Method for efficiently extracting flavone from ginkgo leaves and converting flavone into aglycon flavone

A technology of ginkgo biloba and aglycone type, which is applied in the field of high-efficiency extraction of flavonoids and conversion into aglycon type flavonoids, which can solve the problems of anti-oxidation adverse effects, environmental pollution, and many by-products, achieve good extraction effect, and improve mass transfer characteristics , the effect of low extraction temperature

Inactive Publication Date: 2017-09-15
HUNAN UNIV OF SCI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods often shield the main functional group of flavonoids - the phenolic hydroxyl group, during the reaction process, which has adverse effects on antioxidants.
Simultaneously, these methods have many by-products, which easily cause environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Efficient extraction of flavonoids from Ginkgo biloba

[0028] Take 100kg of clean and dry ginkgo leaves, crush them into 20 meshes, add water to the ginkgo leaves, and add 50% of the water; put the mixture into a fermenter, sterilize at 121°C, and then carry out steam explosion, which uses saturated water vapor as the working medium , the pressure is 0.73MPa, and the holding time is 70 seconds; inoculate Aspergillus niger, the inoculation amount of 108 / mL seed solution is 100mL / 100kg, and the fermentation condition is 30°C for 3 days; then add mixed enzyme solution: cellulase: hemicellulose Enzyme: pectinase=2:1:5, the amount added is 2% of the reaction substrate, the reaction time is 6 hours at 30°C, then 1% protease is added, and the reaction time is 4 hours at 30°C; Raise the temperature to 95°C for 10 minutes, then add sodium citrate with a pH of 8.0 and 4%. The extraction conditions are as follows: 1:3 according to the weight of the ginkgo leaf and the e...

Embodiment 2

[0030] Example 2 Comparative Example 1

[0031] The composition and proportion of the extractant (calculated by weight): sodium bisulfite 10kg; tap water 8000kg; pH5.0.

[0032] Clean 300kg of dried ginkgo leaves, filter the water, put them into the extraction tank, add 5000kg of extractant at 94°C, cover and seal, raise the temperature in the extraction tank to 97°C, and keep 1 atmospheric pressure, stirring every half an hour For 1 minute, the temperature in the tank was controlled to drop by 2.5°C per hour. After leaching for 16 hours, 4400kg of the feed liquid was filtered out. Then add 3000kg of extractant solution to the extraction tank for the second leaching, filter out 3100kg of feed liquid after 10 hours, combine the two feed liquids with a centrifugal filter to coarsely filter out impurities below 20 mesh, and sand filter to fine filter out Impurities below 200 mesh were removed, and the feed liquid was cooled to 30°C.

[0033] The feed liquid flows through the NK...

Embodiment 3

[0034] Example 3 Comparative Example 2

[0035] (1) Pulverize 100 kilograms of ginkgo leaves with a pulverizer, pour them into an extraction tank, add 700 kilograms of water, and add 1.5 kilograms of calcium carbonate at the same time, heat, filter after boiling for 1.2 hours, and obtain a water extract; the same method Extract again, combine the first and second water extracts to obtain the combined solution;

[0036] (2) Concentrate the combined solution to 50L in a vacuum concentration tank at 65°C, add ethanol until the ethanol degree is 45%, stir fully, filter after standing for 3.5 hours, discard the alcohol sediment; dilute the filtrate with water, so that The ethanol degree is 18%, is adjusted to pH value 3.0 with dilute sulfuric acid, obtains dilute filtrate; The dilute filtrate is adsorbed with DM131 resin column, and resin column diameter height ratio is 1:5, flow velocity 1Bv / h, resin consumption is resin consumption is 50 kg (volume 50.1L);

[0037] (3) Adjust 1...

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PUM

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Abstract

The invention relates to a method for efficiently extracting flavone from ginkgo leaves and converting flavone into aglycon flavone. Ginkgo leaves are washed clean and ground, the ginkgo leaves are mixed with water, loaded into a fermentation tank and sterilized under 121 DEG C, and steam explosion is then carried out; aspergillus niger is then added for fermentation; mixed enzyme solution is then added, the added amount is 2 to 5 percent of reaction substrate, and the time of reaction under the condition of 25 DEG C to 30 DEG C is 3 to 6 hours; Beta-glucosidase is then added, the added amount is 1 percent of the reaction substrate, the reaction condition is that pH is 4.3 under the condition of 42 DEG C, and the reaction time is 5 hours; 1 percent of protease is then added, and the time of reaction under the condition of 25 DEG C to 30 DEG C is 3 to 4 hours; temperature is increased to 95 DEG C for 10 minutes, extractant is then added to extract flavone, 12000g centrifuging is carried out for 20 minutes, supernatant is extracted and concentrated, ethanol is then adopted for extraction, so that ethanol eluent is obtained, the ethanol eluent is concentrated, freeze-drying is adopted, and thereby the product is obtained.

Description

technical field [0001] The invention relates to the technical field of extraction of ginkgo flavonoids, in particular to a method for efficiently extracting flavonoids from ginkgo leaves and converting them into aglycon-type flavones. Background technique [0002] Ginkgo flavonoids are Ginkgo biloba extracts, which can increase cerebral blood flow, improve cerebral vascular circulation, protect brain cells, expand coronary arteries, prevent angina pectoris and myocardial infarction, prevent thrombosis, and improve the body's immunity. It is very beneficial to patients with coronary heart disease, angina pectoris, cerebral arteriosclerosis, senile dementia, and hypertension. However, ginkgolic acid is often adulterated in ginkgo biloba extract. Ginkgolic acid has potential sensitization and mutagenic effects, and strong cytotoxicity, which can cause severe allergic reactions, gene mutations, nerve damage, nausea and heartburn, anaphylactic shock, allergic purpura, exfoliativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16
CPCA61K36/16A61K2236/13A61K2236/15A61K2236/19A61K2236/333A61K2236/51A61K2236/53
Inventor 何福林袁志辉陈小明蒋琼凤赵昌会张瑞刘伟唐静杨芳
Owner HUNAN UNIV OF SCI & ENG
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