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48results about How to "Improve transfection efficiency" patented technology
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Delivery systems comprising biocompatible and bioerodable membranes
InactiveUS20040120979A1Reduce deliveryHigh expressionGenetic material ingredientsNanomedicineDendrimerIn vivo
The present invention relates to novel compositions and methods for delivering substances to target tissues and cells by contacting the targets with delivery systems associated with membranes (e.g., biocompatible or bioerodable membranes). More particularly, the present invention is directed to dendrimer-based methods and compositions for use in disease therapies, wound healing, and generally, improved gene transfection and compound delivery to target cells and tissues in vitro and in vivo.
Owner:ROESSLER BLAKE J +2
Superparamagnetic nanoparticles IN MEDICAL THERAPEUTICS and manufacturing method THEREOF
InactiveUS20110135577A1Safe and effectiveAvoid problemsBiocidePeptide/protein ingredientsDelivery vehicleMagnetite
The successful transfer of therapeutic agents such as genetic materials (e.g. nucleic acid) or drug into living cells is the most important issue depending on the development of the delivery carrier. A method for manufacturing superparamagnetic nanoparticles in medical therapeutics is described to develop nano-sized calcium phosphate (CaP) mineral was rendered magnetic as delivery vehicle. The CaP-based magnetized nanoparticles (NPs) were possessed superparamagnetic property by hetero-epitaxial growth of magnetite on the CaP crystallites and also showed no harm to the cultured cells and elicited no cytotoxicity. The magnetized CaP was demonstrated to have good plasmid DNA binding affinity or drug carrying capacity. It significantly increased the expression of gene transfection and efficiency in delivery to mesenchymal stem cells (MSCs) under exogenous magnetic field. According to the above facts, this newly-synthesized magnetized CaP NPs has great potential as a novel non-viral targeted delivery vehicle to be applied for medical applications.
Owner:NAT TAIWAN UNIV
Self assembling peptide systems and methods
InactiveUS20090098652A1Improve transfection efficiencyHigh efficiencyPolypeptide with localisation/targeting motifPeptide-nucleic acidsBiophysicsBiological activity
The present invention provides a self-assembling peptide system which utilizes a bioactive sequence which enhances transfection efficiency. In particular, the present invention provides compositions and methods for transfecting aggregates of cells at a higher efficiency.
Owner:NORTHWESTERN UNIV
Poloxamer and poloxamine compositions for nucleic acid delivery
InactiveUS20060013883A1Improvement in administrationImprove topical bioavailabilityPowder deliveryPeptide/protein ingredientsCell biologyPolymer
The invention features selected polymers such as poloxamer 188 (Pluronic® F68), poloxamer 237 (Pluronic® F87), poloxamer 401 (Pluronic® L121), poloxamer 338 (Pluronic® F108), Poloxamer 124 (Pluronic® L44), Poloxamer 184 (L-64), and poloxamines (Tetronics® 904, 908, 1107, and 90R4) that enhance expression from nucleic acids when administered into an organism, in particular to muscle, and further provides selected poloxamer formulations for delivery of nucleic acids to the liver.
Owner:GENETRONICS INC
Hollow mesoporous silicon dioxide nanoparticle, hollow mesoporous silicon dioxide nano-carrier and preparation method thereof
ActiveCN106698447AImprove transfection efficiencyLow toxicitySilicaInorganic non-active ingredientsIonNanoparticle
The invention relates to a hollow mesoporous silicon dioxide nanoparticle and a preparation method thereof. The preparation method comprises the following steps: magnetically stirring and mixing ethanol, deionized water and ammonia water at a certain temperature, adding ethyl orthosilicate for continuous reaction, adding premixed ethyl orthosilicate and trimethoxyoctadecylsilane for continuous reaction, vacuum drying an obtained mixture after being etched by sodium carbonate, calcining at 550 DEG C to obtain the hollow mesoporous silicon dioxide nanoparticle. The nanoparticle is dispersed in ultrapure water, and polymine is added to obtain a hollow mesoporous silicon dioxide nano-carrier after mixing; and the hollow mesoporous silicon dioxide nano-carrier is mixed with a gene to obtain a hollow mesoporous silicon dioxide gene nano-carrier. The hollow mesoporous silicon dioxide nano-carrier prepared by the preparation method disclosed by the invention has the advantages of good dispersity, high gene loading capacity and high transfection efficiency (twice of 25kDa PEI), and has a practical value of clinical application.
