49results about How to "Suitable for growth and reproduction" patented technology

The invention belongs to the technical fields of waste recycling and animal feed preparation and particularly discloses a tenebrio molitor and hermetia illucens feed based on organic refuse fermentation residues. The feed is prepared from, by mass, 30-40% of kitchen waste fermentation residues, 10-35% of agriculture and forestry waste fermentation residues, 8-25% of wheat bran, 5-15% of soybean meal, 4-6% of sweet potato vine powder, 1.5-5% of purslane, 0.5-3% of honeysuckle powder, 0.5-3% of multivitamins for feeding and 0.1-5% of bacillus subtilis probiotics. The feed uses the organic waste fermentation residues as main raw materials, is supplemented by Chinese herbal medicines and the probiotics, is more suitable for growth and propagation of tenebrio molitor and hermetia illucens larvae and is suitable for industrialized production, the preparation materials are simple and easy to obtain, and the problem of treatment of the organic waste fermentation residues is solved.

The invention relates to a method and equipment for dewatering municipal sludge with high dryness. The method comprises the following steps: putting to-be-treated sludge in a sludge treatment machine, and mixing and stirring with ferric sulfate or polymerized ferric sulfate; adding quick lime, and keeping on mixing and stirring to obtain treated sludge having neutral pH; and starting a feed pump of a filter press, pumping the treated sludge in the high-pressure diaphragm frame filter press, squeezing to obtain dewatered sludge cake, opening a filter plate, and delivering the dewatered sludge cake on a belt conveyer to the outside. The equipment comprises the sludge treatment machine, a sludge inlet pipe, the feed pump of the filter press, the high-pressure diaphragm frame filter press, a squeezing water pump, a squeezing water tank and the belt conveyor. According to the method, the dewatered sludge cake has a water content of less than 60 percent, has good ventilation after being ground, is suitable for growth and reproduction of microorganisms, can be directly subjected to aerobic fermentation, can be used for directly realizing reduction, harmlessness and resource of sludge, and is beneficial to sustained development.

The invention relates to a biomembrane carrier material, and specifically relates to a modified alveolar stone doped bioactive polylactic acid-polypropylene composite fiber carrier material and a preparation method thereof. The conventional pure inorganic and organic carriers are mixed to prepare the composite material; the fiber silk made of polymer materials such as polypropylene, polylactic acid, and the like has a better compatibility with microbes and good hydrophilicity. Moreover, the doped modified alveolar stone has a uniform and efficient adsorption performance. The added colloid graphite powder improves the mechanical performance of fiber silk and improves the adsorption performance of carrier on microbes. The fiber bundle is soaked in composite silica gel, after the treatment, the fiber bundle can adhere to a carbon source in fibers, and the growth of microbes is accelerated. The provided composite fiber carrier material has the advantages of light weight, large activated surface area, high bioactivity, and suitability for microbial growth and propagation and is capable of forming a film in a short time. The filling material made of the composite fiber carrier material has the advantages of good fluidity, difficulty in clustering, and aging resistant property, and is capable of improving the water purification efficiency.

The invention discloses a biological desulfurizing and deodorizing trickling-filtration tower device and a biological desulfurizing and deodorizing method for odor. The biological desulfurizing and deodorizing trickling-filtration tower device comprises a biological oxidizing tower, a chemical-absorption desulfurizing and deodorizing tower and a circular spraying device, wherein the biological oxidizing tower is formed by connecting a plurality of biological oxidizing units containing fillers and an odor-inflow cushioning area; a biological trickling filter is arranged at the top part of the tower, and an air inlet is arranged at the odor-inflow cushioning area; the chemical-absorption desulfurizing and deodorizing tower contains chemical absorbing liquid, an odor inlet is arranged at the bottom part of the chemical-absorption desulfurizing and deodorizing tower, and a rich-liquid inlet is arranged above the liquid level; the circular spraying device comprises a poor-liquid collecting groove, a rich-liquid collecting groove and a circulating pump; the rich-liquid collecting groove is used for collecting spraying liquid discharged from the biological oxidizing tower and conveying the spraying liquid to the rich-liquid inlet by the circulating pump; and the poor-liquid collecting groove is used for collecting chemical absorbing liquid discharged from the chemical-absorption desulfurizing and deodorizing tower and conveying the chemical absorbing liquid to the biological trickling filter. The invention also discloses a biological desulfurizing and deodorizing method utilizing the biological desulfurizing and deodorizing trickling-filtration tower device.

