The invention belongs to the technical field of
deep processing of marine products, and relates to a preparation method of
sea cucumber peptides, which comprises the following steps: (1)
grinding sea cucumbers into pulp; (2) uniformly mixing the
sea cucumber primary pulp with deionized water, heating until denaturation, cooling, and adding compound
protease for enzymolysis; (3) after the enzymolysis is finished, adding
activated carbon to remove
odor, then adjusting the pH value to 6.0-7.0, and heating to inactivate the
enzyme; (4) filtering by adopting an inorganic
nanofiltration ceramic membrane; (5)
freeze drying; and (6) preparing granules. The process is simple, the extraction rate is high, and the enzymolysis condition control and the improvement of the taste of the
sea cucumber peptides are facilitated; enzymolysis is thorough, the content of
small molecule peptides is high, the
peptide content in the product components reaches 80% or above; and the
peptide molecular
weight distribution result is that the content of peptides with a size of 1000Da or above is 6%, and the content of
small molecule peptides with a size of 1000Da or below is 94%. Through a biological enzymolysis technology, the sea cucumbers are hydrolyzed by biological enzymes to form active ingredients which are all soluble in water, the active ingredients are absorbed completely and rapidly, the content of small peptides in the product is high, and absorption and utilization of the active ingredients of the sea cucumbers are facilitated.