The invention relates to the technical field of pet food preparation, and particularly relates to a composition for removing dog tear stains, a product for removing dog tear stains and a preparation method thereof. The composition for removing dog tear stains is prepared from 1-2 parts of carrot powder, 0.5-1 part of fructus lycii, 0.1-0.5 part of semen cassiae, 0.1-0.2 part of lipase, 1-2 parts of tea polyphenol, 0.01 part of vitamin A, 0.03 part of vitamin E and 0.05 part of vitamin C. The product for removing dog tear stains is prepared from 2.79-5.79 parts of the composition for removing dog tear stains, 20-30 parts of fresh chicken, 20-30 parts of corn flour, 10-15 parts of chicken meal, 5-10 parts of chicken oil, 5-10 parts of fermented soybean meal and 5-10 parts of wheat flour. The preparation method comprises the steps that the fresh chicken is prepared into meat paste, then the corn flour, the chicken meal, the fermented soybean meal, the wheat flour, the carrot powder, the fructus lycii, the semen cassiae and the tea polyphenol are prepared into powder and mixed with the meat paste, puffing granulation and drying are conducted, the chicken oil, the lipase, the vitamin A, the vitamin E and the vitamin C are sprayed, and the product for removing dog tear stains is obtained. The product for removing dog tear stains has a very good tear stain removing effect.