Owner:GUANGZHOU ZHONGDA NANSHA TECH INNOVATION IND PARK +1
Composite siRNA nanocarrier and preparation method and application thereof
ActiveCN109125291AHighly specific targetingImprove transfection efficiencyOrganic active ingredientsPharmaceutical non-active ingredientsNanocarriersSerum protein albumin
The invention discloses a composite siRNA nanocarrier, comprising exosome lipid membrane from autologous tumor cells and siRNA-carrying cationic serum albumin covered in the exosome lipid membrane. The invention also discloses a preparation method and application of the composite siRNA nanocarrier. The composite siRNA nanocarrier has the advantages of high transfection, specific targeting propertyof cancer metastatic tissues, good biocompatibility, good stability, zero immunogenicity and the like.
Owner:NANTONG UNIVERSITY
Ternary complex nanodrug, preparation method and application thereof in preparation of light controlled release nano-delivery system
ActiveCN108524946APromote endocytosisEnhanced nuclear intakeEnergy modified materialsGenetic material ingredientsTreatment effectPolymer degradation
The invention discloses a ternary complex nanodrug, a preparation method and application thereof in preparation of a light controlled release nano-delivery system. The ternary complex nanodrug comprises a thioketal bond crosslinked low molecular weight PEI-based polymer, a hyaluronic acid polymer attached with a photosensitizer, and a nucleic acid drug. The photosensitizer contained in the complexcan generate reactive oxygen species (ROS) under the control of exogenous red light, realize photochemical internalization (PCI) mediated lysosome escape and photopromoted polymer degradation, thus finally realizing the purposes of reducing the toxic and side effects of materials, improving the transfection efficiency, and enhancing the tumor gene therapeutic effect.
Owner:SUZHOU UNIV
Modified polyethyleneimine and application thereof in the preparation of gene transfection vector reagent
ActiveCN104974343ALow toxicityHigh gene transfection capacityOther foreign material introduction processesCell membraneComposite nanoparticles
The invention relates to the field of polymer modification, and in particular to a modified polyethyleneimine and application thereof in the preparation of a gene transfection vector reagent. According to the present invention, hydrophobic rosin acid or dehydroabietic acid is grafted onto polyethyleneimine to obtain the modified polyethyleneimine. The hydrophobic group of the modified polyethyleneimine forms a dense hydrophobic core with strong hydrophobicity due to the intermolecular forces in water, hydrophilic polyethyleneimine forms hydrophilic shell, thereby forming a nano micelles with shell-core structure, and nanoparticles with small particle size formed after DNA binding. The composite nanoparticles in smaller size gains tighter structure, so that composite nanoparticles are easier to pass through the cell membrane structure of a lipid bilayer, and the efficiency of transfection is improved. The modified polyethyleneimine can be applied to the preparation of gene transfection vectors and has the characteristics of high biological safety, high transfection efficiency, and low cost.
