA plate medium for activation culture at a culture temperature of 27° C. for 8 days to obtain activated Pseudopolypore strains bacterial blocks.

[0056] The preparation method of the Chinese mandarin tree extract comprises: weighing a certain amount of Chinese mandarin plant leaves, pulverizing them into 60 meshes, first adding 80% ethanol aqueous solution which accounts for 16 times the weight of the Chinese mandarin plant leaves, and extracting at 80°C After 2 hours, filter to obtain the supernatant, ...

Embodiment 3

[0070] 1. Material preparation

[0071] The PDA plate medium is the same as in Example 1.

[0072] The Phellinus strains preserved on the slant were inoculated on the PDA plate medium, and activated culture was carried out at a culture temperature of 30° C. for 4 days to obtain activated Phellinus strains bacterial blocks.

[0073] Inoculate the Pseudopolypore strains preserved on the slant on the PDA plate medium for activation culture at a culture temperature of 30° C. for 4 days to obtain activated Pseudopolypore strains bacterial blocks.

[0074] The preparation method of the Chinese mandarin tree extract comprises: weighing a certain amount of Chinese mandarin plant leaves, pulverizing them into 40 meshes, first adding 60% ethanol aqueous solution which accounts for 10 times the weight of the Chinese mandarin plant leaves, and extracting at 70°C After 1.5 hours, the supernatant was obtained after filtration, and the supernatant was concentrated and then vacuum freeze-dri...

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Abstract

The invention discloses a method for increasing the content of hyperoside in a phellinus igniarius liquid fermentation product. The method comprises the following steps: (1) preparing a phellinus igniarius seed liquid; (2) preparing a polyporus picipes karst seed liquid; (3) firstly inoculating the polyporus picipes karst seed liquid accounting for 1%-10% of the volume of the fermentation culture medium to a fermentation culture medium, culturing for 1-4 days at a temperature of 22 to 30 DEG C, next, regulating the temperature to 25 to 29 DEG C, inoculating the phellinus igniarius seed liquid accounting for 5%-20% of the volume of the fermentation culture medium to the fermentation culture medium and then further culturing for 2-5 days, thereby finally obtaining the phellinus igniarius liquid fermentation product after fermentation is terminated. According to the characteristics of the anabolic pathway of the liquid fermentation product hyperoside of the medical fungus phellinus igniarius, the method for increasing the content of the hyperoside in the phellinus igniarius liquid fermentation product is capable of greatly improving the content of the hyperoside in the phellinus igniarius mycelium by firstly generating an intermediate product by growing the polyporus picipes karst by use of a carbon source, and then adding the phellinus igniarius liquid strain and converting the intermediate product generated by the polyporus picipes karst into the desired metabolic end product by virtue of the liquid fermentation of the phellinus igniarius.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and in particular relates to a method for increasing the content of hyperin in a liquid fermented product of Phellinus phellifolia. Background technique [0002] Phellinus igniarius belongs to Basidiomycotina, Phellinus class, Rustiaceae, Pinifera. Phellinus extract has significant effects in the clinical application of inhibiting cancer cell metastasis and preventing cancer recurrence after surgery. It is currently a medicinal fungus with the highest efficiency among internationally recognized biological cancer treatment agents. [0003] Due to the particularity and complexity of the physiological state and the constraints of the external environment, Phellinus seldom forms fruiting bodies in nature, especially it takes many years to form usable fruiting bodies. Moreover, artificial cultivation is extremely difficult, the cultivation conditions are harsh, and the gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/60C12R1/645
Inventor 程俊文贺亮魏海龙胡传久付立忠李海波
Owner ZHEJIANG FORESTRY ACAD
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