The invention relates to a method for ecology restoration and water quality maintenance of a city landscape water body by adopting reclaimed water as supply. According to the method, a composite type plant ecology system comprising two parts of an ecology bank revetment and a composite type ecology floating island is constructed, and an artificial regulation system is adopted to regulate a landscape water body, wherein the ecology bank revetment comprises a vegetation soil slope type bank revetment, a vegetation tile type bank revetment and a wetland type ecology bank revetment; bank revetments on both sides of the landscape water body are provided with multiple groups of composite type ecology floating islands, wherein each group of the composite type ecology floating islands is formed by connecting a plurality of floating island monomers, and the lower parts of each group of the composite type ecology floating islands are suspended with ecology grasses; the artificial regulation system comprises three parts of water supply regulation, water body disturbance / oxygen increase regulation and aquatic animal regulation. The method of the present invention is applicable for landscape water bodies adopting reclaimed water as a supply water source, wherein the reclaimed water meets requirements of "The reuse of urban recycling water-Water quality standard for scenic environment use". With the method of the present invention, natural ecology system construction and artificial regulation are combined, self-purification capacity of water bodies can be effectively enhanced, and landscape function of water bodies can be maintained.

The invention discloses a drawer biofilter adopting a composite slow release carbon source as a filler. The drawer biofilter comprises a shell, a gas discharging opening, a water distribution unit, drawer units, a supporting layer and a water outlet, wherein the water distribution layer is used for distributing water to be treated to the drawer units, the drawer units are used for filtering the water to be treated, every drawer unit comprises a drawer and the composite slow release carbon filler contained in the drawer, the supporting layer comprises sand grains and a supporting plate and is arranged below the drawer units, the water outlet is arranged in the bottom of the shell and is used for discharging ammonia nitrogen adsorption treated water, the gas discharging opening is arranged at the top of the shell, is connected with the shell through a flange, and is used for discharging N2 in the reaction process. The composite slow release filler is obtained through blending and wrapping of a viscose material used as the supporting layer and agricultural wastes and adsorptive alkaline substances used as an adsorption layer, the integral body of the filler is cylindrical, granular or spherical in shape, the diameter is 3-6 mm, and the porosity is 50-60%.

The invention relates to a method for producing methane by carrying out co-digestion on pig manure and tea-oil tree shell. The method for producing the methane by carrying out co-digestion on the pig manure and tea-oil tree shell concretely comprises the steps of mixing the tea-oil tree shell serving as a raw material with the pig manure in different proportions in a controllable constant temperature anaerobic stainless steel fermentation tank at the high temperature of 50 DEG C in a semi-continuous anaerobic fermentation way, and then carrying out co-digestion, thereby producing the methane. Compared with the pure pig manure fermentation, the method for producing the methane by carrying out the co-digestion on the pig manure and the tea-oil tree shell has the advantages that raw materials of the methane can be enriched, the problem of supply of the raw materials of the methane can be alleviated, popularization and application of the methane is promoted, and additional use value of tea-oil tree fruits is increased.

The invention discloses a sewage treatment agent. The sewage treatment agent is prepared from the following components in parts by weight: 50 parts of biological bacterium liquid, 90 to 130 parts of starch, 15 to 50 parts of polymeric silicate aluminum sulfate, 5 to 13 parts of polymeric ferric sulfate, 60 to 100 parts of activated sludge, 20 to 60 parts of plant ash, 18 to 36 parts of sodium acetate, 25 to 32 parts of polyacrylamide, 20 to 28 parts of ulexite, 20 to 30 parts of sodium bentonite, 15 to 33 parts of zeolite powder, 16 to 41 parts of coconut shell activated carbon and 3 to 19 parts of diatomite. The sewage treatment agent disclosed by the invention comprises a chemical treatment agent and a biological treatment agent, and the chemical treatment agent and the biological treatment agent have a mutual effect and are mutually matched, so that the sewage treatment effect is greatly improved.