Owner:SOUTH CHINA AGRI UNIV
Gold nanoparticle-liposome composite nanoparticles, pharmaceutical composition and applications thereof
InactiveCN108498461AImprove transfection efficiencyImprove cell uptake and transfection efficiencyPeptide/protein ingredientsGenetic material ingredientsPeptideSurface modification
The present invention provides gold nanoparticle-liposome composite nanoparticles, which are gold nanoparticles encapsulated with a liposome vesicle, wherein the surface of the liposome vesicle is modified with distearoyl phosphatidylethanolamine-polyethylene glycol, the surface of the gold nanoparticles is modified with plasmid and a penetrating peptide, and the liposome vesicle comprises dioleoyl phosphoethanolamine, 2,3-dioleyloxypropyltrimethyl ammonium chloride and cholesterol. The invention further provides a preparation method of the gold nanoparticle-liposome composite nanoparticles ,and a pharmaceutical composition comprising the gold nanoparticle-liposome composite nanoparticles. According to the present invention, the gold nanoparticle-liposome composite nanoparticles as the carrier have the high transfection effect, and the pharmaceutical composition has good treatment effects on various tumors.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Modified polyethyleneimine derivative as well as synthesis method and application thereof
InactiveCN112142972AImprove transfection efficiencyGood gene delivery capability for local administrationPharmaceutical non-active ingredientsGene therapyCell EvaluationPerylene derivatives
The invention discloses a modified polyethyleneimine derivative as well as a synthesis method and application thereof. According to the invention, Triton X-100 is conjugated to polyethyleneimine, suchthat a PEI-Triton-N carrier is obtained. In-vitro cell evaluation shows that the carrier shows good safety and stability; cell transfection effect experiments show that the carrier shows efficient and low-toxicity gene delivery capacity, can significantly improve the gene transfection efficiency of various cells including skin immortalized cells HaCaT difficult to transfect, and is an excellent in-vitro transfection reagent; an in-vivo toxicity experiment shows that the carrier has no obvious systemic toxicity; and a gene delivery experiment result of mouse skin local administration shows that the carrier has good local administration gene delivery capability and is a good local administration gene delivery vector.
Owner:PEKING UNIV
Alpha helix cationic polypeptide as well as preparation method and application of alpha helix cationic polypeptide
ActiveCN108003343AEndocytosis into goodExcellent membrane penetrating activityPowder deliveryEnergy modified materialsDiseaseCancer cell
The invention provides an alpha helix cationic polypeptide as well as a preparation method and application of the alpha helix cationic polypeptide. The alpha helix cationic polypeptide takes a photosensitizer porphyrin as a core and alpha helix polypeptides as four arms, and a nucleotide drug prepared from the polymer can be applied to a nucleotide drug delivery system. The polymer provided by theinvention has good biocompatibility, low cytotoxicity and light activation ability. The polymer provided by the invention is self-assembled in a water solution to form nanoparticles which has good stability, biocompatibility and low cytotoxicity; the preparation method is simple and high in repeatability, and the polymer serving as a carrier is not only capable of protecting nucleic acid such asDNA from being degraded, but also capable of being combined with the scale effect of the nanoparticles so as to be used for treating diseases; and in addition, the transfection promoting effect for different cancer cells can be achieved by virtue of the light activation ability.
Owner:SUZHOU UNIV
Magnetic nanometer gene vector system as well as preparation method and application thereof
ActiveCN103255175AEasy to prepareFast preparation methodGenetic material ingredientsOther foreign material introduction processesMagnetite NanoparticlesGene vector
The invention discloses a magnetic nanometer gene vector system as well as a preparation method and an application thereof. The gene vector system comprises electronegative magnetic nanoparticles, positive-ion vectors and genes, wherein the three components are prepared into the magnetic nanometer gene vector system according to certain proportions and composite sequences through electrostatic interactions. The magnetic nanometer gene vector system disclosed by the invention is simple in preparation method and has physicochemical properties of small grain diameter and proper surface charge. Compared with non-magnetic nanometer composites, the magnetic nanometer gene vector system has the advantages that the transfection efficiency to cells of the system is remarkably improved under the action of an additional magnetic field; and meanwhile, the cytotoxicity of the system is further reduced.
Owner:SICHUAN UNIV
Compositions comprising a sirna and lipidic 4,5-disubstituted 2-deoxystreptamine ring aminoglycoside derivatives and uses thereof
InactiveUS20100035974A1Increase powerImprove transfection efficiencyOrganic active ingredientsSpecial deliveryGlycoside formationIn vivo
The present invention relates to a composition comprising a siRNA and a transfecting compound consisting of an aminoglycoside of the class of 4,5-disubstituted 2-deoxystreptamine ring linked via a spacer molecule to a lipid moiety of formula —(R1)R2 wherein R1 and R2 represent, independently of one another, a hydrogen atom or a fatty aliphatic chain or R1 or R2 is absent, with the proviso that at least one of R1 and R2 represents a fatty aliphatic chain; or —OR3 or —NR3 wherein R3 represents a steroidal derivative. The invention also concerns in vitro and in vivo applications of these compositions.
