Self-emulsified nano-multi-emulsion as well as preparation method and application thereof

Abstract

The invention discloses a self-emulsified nano-multi-emulsion as well as a preparation method and application thereof, relating to the technical field of medical treatment. The preparation method comprises the following steps: mixing a proper amount of Span 80 and polyoxyethylene hydrogenated castor oil in a mass ratio of 10 to 8, and mixing a proper amount of a mixture and a co-emulsifier in a mass ratio of 8 to 2, so as to obtain a mixed surfactant; mixing 12-15 parts by weight of the mixed surfactant with 75 parts of an oil phase, heating to 60 DEG C, maintaining the temperature, simultaneously carrying out magnetic stirring so as to completely dissolve the co-emulsifier in the mixed surfactant, adding a water phase at 60 DEG C, cooling to the room temperature under a magnetic stirringstate, so as to obtain a W / O type nano-primary emulsion; mixing a proper amount of polyoxyethylene hydrogenated castor oil with the co-emulsifier in a mass ratio of 8 to 2, so as to obtain a mixed emulsifier; and mixing a proper amount of the W / O type nano-primary emulsion with the mixed emulsifier in mass ratio of 8 to 2, so as to obtain the self-emulsified nano-multi-emulsion.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a self-emulsifying nano compound emulsion and its preparation method and application. Background technique [0002] Despite the great prospects of small RNAs, the application of small RNAs for therapeutic purposes still faces major challenges: firstly, the "off-target" effect and immune stimulation hinder the application of small RNAs, how to minimize off-target effects and avoid immune stimulation becomes a major challenge. people's concerns. Through continuous research, it has been found that siRNA pool, chemical modification, and optimized siRNA design can significantly reduce off-target effects and immune stimulation; enable siRNA to be specifically delivered to target cells, reduce its contact with immune cells, internalization and endosomes Chances of medium retention can also reduce "off-target" effects and immune stimulation. The last and biggest challenge is the in vi...

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Problems solved by technology

[0009] For the defects existing in the prior art, the object of the present invention is to provide a kind of self-emulsifying nano compound emulsion and its preparation method and application, which solves the problem of biopharmaceutical classification system III (BCS III) (drugs with high solubility and low permeability, Such as protein, small nucleic acid and other active molecules) the problem of poor absorption and poor stability in the body

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