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Gold nanoparticle-liposome composite nanoparticles, pharmaceutical composition and applications thereof

A composite nanoparticle, gold nanoparticle technology, applied in the field of medicine, can solve the problems of limited application, large size of Cas9 protein, delivery, etc., achieve good therapeutic effect, improve cell uptake rate and transfection efficiency, and high transfection effect. Effect

Inactive Publication Date: 2018-09-07
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large size of the commonly used Cas9 protein, it is difficult to efficiently deliver it with the current commonly used carriers, which limits its application in basic research and treatment

Method used

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  • Gold nanoparticle-liposome composite nanoparticles, pharmaceutical composition and applications thereof
  • Gold nanoparticle-liposome composite nanoparticles, pharmaceutical composition and applications thereof
  • Gold nanoparticle-liposome composite nanoparticles, pharmaceutical composition and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This example is used to illustrate the preparation method of the gold nano-liposome composite nanoparticles provided by the present invention.

[0035] 1. Preparation of Gold Nanoparticles

[0036] The process of preparing the gold nanoparticle dispersion by the seed growth method is as follows:

[0037] (1) Preparation of gold seeds

[0038] Get cetyltrimethylammonium bromide CTAB solution, add chloroauric acid (HAuCl 4 ) in aqueous solution, adding sodium borohydride (NaBH 4 ) solution, stirred for 3min to obtain the gold species for subsequent use;

[0039] Wherein, the amount of substance of CTAB: the amount of substance of chloroauric acid: the amount of substance of sodium borohydride=7500:24.8:6;

[0040] (2) Growth solution preparation

[0041] Take CTAB solution, add chloroauric acid (HAuCl 4 ), aqueous solutions of silver nitrate, aqueous sulfuric acid and ascorbic acid;

[0042] Wherein, the amount of substance of CTAB: the amount of substance of chlor...

Embodiment 2～10

[0058] This example is used to illustrate the preparation method of the gold nano-liposome composite nanoparticles provided by the present invention.

[0059] The preparation method described in Example 1 was used to prepare the gold nano-liposome composite nanoparticles.

[0060] Among them, the molar ratios of gold nanoparticles, plasmids, and membrane-penetrating peptides, and the molar ratios of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride, and cholesterol are as follows:

[0061]

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Abstract

The present invention provides gold nanoparticle-liposome composite nanoparticles, which are gold nanoparticles encapsulated with a liposome vesicle, wherein the surface of the liposome vesicle is modified with distearoyl phosphatidylethanolamine-polyethylene glycol, the surface of the gold nanoparticles is modified with plasmid and a penetrating peptide, and the liposome vesicle comprises dioleoyl phosphoethanolamine, 2,3-dioleyloxypropyltrimethyl ammonium chloride and cholesterol. The invention further provides a preparation method of the gold nanoparticle-liposome composite nanoparticles ,and a pharmaceutical composition comprising the gold nanoparticle-liposome composite nanoparticles. According to the present invention, the gold nanoparticle-liposome composite nanoparticles as the carrier have the high transfection effect, and the pharmaceutical composition has good treatment effects on various tumors.

Description

technical field [0001] The invention belongs to the technical field of medicine. Specifically, the present invention provides a gold nano-liposome composite nanoparticle and its pharmaceutical composition and application. Background technique [0002] At present, in the gene therapy of various tumors, the commonly used vectors mainly include viral vectors and non-viral vectors. Although viral vectors exhibit excellent gene transfection efficiencies, they can induce mutagenesis and other oncogenic effects. Furthermore, repeated use of viral vectors may induce immunodeficiency. Commonly used non-viral vectors include polymers, nanoparticles (magnetic nanoparticles, gold nanoparticles, carbon nanoparticles with different surface modifications) and commercial reagents (Lipofectamine2000, Lipofectamine3000), etc., have low transfection efficiency and cannot play a role in cancer. to a good gene therapy effect. At present, the use of gold nanoparticles is largely used for phot...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/24A61K47/02A61K47/46A61K48/00A61K38/46A61P35/00C12N15/85
CPCA61K9/1271A61K38/46A61K47/02A61K47/24A61K47/42A61K47/46C12N15/85C12N2800/107C12N2800/80C12N2810/10
Inventor 蒋兴宇王鹏郑文富张灵敏
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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