Owner:CENT NAT DE LA RECHERCHE SCI +1
Antiviral small nucleic acid and temperature-sensitive type gel preparation and application thereof
ActiveCN102899327AHigh mechanical strengthNot easy to collapseAerosol deliveryGenetic material ingredientsGeneticsFhit gene
The invention discloses an antiviral small nucleic acid and the application of the small nucleic acid in preparing a medicine for suppressing herpes simplex virus (HSV) and human papilloma virus (HPV). The small nucleic acid is a double strand nucleic acid, the positive-sense strand sequence is 5 in a sequence table, and the antisense strand sequence is 6 in the sequence table; the small nucleic acid can be prepared into a temperature-sensitive type gel preparation; the temperature-sensitive type gel preparation is injected (or sprayed) into irregular cavities such as vagina or mouth, the expression of host B2M genes in epithelial cells at the cavities and the cervix uteri and the expression of externalHPV E7 are suppressed, so that the antiviral small nucleic acid can be used for suppressing the infection of the HPV of the HSV-1 virus and / or for treating the precancerous lesions of uterine cervix caused by the HVP.
Owner:YOUJIA (HANGZHOU) BIOMEDICAL TECH CO LTD
Lipid compound, composition containing lipid compound and application
PendingCN114716355AEasy metabolic clearanceHigh transfection efficiencyPowder deliveryPharmaceutical non-active ingredientsPharmaceutical SubstancesTGE VACCINE
The invention relates to the technical field of biological medicine, and particularly discloses a lipid compound, a composition containing the same and application. The lipid compound has a structure as shown in a formula (I) or a formula (II). The lipid compound can be ionized into a cationic compound under an acidic condition, and the cationic compound is combined with negatively-charged active pharmaceutical ingredients through electrostatic interaction, so that drug-loaded lipid nanoparticles are assembled, and the active pharmaceutical ingredients are delivered. The lipid compound provided by the invention is simple in structure, simple in reaction path and high in yield, and the constructed drug-loaded lipid nanoparticles can be used for preparing nucleic acid drugs, gene vaccines, polypeptide or protein drugs and small molecule drugs, and have wide application prospects.
Owner:SOUTH CHINA UNIV OF TECH
Novel crosslinked pei nanoparticle based transfection agents for delivery of biomolecules
InactiveUS20100029750A1Reduced cytoxicityEfficient polymeric transfectingOrganic active ingredientsGenetic material ingredientsCross-linked polyethyleneBiophysics
The present invention relates to a novel crosslinked polyethylenimine (PEI) nanoparticle based nucleic acid transfection agent wherein the crosslinker is having carbon chain in the range of C2 to C8, ranging between 3.27-19.8%, having the size of nanoparticle ranging between 20-600 nm and zeta potential ranging from +5 to 50 mV.
Owner:COUNCIL OF SCI & IND RES
Method for transfecting exogenous nucleic acid by use of atmospheric pressure cold plasma jet
InactiveCN104372028AEasy transfectionEasy to operateOther foreign material introduction processesBiotechnologyQuartz
The invention discloses a method for transfecting an exogenous nucleic acid by use of atmospheric pressure cold plasma jet and belongs to the technical field of biology. The method comprises the following steps: 1) counting to-be-transfected cells, planting the cells in a porous cell culture plate, and adding a culture solution to obtain a to-be-transfected cell solution; 2) adjusting a plasma jet source device, introducing a basic gas, applying sinusoidal high voltage after the gas flow is stable, and keeping the voltage constant until bluish violet plasma jet is blown out from the bottom of a quartz tube; 3) treating the to-be-transfected cells for 5-60 seconds directly under the plasma jet source, moving away the cells and adding the to-be-transfected exogenous nucleic acid, mixing evenly and then putting the mixed solution back into an incubator for continuous culture. The method for transfecting the exogenous nucleic acid by use of the atmospheric pressure cold plasma jet is capable of conveniently transfecting the exogenous nucleic acid; after transfection, the cell mortality rate is relatively lower than those of methods by use of lipidosome, electrotransfection and the like. Besides, the transfection time is very short and the transfection can be completed within dozens of seconds.