The invention relates to a porcine epidemic diarrhea virus isolated culture method which comprises the following steps: thoroughly rinsing small intestine of a piglet, culturing the small intestine in a culture medium, inoculating, diluting a PEDV (Porcine Epidemic Diarrhea Virus) sample which is positive through detection into a suspension with the volume ratio of 1:1 by using normal saline, culturing and harvesting cells with the cytopathic effect (CPE), continuously freeze-thawing and inoculating single-layer vero cells, culturing by using the culture medium to obtain the cells with the cytopathic effect (CPE), namely, separating to obtain the porcine epidemic diarrhea virus. Compared with the prior art, the method is high in separation success rate and the separated porcine epidemic diarrhea virus is high in virulence and high in purity.

The invention relates to a deer ham and a curing method thereof. The curing method mainly comprises four steps of cooling and fresh-keeping, fractionated add of salt and curing, soaking, washing and basking, temperature controlling and fermentation of selected material. The lean meat of the cured deer ham is 65-75 percent by weight, the salt is 10-15 percent based on sodium chloride contained in the lean meat and the peroxide value is 20 percent meq / kg-32 percent meq / kg based on fat. The deer ham is characterized by reducing activity of active substances through cooling and fresh-keeping at low temperature, preventing residual blood contained in the meat from escape during the curing through fractionated curing and reserving the nutrition of deer meat to the utmost extent. Salt dosage, fermentation temperature and humidity conditions are controlled preferentially and growth and production of beneficial bacterium are facilitated while survival of harmful bacterium is inhibited, thus having advantages of complete fermentation of all parts and high content of a plurality of amino acids as well as a healthy and nutrient food with good taste, flavor and color.

The invention discloses a Hermetia illucens L feed and a preparation method thereof, wherein the Hermetia illucens L feed comprises kitchen waste, meat residue and Eichhornia crassipes. According to the present invention, the Hermetia illucens L feed efficiently utilizes the waste kitchen waste, the meat residue and the Eichhornia crassipes harmful to the environment, and is suitable for the growth and breeding of Hermetia illucens L larvae; the raw materials have advantages of low cost, simpleness and easy obtaining so as to solve the environment pollution problem of the kitchen waste and protect the environment; the fed Hermetia illucens L can be used as the high-quality animal protein and energy feed; the method is suitable for industrial production; and the Hermetia illucens L feed hasgood application prospects and market value.

The invention discloses a method for rainwater collection and water retention afforestation of arid mountain bare-rooted seedlings and belongs to the technical field of water-saving afforestation methods. The method comprises the steps of digging tree pits according to the size of the transplanted seedlings during afforestation, wherein soil which is dug out forms backfill; evenly mixing solid inoculant with part of backfill, and applying the mixture around root systems of the afforestation seedlings; field planting and watering. The solid inoculant is formed after an inoculant adsorption carrier is inoculated with liquid compound microorganism inoculant, wherein the liquid compound microorganism inoculant is formed by mixing of (Bacillus fusiformis) L106, (Brevundimonassp.) X60 and Lactobacillus casei, and the inoculant adsorption carrier is formed by adding crosslinked polyacrylamide to a mixture of mushroom residues, wormcast, saw dust and activated humic acid. Host materials of the inoculant adsorption carrier are suitable for growth and reproduction of microorganisms in complex inoculants, have high water-absorbing ability and can store more rainwater in the tree pits temporarily, and are suitable for survival of microorganisms in microorganism inoculants.

The invention discloses a solar-power diving crab-simulating robot with the functions of bionic observation and weeding. The robot comprises a main body, crab claws, crab legs and a mimic waterproof shell, wherein the crab legs and the crab claws are arranged on both sides of the main body separately, the crab claws include third claw arms which are fixed on both sides of the front part of the main body, second claw arms which are hinged to the end parts of the third claw arms and first claw arms which are hinged to the end parts of the second claw arms, and crab claw weeding shears are arranged at the tail ends of the first claw arms; and shear extension mechanisms and reset mechanisms are arranged inside the crab claws, the shear extension mechanism are used for driving the crab claw weeding shears to open and close and driving the crab claws to extend inwards at the same time, and the reset mechanisms are used for returning the crab claws to original positions. The disturbance to crabs is minimized, the influences on the growth and reproduction of the crabs are avoided, overgrowing aquatic weeds can be removed in time through the crab claw weeding shears, and it is ensured thatthe breeding environment is suitable for growth and reproduction of the crabs.

