The invention relates to a method for efficiently extracting flavone from
ginkgo leaves and converting flavone into aglycon flavone.
Ginkgo leaves are washed clean and ground, the
ginkgo leaves are mixed with water, loaded into a
fermentation tank and sterilized under 121 DEG C, and
steam explosion is then carried out;
aspergillus niger is then added for
fermentation; mixed
enzyme solution is then added, the added amount is 2 to 5 percent of reaction substrate, and the time of reaction under the condition of 25 DEG C to 30 DEG C is 3 to 6 hours; Beta-
glucosidase is then added, the added amount is 1 percent of the reaction substrate, the reaction condition is that pH is 4.3 under the condition of 42 DEG C, and the reaction time is 5 hours; 1 percent of
protease is then added, and the time of reaction under the condition of 25 DEG C to 30 DEG C is 3 to 4 hours; temperature is increased to 95 DEG C for 10 minutes, extractant is then added to extract flavone, 12000g centrifuging is carried out for 20 minutes, supernatant is extracted and concentrated,
ethanol is then adopted for extraction, so that
ethanol eluent is obtained, the
ethanol eluent is concentrated, freeze-
drying is adopted, and thereby the product is obtained.