Owner:XI AN JIAOTONG UNIV
PLNPs as well as pharmaceutical composition and application thereof
InactiveCN108096189AImprove transfection efficiencyGood treatment effectMacromolecular non-active ingredientsAntineoplastic agentsLipid formationLipid particle
The invention provides PLNPs. The PLNPs are nanoparticles wrapped by liposome vesicles, and the surfaces of the liposome vesicles are modified with distearoyl phosphatidylethanolamine-PEG; the nanoparticles are prepared from lipid particles, chondroitin sulfate and protamine; the liposome vesicles are prepared from dioleoyl phosphatidylethanolamine, N-[1-(2,3-dioleyloxy) propyl]-N,N,N-trimethylammonium chloride and cholesterol. The invention further provides a preparation method of the PLNPs and pharmaceutical composition containing the PLNPs. The PLNPs as a carrier have higher transfection effect. The pharmaceutical composition has better treatment effect on various tumors.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Device for ultrasound microbubble-mediated transfection
PendingCN108642089AImprove transfection efficiencyOther foreign material introduction processesUltrasonic sensorUltrasound contrast media
Owner:PEKING UNIV SHENZHEN HOSPITAL
Slow-virus vector system and preparation method thereof
InactiveCN102229963ALow costHigh transfection efficiencyGenetic engineeringFermentationVector systemShuttle plasmid
The invention relates to a slow-virus vector system and a preparation method thereof, belonging to the field of biotechnology. The slow-virus vector system comprises a shuttle plasmid CSII-EF-MCS-IRES2-Venus, a packaging plasmid pCAG-HIVgp and an envelop protein plasmid pVSV-G-RSV-Rev; a host cell is transfected by the shuttle plasmid CSII-EF-MCS-IRES2-Venus, the packaging plasmid pCAG-HIVgp and the envelop protein plasmid pVSV-G-RSV-Rev in the vector system, namely the plasmids are packaged in the host cell to obtain the slow-virus vector system. Compared with the prior art, the slow-virus vector system and the preparation method thereof provided by the invention have the advantages as follows: (1) the cost is low: by taking the transfection of cells in per 10 centimeters of 10 dishes asexample, the price of the recommended liposome 2000 with the volume of 600 microliters (60 microliters*10) is about 1500 yuan; however, the cost consumed by utilizing the method can be reduced to 0.62 yuan so that the cost can be greatly reduced; (2) the transfecting efficiency is high and the transfecting rate can be more than 80%; and (3) and the valence of the virus is high and can be 5.79*10<9>.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
A kind of poly(beta-amino ester) type polymer gene carrier containing disulfide bond and its synthesis method and application
ActiveCN106554499BPromote degradationLow toxicityGenetic material ingredientsGene therapyAmino estersSynthesis methods
Owner:NANJING UNIV OF SCI & TECH
Fusion mixture
InactiveCN105307637AImprove transfection efficiencyFused cellsLiposomal deliveryCell membraneMembrane cell
Owner:FORSCHUNGSZENTRUM JULICH GMBH
Nano-composite vector for transfection, nucleic acid nano-drug as well as preparation method and application of nano-composite vector and nucleic acid nano-drug
ActiveCN113262307AImprove transfection performanceStable structureOrganic active ingredientsPharmaceutical delivery mechanismDrug structureMolecular biology
Embodiments of the invention relate to a nano-composite vector for transfection, a nucleic acid nano-drug as well as a preparation method and application of the nano-composite vector and the nucleic acid nano-drug, belonging to the field of biomedicines. The nano-composite vector for transfection comprises a fullerene derivative and a high-molecular polymer with negative charges, wherein the fullerene derivative and the negatively-charged high-molecular polymer are combined through electrostatic interaction and pi-pi interaction, the zeta potential of the nano-composite vector formed by combination is positive; and the nano-composite vector and siRNA are combined through electrostatic interaction and pi-pi interaction to obtain the nucleic acid nano-drug. The nano-composite vector can load siRNA drugs, can assist siRNA in penetrating through cell barriers and improves the transfection performance of siRNA; and the formed nucleic acid nano-drug is stable in structure and good in biocompatibility, can effectively protect siRNA from being degraded by RNA enzyme, and achieves lung transfection of siRNA through respiratory tract inhalation drug delivery, and therefore, the expression of a target gene in a lung tissue can be effectively inhibited.
