The invention discloses a cream enzymolysis composition. The composition is characterized by comprising, by weight, 0.3-0.8 part of acetic acid, 4-6 parts of butyric acid, 2-3 parts of caprylic acid, 6-8 parts of decylic acid, 4-7 parts of dodecanoic acid, 3-5 parts of myristic acid, 6-8 parts of palmitic acid, 0.1-0.2 part of s-methyl butyrate, 1-2 parts of ethyl lactate, 0.3-0.6 part of ethyl caprate, 1-3 parts of ethyl laurate, 1-3 parts of gamma-decalactone, 1-2.4 parts of gamma-onalactone, 6-8 parts of delta-decalactone, 1-3 parts of gamma-unsecalactone, 0.3-1 part of butanedione, 1-2 parts of butanone alcohol, 6-8 parts of MHT, 3-4 parts of methyl-5-thiazoleethanol acetate, 2-3 parts of vanillin, 1-2 parts of ethyl vanillin and 20-50 parts of propylene glycol. According to the composition and a preparation method thereof, an alternative solution is provided for equipment and conditions required by producing natural enzymolysis cream products, the problem of unstable aroma of the natural enzymolysis cream products is solved, the production process is simple, the raw materials are easy to acquire, batch production can be achieved, requirements for mass production are satisfied, and the applicability is high.