Feed additive containing low-temperature glucose oxidase and application of feed additive

A glucose oxidase and feed additive technology, applied in the field of livestock and poultry breeding, can solve the problems of increased resistance to bacteria

Inactive Publication Date: 2019-06-11
DALIAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the deepening of research, the problems brought about by antibiotics have also increased. Incorrect use of antibiotics will lead to an increase in the resistance level of pathogenic bacteria. At present, the problem of drug-resistant bacteria has attracted widespread attention in the society.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation of Feed Antibacterial Additive Containing Low Temperature Glucose Oxidase

[0024] A feed antibacterial additive containing low-temperature glucose oxidase, composed of low-temperature glucose oxidase and silicon dioxide, the low-temperature glucose oxidase is produced from a glucose oxidase-producing Citrobacter sp. 8-Ⅲ The strain, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on January 24, 2019, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Zip Code: 100101, its The biological deposit number is CGMCC NO.17228.

[0025] One, the preparation of glucose oxidase:

[0026] Step 1. Inoculate the strains preserved in the laboratory in LB liquid medium at a ratio of 2%, and cultivate them at 20° C. and 160 rpm for 48 hours.

[0027] LB medium: 1% sodium chloride, 0.5% yeast extract, 1% peptone;

[0028]...

Embodiment 2

[0037] Antibacterial properties of feed additives

[0038] The feed additive is formed by compounding the low-temperature glucose oxidase and an appropriate carrier, and the carrier is silicon dioxide. This can avoid inactivation or denaturation of the low-temperature glucose oxidase due to direct exposure to the environment or contact with other ingredients in the feed during use.

[0039] The feed additive can be directly added to the animal feed to feed the animals, and the feed additive can be fed to the animals after being evenly mixed with the basic ration of the animal feed, all of which can be ingested by the animals to achieve the antibacterial effect , when in use, the added amount of the feed additive accounts for 0.05% of the total mass of the animal feed as the criterion.

[0040] The indicated feed additives (low temperature glucose oxidase mixed with silica 4:1) were added at 0.05% to the chicken feed. The unsupplemented one was used as the control group, and ...

Embodiment 3

[0045] Effect of Feed Additives on Feed Mildew Rate

[0046] The indicated feed additives (low temperature glucose oxidase mixed with silica 4:1) were added at 0.05% in puppy feed. The unsupplemented feed was used as the control group, and the experimental group was divided into three groups: A, B, and C. Each group had 25 servings of 0.5 kg feed, and placed it at 23°C for 7 days to observe the mildew. The recorded data are shown in Table 2.

[0047] Table 2 Record Form of Mildew Rate of Puppies Feed

[0048] project

control group

Group A

Group B

Group C

Mildew rate on the first day (%)

0

0

0

0

Mildew rate on the second day (%)

0

0

0

0

Mildew rate on the third day (%)

8

0

0

0

Mildew rate on the fourth day (%)

16

0

4

0

Mildew rate on the fifth day (%)

28

8

12

12

Mildew rate on the sixth day (%)

40

12

16

12

Mil...

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PUM

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Abstract

The invention relates to the technical field of livestock and poultry breeding, in particular to a feed antibacterial additive containing low-temperature glucose oxidase and application of the feed antibacterial additive. The feed additive comprises glucose oxidase and silicon dioxide, and the glucose oxidase is produced from a citrobacter sp. 8-III strain which produces glucose oxidase, has a preservation number of CGMCC NO.17228 adn and has low-temperature enzyme characteristics. When the antibacterial feed additive is used, the antibacterial feed additive is directly added into feed, and the mildew rate of the feed can be reduced when the feed is stored at 23 DEG C. The antibacterial feed additive provided by the invention comprises glucose oxidase and has the advantages of high safetyand no drug resistance; and the feed additive is capable of regulating the balance of intestinal flora, generating an environment beneficial to the growth of beneficial bacteria, promoting the growthof animals, improving the feed conversion efficiency and reducing the incidence rate of diseases by inhibiting the reproduction of harmful microorganisms in animal intestinal tracts, can effectively replace antibiotics, conforms to the development direction of healthy breeding and has a good application prospect in the field of feeds.

Description

technical field [0001] The invention relates to the technical field of livestock and poultry breeding, in particular to a feed antibacterial additive containing low-temperature glucose oxidase and its application. Background technique [0002] Antibiotics have been used in aquaculture for nearly a hundred years, and their functions of preventing diseases and promoting growth have been widely used. More than 90% of the world's animal feed contains antibiotics and their analogues, most of which are used to promote growth and improve feed conversion. For the modern aquaculture industry, antibiotic rope undoubtedly plays an important role, reflecting significant economic benefits. However, with the deepening of research, the problems brought about by antibiotics have also increased. Incorrect use of antibiotics will lead to an increase in the resistance level of pathogenic bacteria. At present, the problem of drug-resistant bacteria has attracted widespread attention in the soc...

Claims

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Application Information

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IPC IPC(8): A23K20/189A23K20/20A23K50/75A23K50/40C12N9/04C12R1/01
Inventor 张庆芳胡善松迟乃玉刘春莹李美玉
Owner DALIAN UNIVERSITY
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