The invention discloses a making method of baijiu, comprising the steps of 1) crushing materials, to be specific, crushing 50-60 parts by weight of cereals to 20-25 mesh to obtain a raw material; 2) weighing distiller's yeast, to be specific, weighing 0.5-0.9 part by weight of distiller's yeast; 3) burdening, to be specific, mixing the raw material and the distiller's yeast in a closed container,adding 105-125 parts by weight of water and 4-8 parts by weight of soybean powder, adding auxiliaries into the closed container, and adjusting starch concentration; 4) fermenting, to be specific, performing anaerobic fermenting on the closed container at 18-25 DEG C; 5) distilling, to be specific, distilling the fermentation product, collecting steam from distillation, and cooling to obtain baijiu. The making method of baijiu has the advantages that fermenting time can be shortened and the technical problems of long fermenting time and low making efficiency are solved.

The invention discloses a power plant industrial wastewater treatment system and method, relates to the technical field of power plant industrial wastewater treatment, and aims at solving the problems that the sludge yield is large when wastewater is treated by an existing activated sludge method, more heavy metal ions exist in the power plant wastewater, the pH value is unbalanced, and then the generated sludge cannot be reutilized. One side of the upper end of an adjusting tank is provided with an industrial wastewater inlet, a coarse screen is mounted in the industrial wastewater inlet, a fine screen is mounted below the coarse screen, slag discharge valves are mounted on one side of the upper surface of the coarse screen and one side of the upper surface of the fine screen, two settling tanks are arranged at the lower end of the adjusting tank, a waste discharge valve is installed at the lower end of the sedimentation tank, a waste discharge pipe is installed at the lower end of the waste discharge valve, a primary sedimentation tank is arranged on one side of the adjusting tank, a first mud valve is installed at the lower end of the primary sedimentation tank, and a mud discharge pipe is installed at the lower end of the first mud valve.

The invention relates to a sugarcane seedling dipping agent, comprising the following raw materials in parts by weight: 40-53 parts of slaked lime, 5-7 parts of potassium fulvic acid, 6-8 parts of potassium sulphate, 15-18 parts of diammoniium sulfate, 2-3 parts of ferrous sulfate, 3-5 parts of humus, 12-14 parts of nitro humic acid and 4-5 parts of rare earth. Compared with prior art, the sugarcane seedling dipping agent is capable of sterilizing and disinfecting sugarcane seedlings, preventing diseases and supplementing sufficient nutrients, and is capable of improving absorption of sugarcane on nutrients, and quickening growth of sugarcane seedling stems.

The invention discloses distributed rural domestic sewage low-energy-consumption integrated treatment equipment, and belongs to the technical field of water treatment. The equipment comprises an integrated box-shaped main body, wherein the interior of the integrated box-shaped main body is sequentially divided into an anoxic tank, an aerobic tank, a sedimentation tank and an equipment room by separation walls along a sewage flowing direction, the anoxic tank and the aerobic tank are filled with biological fillers, the nitrification liquid in the aerobic tank flows back to the anoxic tank in agas stripping manner so as to achieve the denitrification function, the active biological membrane in the aerobic tank degrades COD, ammonia nitrogen, total phosphorus and other pollutants while utilizes oxygen, mud-water separation is achieved through a structure form forming by matching the mud bucket plate with holes of the sedimentation tank and a mud baffle and an internal inclined pipe, supernatant is discharged through an effluent weir, and effluent can reach the first-level B standard of Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant. A purpose of the invention is to provide distributed rural domestic sewage low-energy-consumption integrated treatment equipment having characteristics of simple and compact structure, small occupation area, low energy consumption and high treatment efficiency and used for sewage treatment.

