Narrow-distributed hydroxyethyl starch and application thereof

A hydroxyethyl starch, narrow distribution technology, used in the field of medicine, can solve the problems of cell toxicity, affecting cell activity, and poor cell status.

Active Publication Date: 2017-05-31
WUHAN HUST LIFE SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the traditional cell separation liquid is toxic to the cells, and the state of the separated cells is not good, which affects the activity of the cells, which is not conducive to the subsequent cultivation of the extracted cells and the application of clinical treatment.

Method used

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  • Narrow-distributed hydroxyethyl starch and application thereof
  • Narrow-distributed hydroxyethyl starch and application thereof
  • Narrow-distributed hydroxyethyl starch and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prepare a cornstarch suspension with a concentration of 15% (w / v), gelatinize at 95°C for 30min, adjust the acidity of the system to 0.5mol / L with hydrochloric acid, set the temperature at 60°C, and react for 4 hours to obtain starch hydrolyzate; starch hydrolyzate The concentrated solution is obtained by separating the ultrafiltration membrane with a molecular weight cut-off of 50,000Da; 2 Carry out hydroxyethyl substitution reaction with oxirane under protection under sodium hydroxide catalysis, wherein starch hydrolyzate: sodium hydroxide: the reaction molar ratio of chloroethanol is 1:0.8:0.5, and the reaction time is 10 hours, and reaction temperature The temperature is 35°C; the reaction solution is decolorized by activated carbon, filtered and then spray-dried to obtain a powder product of narrowly distributed hydroxyethyl starch. According to the method of Ph.Eur.2.2.30and monograph (01 / 2011:1785), the weight-average molecular mass Mw of the product is 2.883e+5,...

Embodiment 2

[0031] Taking the commercially available Ficoll-sodium diatrizoate separating liquid as the control sample, respectively, with the narrow distribution hydroxyethyl starch, hydroxyethyl starch 130 / 0.4, hydroxyethyl starch 200 / 0.5 and hydroxyethyl starch 480 / The medicine of 0.7 is a test sample.

[0032] (i) Take fresh anticoagulated blood, carefully add it to the upper layer of Ficoll-sodium diatrizoate separation solution according to the volume 1:1, and centrifuge at 500g for 30 minutes at room temperature (18-22°C). Cells are divided into four layers, such as figure 2 shown. The first layer is the plasma layer (containing platelets). The second layer is a ring of opalescent lymphocytes (monocyte / mononuclear cell layer). The third layer is a transparent separation liquid layer. The fourth layer is the red blood cell layer (containing a large number of neutrophils). Collect the cells and put them into a test tube containing 10 mL of washing solution, mix thoroughly, and...

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Abstract

The invention discloses narrow-distributed hydroxyethyl starch and application thereof. The weight-average molecular weight Mw of the narrow-distributed hydroxyethyl starch is 250000-400000; the molar substitution degree MS of the narrow-distributed hydroxyethyl starch is 0.3-0.7; the ratio of C2 to C6 is 4-12; the narrow-distributed hydroxyethyl starch can be used as a reagent of a medium for animal tissue or human body tissue density separation, and particularly a medium for blood density separation. By improving key molecular weight distribution of hydroxyethyl starch, such as the weight-average molecular weight Mw, the molar substitution degree MS and the ratio of C2 to C6, compared with the prior art, the narrow-distributed hydroxyethyl starch can be used as a novel medium for animal tissue or human body tissue density separation, so that the problems that a cell separation liquid has toxicity for cells, cell activity can be affected and the like can be effectively solved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and more specifically relates to a narrow-distribution hydroxyethyl starch and its application. The narrow-distribution hydroxyethyl starch can especially be applied in the medical field. Background technique [0002] Hydroxyethyl starch is obtained from the derivatization reaction of pullulan. Its chemical structure is similar to polysaccharides and glycogen in the body, and its side effects are smaller than plasma substitutes such as gum arabic and sodium alginate. Moreover, due to the existence of the hydroxyethyl group, on the one hand, it reduces the degradation rate of hydroxyethyl starch by amylase in the body, making it have a certain anti-shock effect; Solubility in order to prepare a solution of a certain concentration for clinical use. Therefore, hydroxyethyl starch solution is currently a commonly used plasma substitute in clinical practice. The volume expansion effect and blood th...

Claims

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Application Information

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IPC IPC(8): C08B31/12C12N5/071C12N5/078
CPCC08B31/12C12N5/0602C12N5/0634C12N5/0642C12N2509/00
Inventor 高旭阮健王芳柳威向犇朱玉婷吴子寒
Owner WUHAN HUST LIFE SCI & TECH
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