The invention belongs to the field of skincare products, in particular to an anti-aging skincare product containing epidermal stem cell secretin and a preparation method of the anti-aging skincare product. The skincare product is prepared from the following components by weight percentage: 0.000001-0.0005% of epidermal stem cell secretin, 2-8% of plant extract, 1-2% of squalane, 0.10-0.25% of EDTA-2Na, 5-10% of glycerol, 0.05-1% of poly-glutamic acid, 0.06-0.12% of K350, 2-6% of isopropyl myristate, 0.5-2% of cetearyl alcohol, 1-2% of polydimethylsiloxane and the balance of water, wherein the plant extract is prepared from semen adenantherae pavoninae, edgeworthia chrysantha, radix acanthopanacis senticosi, ilicis rotundae cortex and fructus rubi. The skincare product provided by the invention can significantly improve skin aging, delay aging, reduce the incidence of adverse reactions, is simple in preparation method and stable in property.