Preparation method of sea cucumber peptides

A technology of sea cucumber peptides and sea cucumbers, which is applied in the field of preparation of sea cucumber peptides, can solve problems such as hindering the deep development of products, high salinity in marine organisms, and affecting the appearance of products, so as to facilitate absorption and utilization, rich in nutrients, and health care and health preservation good effect

Inactive Publication Date: 2021-05-14
王朝辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most sea cucumber peptides are prepared by enzymatic hydrolysis. However, due to the high salt content and fishy smell of marine organisms, the removal process will increase a lot of cost and reduce the yield.
Moreover, the sea cucumber itself is brown or dark brown, and the color of its protease hydrolyzate is dark gray, which not only affects the appearance of the product, but also hinders the deep development of the product.

Method used

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  • Preparation method of sea cucumber peptides
  • Preparation method of sea cucumber peptides

Examples

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Effect test

Embodiment 1

[0023] A preparation method of sea cucumber peptide, comprising the following steps::

[0024] (1) Fresh sea cucumbers are cleaned of silt, removed from the viscera, cut into small pieces, and refined to obtain sea cucumber puree;

[0025] (2) Mix sea cucumber puree with deionized water at a ratio of 1:1, heat to 90°C and maintain for 15 minutes to moderately denature sea cucumber protein. After cooling, add soluble compound protease for enzymolysis. The compound protease consists of papain and trypsin Composition, the ratio is 2:1, the amount of compound protease added is 1000U, the enzymatic hydrolysis condition is pH8.0, the temperature is 35°C, and the time is 5h;

[0026] (3) After enzymatic hydrolysis, add 17% of the dry matter weight activated carbon to deodorize, then adjust the pH=6.0-7.0, heat up to sterilize the enzyme, then cool down to 73°C, and let it stand for 4 hours;

[0027] (4) The sea cucumber peptide clarified filtrate is filtered with an inorganic nanofi...

Embodiment 2

[0031] A preparation method of sea cucumber peptide, comprising the following steps::

[0032] (1) Fresh sea cucumbers are cleaned of silt, removed from the viscera, cut into small pieces, and refined to obtain sea cucumber puree;

[0033] (2) Mix sea cucumber puree with deionized water at a ratio of 1:1, heat to 95°C and maintain for 13 minutes to moderately denature sea cucumber protein. After cooling, add soluble compound protease for enzymolysis. The compound protease consists of papain and trypsin Composition, the ratio is 3:1, the amount of compound protease added is 700U, the enzymatic hydrolysis condition is pH8.0, the temperature is 35°C, and the time is 3h;

[0034] (3) After enzymatic hydrolysis, add 17% of the dry matter weight activated carbon to deodorize, then adjust the pH=6.0-7.0, heat up to sterilize the enzyme, then cool down to 74°C, and let it stand for 4 hours;

[0035] (4) The sea cucumber peptide clarified filtrate is filtered with an inorganic nanofil...

Embodiment 3

[0039] A preparation method of sea cucumber peptide, comprising the following steps:

[0040] (1) Fresh sea cucumbers are cleaned of silt, removed from the viscera, cut into small pieces, and refined to obtain sea cucumber puree;

[0041] (2) Mix sea cucumber puree with deionized water at a ratio of 1:1, heat to 100°C and maintain for 10 minutes to moderately denature sea cucumber protein. After cooling, add soluble compound protease for enzymolysis. The compound protease consists of papain and trypsin Composition, the ratio is 5:1, the amount of compound protease added is 1000U, the enzymatic hydrolysis condition is pH8.0, the temperature is 30°C, and the time is 5h;

[0042] (3) After enzymatic hydrolysis, add 17% of the dry matter weight activated carbon to deodorize, then adjust the pH to 6.0-7.0, heat up to sterilize the enzyme, then cool down to 75°C, and let it stand for 4 hours;

[0043] (4) The sea cucumber peptide clarified filtrate is filtered with an inorganic nan...

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Abstract

The invention belongs to the technical field of deep processing of marine products, and relates to a preparation method of sea cucumber peptides, which comprises the following steps: (1) grinding sea cucumbers into pulp; (2) uniformly mixing the sea cucumber primary pulp with deionized water, heating until denaturation, cooling, and adding compound protease for enzymolysis; (3) after the enzymolysis is finished, adding activated carbon to remove odor, then adjusting the pH value to 6.0-7.0, and heating to inactivate the enzyme; (4) filtering by adopting an inorganic nanofiltration ceramic membrane; (5) freeze drying; and (6) preparing granules. The process is simple, the extraction rate is high, and the enzymolysis condition control and the improvement of the taste of the sea cucumber peptides are facilitated; enzymolysis is thorough, the content of small molecule peptides is high, the peptide content in the product components reaches 80% or above; and the peptide molecular weight distribution result is that the content of peptides with a size of 1000Da or above is 6%, and the content of small molecule peptides with a size of 1000Da or below is 94%. Through a biological enzymolysis technology, the sea cucumbers are hydrolyzed by biological enzymes to form active ingredients which are all soluble in water, the active ingredients are absorbed completely and rapidly, the content of small peptides in the product is high, and absorption and utilization of the active ingredients of the sea cucumbers are facilitated.

Description

technical field [0001] The invention belongs to the technical field of deep processing of seafood, and in particular relates to a preparation method of sea cucumber peptide. Background technique [0002] Sea cucumber is a rare low-fat, low-sugar, cholesterol-free nutritious food in the world. It has been confirmed by modern medical and pharmacological research that sea cucumber contains more than 50 kinds of natural nutrients: high proportion of protein, 18 kinds of amino acids and no cholesterol, rich in calcium, phosphorus, iron, iodine, zinc, vanadium and other mineral elements, especially It contains sea cucumber mucopolysaccharides, sea cucumber saponins, sea cucumber lipids, chondroitin sulfate and other biologically active ingredients that have an important effect on lowering blood sugar and blood lipids. [0003] Bioactive peptides have a variety of human physiological regulation functions, such as improving immunity, hormone regulation, anti-oxidation, antibacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18C12P21/06C07K1/34C07K1/14
CPCA23V2002/00A23L33/18C07K1/14C07K1/34C12P21/06A23V2200/30A23V2250/55
Inventor 王朝辉
Owner 王朝辉
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