The invention discloses a method for preparing
cell microvesicles based on
shear stress stimulation, and belongs to the technical field of bioengineering. The method comprises the following preparation steps of: taking cells, adding the cells into a culture solution, culturing, carrying out
shear stress treatment after the cells grow to 90%, collecting the cells, whirling, centrifuging, taking supernatant, and centrifuging again to obtain precipitate; and putting the precipitate into
phosphate buffered
saline, blowing, beating, uniformly mixing, centrifuging, taking supernatant, centrifuging again to obtain secondary precipitate, adding water, carrying out hypotonic treatment, and centrifuging to obtain the
cell microvesicles. According to the method for preparing the
cell microvesicles based on
shear stress stimulation, EVs with high content can be conveniently and quickly obtained; meanwhile, in the preparation process, chemical
reagent stimulation is not needed, heterogeneous
polymer particles cannot be generated, and manpower,
material resources and financial resources are saved; and the prepared cell microvesicles are uniform in size, the average particle size is 231nm, and the method has popularization and application values in the field of
biological materials.