The invention discloses an air freshener with sterilizing effect, prepared from Radix Oplopanacis Elati, Herba Gynostemmatis Pentaphylli, Herba Sedi, Rhizoma Smilacis Chinensis, Rhizoma Polygoni Cuspidati, Radix Pulsatillae, Herba Rabdosiae Graucocalycis, Herba Lysimachiae Foenum-graeci, Radix Aristolochiae, Radix Glycyrrhizae, sugarcane juice, EM original solution and the like; Radix Oplopanacis Elati and Herba Gynostemmatis Pentaphylli are put in important use and are functional in tonifying qi and supporting yang, adjusting immunity and resisting oxidation, so that they are capable of greatly improving human body resistance to germs and viruses; all Herba Sedi, Rhizoma Smilacis Chinensis, Rhizoma Polygoni Cuspidati and Radix Pulsatillae have the resistance-strengthening and evil-eliminating effects of clearing heat and toxicity, dispelling the wind and removing dampness, activating blood to remove stasis, benefiting blood and qi, soothing liver and reinforcing liver, are also functional in sterilizing, detoxifying and eliminating carbuncle, and accordingly are very suitable for acting as sterilizing preparations; the air freshener with sterilizing effect has a wide range of uses, can kill germs and freshen air, and also has the fragrant and refreshing effects; the air freshener with sterilizing effect provides board-spectrum killing for most germs, fungi and viruses, and can prevent and treat diseases.

The invention discloses a domestic sewage treatment agent. The domestic sewage treatment agent comprises, by weight, 50 parts of biological bacterial liquid, 90-130 parts of starch, 15-50 parts of polysilicate aluminum sulfate, 5-13 parts of polymeric ferric sulfate, 60-100 parts of activated sludge, 20-60 parts of plant ash, 18-36 parts of sodium acetate, 25-32 parts of polyacrylamide, 20-28 parts of natroborocalcite, 20-30 parts of sodium bentonite, 90-120 parts of plant fibers and 20-30 parts of aluminum chloride, wherein the plant fibers are prepared by smashing one or more of roots, stems and leaves of plants and then carrying out acid treatment and alkali treatment in sequence. The domestic sewage treatment agent comprises a chemical treatment agent and a biological treatment agent which mutually act on each other and are matched with each other, so that the sewage treatment effect is greatly improved.

The invention discloses a three-dimensional microelectrolysis bio-contact oxidation water purification system, which includes a sewage inlet pipe and one or more sewage electrolytic purification treatment tanks or sewage electrolytic purification treatment chambers. The sewage electrolytic purification treatment tank or sewage electrolytic purification treatment chamber is internally provided with a cathode electrode, an anode electrode, an upper grid, a lower grid and a biocarrier filler. The upper grid and the lower grid are respectively arranged on the inner wall of the sewage electrolytic purification treatment tank or sewage electrolytic purification treatment chamber. The biocarrier filler is arranged between the upper grid and the lower grid. The cathode electrode is disposed over the upper grid, and the anode electrode is disposed below the lower grid. The three-dimensional microelectrolysis bio-contact oxidation water purification system provided in the invention can improve the biodegradability of sewage, enhance the dissolved oxygen of water, strengthen the biological denitrification ability, form a biological chain suitable for decomposition of a plurality of organic pollutants in water, improve the sewage treatment efficiency, save investment cost, and has high and stable water quality purification standards.

The invention discloses a culture medium suitable for industrial production of bifidobacteria and a preparation method thereof. The culture medium consists of the following components: 55 to 63 grams per liter of corn steep liquor dry powder (containing 40 percent of protein), 5 to 10 grams per liter of glucose, and 0 to 1 gram per liter of tween-80, wherein the initial pH is between 6.5 and 7.2.The bifidobacteria BBMN68 and BB12 grow well in the culture medium, and the maximum amount of growth reaches 10<9> cfu / ml which is better than that of in the bifidobacteria culture media MRS and NPNL. The culture medium has low cost (the price is reduced by 95 percent compared with that of the NPNL culture medium and is reduced by 80 percent compared with that of the MRS culture medium) and simple preparation process, and is suitable for industrial mass production of the bifidobacteria.

The invention discloses an organic long-lasting slow-released fertilizer and a manufacturing method. The organic long-lasting slow-released fertilizer comprises the following components in parts by weight: 85-83 parts of a nitrogenous fertilizer, 7-5 parts of diethylenetriamine, 7-5 parts of humic acid, 4-1 parts of modified starch, 3-5 parts of polyving akohol and 2-1 parts of diethyl aminoethyl hexanoate DA-6.

