The present invention pertains to an antifouling coating composition, an antifouling coating film, a substrate having an antifouling coating film, an antifouling substrate, a method for producing a substrate having an antifouling coating film, and a method for preventing fouling of a substrate. The antifouling coating composition contains a hydrolyzable polymer (A) including at least one selected from the group consisting of polymers (A1) having structural units represented by formula (1) and polymers (A2) having structural units represented by formula (2), a tertiary carboxylic acid component (B) including versatic acid, and an antifouling component (C). In formula (1), M indicates zinc or copper, R1 independently indicate a hydrogen atom or methyl group. -COO-M-O-COR2 (2) In formula (2), M indicates zinc or copper, R2 indicates an organic group.