Owner:INST OF CHEM CHINESE ACAD OF SCI +1
SiRNA-loaded bionic ZIF-8 nanometer transmission system, and preparation method and application of siRNA-loaded bionic ZIF-8 nanometer transmission system
InactiveCN113786390AImprove transfection efficiencyGood treatment effectOrganic active ingredientsPowder deliveryTherapeutic effectTransfection
The invention provides an SiRNA-loaded bionic ZIF-8 nanometer transmission system, and a preparation method and application of the siRNA-loaded bionic ZIF-8 nanometer transmission system. The nanometer transmission system comprises ZIF-8 and siRNA wrapped by a cell membrane. The nanometer transmission system has a relatively high transfection effect, has a relatively good treatment effect for tumor inhibition, and can be prepared on a large scale.
Owner:GUANGZHOU MEDICAL UNIV
Magnetic transfection reagent based on supramolecule self-assembly
InactiveCN105295055AThe reaction mechanism is clearThe conditions are ripe for explorationOther foreign material introduction processesMagnetic sphereTransfection
The invention relates to a magnetic transfection reagent based on supramolecule self-assembly, wherein magnetic spheres prepared through a high temperature decomposition method and six-site fully-substituted alpha-cyclodextrin-PEI600 (CD-PEI) are assembled through self-assembly to form the magnetic transfection reagent. According to the present invention, the preparation method of the magnetic transfection reagent is provided, the structure of the magnetic transfection reagent is characterized, and the biological toxicity and the transfection performance of the magnetic transfection reagent at the cell level are characterized; the preparation method is mild, simple and green environmental protection; and the synthesized magnetic transfection reagent has characteristics of smaller particle size, surface having a lot of positive charges, good siRNA combination ability, low biological toxicity, high transfection efficiency, and good application prospects, and the performance of the magnetic transfection reagent as the siRNA vector is excellent.
Owner:NANKAI UNIV
Microfluidic systems and methods for lipoplex-mediated cell transfection
PendingUS20200324288A1High transfection efficiencySlow changeOrganic active ingredientsBioreactor/fermenter combinationsMembrane permeabilityCell membrane
Systems and methods for transfection using a microfluidic device are disclosed. Microdroplets encapsulate cells, transfection molecules, and cationic lipid transfection reagent. Droplet chaotic advection in a rendering channel of the system results in a uniform lipid-DNA complex (lipoplex) formation, which can improve gene delivery efficacy. The shear stress exerted on cell membranes during the chaotic mixing increases membrane permeability, which when combined with the co-confinement of cell and lipoplex, improves transfection efficiency of the cell. The systems and methods can be used for a variety of applications such as gene therapy, in vitro fertilization, regenerative medicine, cancer treatment, and vaccines.
Owner:PIONEER HI BRED INT INC +1
Antiviral small nucleic acid and temperature-sensitive type gel preparation and application thereof
ActiveCN102899327BImprove transfection efficiencyHigh mechanical strengthGenetic material ingredientsAerosol deliveryGel preparationTemperature sensitive
The invention discloses an antiviral small nucleic acid and the application of the small nucleic acid in preparing a medicine for suppressing herpes simplex virus (HSV) and human papilloma virus (HPV). The small nucleic acid is a double strand nucleic acid, the positive-sense strand sequence is 5 in a sequence table, and the antisense strand sequence is 6 in the sequence table; the small nucleic acid can be prepared into a temperature-sensitive type gel preparation; the temperature-sensitive type gel preparation is injected (or sprayed) into irregular cavities such as vagina or mouth, the expression of host B2M genes in epithelial cells at the cavities and the cervix uteri and the expression of externalHPV E7 are suppressed, so that the antiviral small nucleic acid can be used for suppressing the infection of the HPV of the HSV-1 virus and / or for treating the precancerous lesions of uterine cervix caused by the HVP.