The invention discloses a long-distance water taking dynamic testing system. The long-distance water taking dynamic testing system comprises a testing device, a liquid adding device, a detecting system and a supporting structure, the testing device comprises a water inlet, a water outlet and several testing pipes, the testing pipes are in series connection with one another, an inlet of the first testing pipe is connected with the water inlet through a liquid pipe, and an outlet of the last testing pipe is connected with the water outlet through the liquid pipe; the liquid adding device is arranged on the liquid pipe and is used for adding testing liquid for the testing device, and the detecting system is used for detecting the liquid in the testing device; the supporting structure is arranged below the testing device and supports the testing device. Many testing pipes can be arranged, and different growth stages of polluted organisms are simulated; the testing liquid is regularly and quantitatively added into the testing system through the liquid adding device, the sea area organism density in a water source is simulated, a testing environment suitable for growth and breeding of the polluted organisms is created, and a pollution change mechanism of the polluted organisms in a long-distance water taking system can be better studied.

The invention discloses a broad-spectrum antibacterial traditional Chinese medicine disinfectant and a preparation method. The disinfectant is prepared by adopting the following raw materials: Chinese ladiestresses roots, rhizoma polygonati, sweet tea vines, bryophyllum, mentha piperita, rhizoma picrorhizae, Chinese violet, creeping oxalis, cauliflower, lysimachia sikokiana, angelica root, sugar cane juice and EM raw dew. Chinese ladiestresses roots can tonify qi and yin, is rich in biological substances beneficial to a human body and has a function of preventing and resisting diseases; the rhizoma polygonati can tonify spleen and qi and nourish kidney, has the function of resisting pathogenic microorganism and various bacteria, can affect immunity and cyclic nucleotide content and has the function of increasing study and memory capacity; rich polysaccharide ingredients of rhizoma polygonati are mixed with EM raw dew and brown sugar, so that the EM raw dew beneficial bacteria can grow better and quicker; the antiseptic volume is increased; the product quality is increased; the unexpected technical effect can be achieved.

The invention provides an artificial mangrove wetland system for deep treatment of sewage, and the artificial mangrove wetland system comprises a sedimentation tank, a horizontal subsurface flow wetland bed, an oxygen-increasing wetland bed, a vertical flow wetland bed and a horizontal surface flow wetland bed which are sequentially connected. The sedimentation tank is filled with nano silica gravel. The bottoms of the horizontal subsurface flow wetland bed and the vertical flow wetland bed are both provided with a filler layer and a sea sand layer, the filler layer comprises a nano-silica gravel layer, the sea sand layer is positioned above the nano-silica gravel layer, and mangrove plants are planted in the horizontal subsurface flow wetland bed and the vertical flow wetland bed. The wall of the oxygen-increasing wetland bed is a honeycomb-shaped rough surface, and the honeycomb-shaped rough surface is provided with gravel blocks containing nano silicon oxide. An S-shaped dam is arranged in the horizontal surface flow wetland bed. By adopting the artificial mangrove wetland system, the removal efficiency of pollutants such as nitrogen and phosphorus in a water body is increased,the removal efficiency of the nitrogen and the phosphorus and the degradation efficiency of toxic and harmful substances are further improved, and the deep purification efficiency of the sewage is ensured.

The invention discloses a method for preparing a microbial fertilizer promoting morchella growth by spent mushroom compost of lentinus edodes. The microbial fertilizer comprises the spent mushroom compost of lentinus edodes, cow dung, humic acid, bean pulp, amino acid, medium trace elements and compound microorganism preparations. Fermentation is performed by two steps, primary fermentation includes that fresh organic fertilizers are fermented in a special fermentation bin GALLS or fermentation tank under aerobic condition and under the action of microorganisms. Secondary fermentation includesthat fermented finished products are stacked, the compound microorganism preparations are added to continue decomposing incompletely decomposed organic substances, the organic substances are changedinto stable humic substances, soil beneficial viable bacteria can be sufficiently activated by the microbial fertilizer, soil fertility is improved, good growth environments are provided for morchellaserving as autochthonic saprophytic bacteria, infectious microbes are inhibited, morchella growth is promoted, and yield and quality are improved. The method is a beneficial way for turning waste into wealth, resources of the spent mushroom compost of lentinus edodes are huge in China, environmental problems caused by stacking of the spent mushroom compost of lentinus edodes result in pollution,and the problems of bacteria-forestry contradiction and mushroom residue pollution can be effectively solved.

