The invention discloses a self-emulsified nano-multi-
emulsion as well as a preparation method and application thereof, relating to the technical field of
medical treatment. The preparation method comprises the following steps: mixing a proper amount of Span 80 and polyoxyethylene hydrogenated
castor oil in a
mass ratio of 10 to 8, and mixing a proper amount of a mixture and a co-emulsifier in a
mass ratio of 8 to 2, so as to obtain a mixed surfactant; mixing 12-15 parts by weight of the mixed surfactant with 75 parts of an
oil phase, heating to 60 DEG C, maintaining the temperature, simultaneously carrying out magnetic stirring so as to completely dissolve the co-emulsifier in the mixed surfactant, adding a water phase at 60 DEG C, cooling to the
room temperature under a magnetic stirringstate, so as to obtain a W / O type nano-primary
emulsion; mixing a proper amount of polyoxyethylene hydrogenated
castor oil with the co-emulsifier in a
mass ratio of 8 to 2, so as to obtain a mixed emulsifier; and mixing a proper amount of the W / O type nano-primary
emulsion with the mixed emulsifier in
mass ratio of 8 to 2, so as to obtain the self-emulsified nano-multi-emulsion.