Owner:YOUJIA (HANGZHOU) BIOMEDICAL TECH CO LTD
A preparation method of nano-sulfonated graphene and application of nano-sulfonated graphene as gene transfer material
ActiveCN103641106BLow costReduce manufacturing costGenetic material ingredientsGrapheneSulfanilic acidHuman breast
The invention relates to the technical field of gene transfer materials, particularly a preparation method of nano sulfonated graphene and application of nano sulfonated graphene as a gene transfer material. The preparation method of the nano sulfonated graphene comprises the following steps: 1. preparing graphene oxide; 2. preparing reduced graphene oxide; 3. preparing diazonium salt of sulfanilic acid; and 4. preparing the nano sulfonated graphene. The nano sulfonated graphene can be used as a gene transfer material since positive charges of ions on particle surfaces can be combined with green fluorescent proteins in a non-covalent mode to perform transfection; and when being used as a gene transfer material, the nano sulfonated graphene has the advantages of high transfection effect, favorable cell compatibility and high cell survival rate. The transfection efficiency of the nano sulfonated graphene in human breast cancer cells is up to 40% or so.
Owner:THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
High-molecular polymer for treating tumors and preparation method and application thereof
ActiveCN111303357APrecise transportationPayloadOrganic active ingredientsPharmaceutical non-active ingredientsLysosomeMedicine
The invention relates to a high-molecular polymer for treating tumors and a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: synthesizing PEGMA-(RAFT); synthesizing PEGMA-b-PDPA-(RAFT); synthesizing PEGMA-b-PDPA-b-PDMAEMA-(RAFT); desulfurization reaction; and synthesizing the high-molecular polymer ma< 1 >-PMDM. The invention also discloses the high-molecular polymer prepared by the preparation method and used for treating tumors and application of the high-molecular polymer. According to the preparation method, the high-molecular polymer for treating tumors can be prepared very efficiently; the high-molecular polymer can improve the stability of the carrier, improve the cellular uptake capacity and improve the transfection capacity, the composition and the structure of the carrier have the capacity of overcoming the lysosome biological barrier, and the high-molecular polymer can be applied to a targeting drug carrier for treating tumors.
Owner:GUIZHOU MINZU UNIV
Nanocomposite carrier for transfection, nucleic acid nanomedicine and preparation method and application thereof
ActiveCN113262307BImprove transfection efficiencyEfficient transportOrganic active ingredientsNanomedicineLung tissueBiocompatibility
Embodiments of the present invention relate to a nanocomposite carrier for transfection, nucleic acid nanomedicine, a preparation method and application thereof, and belong to the field of biomedicine. The nanocomposite carrier for transfection includes fullerene derivatives and negative The charged high molecular polymer, the fullerene derivative and the negatively charged high molecular polymer are combined through electrostatic interaction and π-π interaction, and the combined nanocomposite carrier has a positive zeta potential, The nanocomposite carrier is combined with siRNA through electrostatic interaction and π-π interaction to obtain nucleic acid nanomedicine. The nanocomposite carrier of the present invention can load siRNA drug, can assist siRNA to pass through cell barrier, improve the transfection performance of siRNA, the formed nucleic acid nanometer drug has stable structure and good biocompatibility, and can effectively protect siRNA from being damaged by RNase. It can be degraded, and the siRNA can be transfected into the lungs by inhalation administration, so that the expression of the target gene in the lung tissue can be effectively inhibited.
Owner:INST OF CHEM CHINESE ACAD OF SCI +1
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