The invention discloses a method for preparing porcine circovirus through basket reactor fermentation. The method for preparing the porcine circovirus through the basket reactor fermentation is characterized by comprising the following steps: (1), preparing the host cell species; (2), preparing a basket reactor before inoculation; (3), performing host cell propagating culture; and (4), performingvirus proliferating culture: emptying cell growing liquid in the reactor, supplementing cell maintaining liquid with the volume equal to that of the emptied cell growing liquid, inoculating the porcine circovirus, 36-48h after inoculation, harvesting a virus solution for the first time, after harvesting the virus solution, adding the cell maintaining liquid with the volume equal to that of the harvested virus solution into the reactor, propagating, reproducing, and harvesting the virus again, circularly repeating for multiple times until the sugar consumption of cells in the virus solution tobe harvested is detected to be reduced down to 0.5g / L / day, and harvesting the last porcine circovirus solution in the batch. The method is simple and feasible; and by the method, the production cost can be greatly reduced, the unit titer of a porcine circovirus antigen is significantly improved, and the harvest of the virus solution antigen in each fermentation batch is increased.

The invention discloses a traditional Chinese medicine disinfectant and a preparing method. The traditional Chinese medicine disinfectant is prepared form fructus lycii, peppermint leaves, typhae pollen, all-grass of sedum sarmentosum, ehizomes of rodgersia aesculifolia, meadowrue roots and rhizomes, leaves of purpleflower holly, origanum vulgare L., Yunnan wintergreen stems and leaves, radix angelicae, sugar cane juice, EM original liquid and the like. Fructus lycii capable of tonifying the kidneys and nourishing the liver can influence the immunity and has the effects of remarkably increasing the number of leukocytes for in-vivo leucopenia, and thus the antibacterial capability of the human body is improved. The traditional Chinese medicine disinfectant contains rich various polysaccharide substances and amino, and is beneficial to health. More EM in the EM original liquid grows better and faster under the various kinds of sufficient sugar of fructus lycii and brown sugar, the disinfecting and sterilizing effects are improved, and the unexpected technical effect is achieved. All the medicine materials are in compatibility and form the traditional Chinese medicine disinfectant free of corrosion, pollution, irritation and harm to the human body, articles and the environment, and the traditional Chinese medicine disinfectant has high broad spectrum antibacterial and antivirus activity, has wide caring using value and has the effects of disinfecting, sterilizing and air refreshing.

The invention discloses rice tryptophan decarboxylase and a production method thereof, and belongs to the technical field of bioengineering. The nucleotide sequence of gene TDC for coding the rice tryptophan decarboxylase is as shown in SEQ ID NO. 1. The production method comprises the following steps of connecting the gene TDC as shown in SEQ ID NO.1 into a plasmid pEGX-4t-J to obtain a recombinant expression vector; introducing the obtained recombinant expression vector into escherichia coli to obtain recombinant bacteria; inoculating the recombinant bacteria into a culture medium for fermentation culture to obtain a fermentation culture solution; adding an inducer into the fermentation culture solution for induced culture to obtain an induced culture; and separating and purifying the induced culture to obtain the rice tryptophan decarboxylase. The rice tryptophan decarboxylase produced by the method is high in expression level and good in catalytic activity.

The invention discloses a rice tryptophan decarboxylase and a production method thereof, belonging to the technical field of bioengineering. The nucleotide sequence of the gene TDC encoding rice tryptophan decarboxylase is shown in SEQ ID NO.1. Connect the gene TDC shown in SEQ ID NO.1 into the plasmid pEGX-4t-J to obtain a recombinant expression vector; then introduce the obtained recombinant expression vector into Escherichia coli to obtain recombinant bacteria; inoculate the recombinant bacteria into the medium for fermentation culturing to obtain a fermentation culture liquid; adding an inducer to the fermentation culture liquid for induction culture to obtain an induced culture; separating and purifying the induced culture to prepare rice tryptophan decarboxylase. The rice tryptophan decarboxylase produced by the invention has high expression level and good catalytic activity.