Feed additive containing low-temperature glucose oxidase and application of feed additive
A glucose oxidase and feed additive technology, applied in the field of livestock and poultry breeding, can solve the problems of increased resistance to bacteria
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Embodiment 1
[0023] Preparation of Feed Antibacterial Additive Containing Low Temperature Glucose Oxidase
[0024] A feed antibacterial additive containing low-temperature glucose oxidase, composed of low-temperature glucose oxidase and silicon dioxide, the low-temperature glucose oxidase is produced from a glucose oxidase-producing Citrobacter sp. 8-Ⅲ The strain, which has been preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on January 24, 2019, address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Zip Code: 100101, its The biological deposit number is CGMCC NO.17228.
[0025] One, the preparation of glucose oxidase:
[0026] Step 1. Inoculate the strains preserved in the laboratory in LB liquid medium at a ratio of 2%, and cultivate them at 20° C. and 160 rpm for 48 hours.
[0027] LB medium: 1% sodium chloride, 0.5% yeast extract, 1% peptone;
[0028]...
Embodiment 2
[0037] Antibacterial properties of feed additives
[0038] The feed additive is formed by compounding the low-temperature glucose oxidase and an appropriate carrier, and the carrier is silicon dioxide. This can avoid inactivation or denaturation of the low-temperature glucose oxidase due to direct exposure to the environment or contact with other ingredients in the feed during use.
[0039] The feed additive can be directly added to the animal feed to feed the animals, and the feed additive can be fed to the animals after being evenly mixed with the basic ration of the animal feed, all of which can be ingested by the animals to achieve the antibacterial effect , when in use, the added amount of the feed additive accounts for 0.05% of the total mass of the animal feed as the criterion.
[0040] The indicated feed additives (low temperature glucose oxidase mixed with silica 4:1) were added at 0.05% to the chicken feed. The unsupplemented one was used as the control group, and ...
Embodiment 3
[0045] Effect of Feed Additives on Feed Mildew Rate
[0046] The indicated feed additives (low temperature glucose oxidase mixed with silica 4:1) were added at 0.05% in puppy feed. The unsupplemented feed was used as the control group, and the experimental group was divided into three groups: A, B, and C. Each group had 25 servings of 0.5 kg feed, and placed it at 23°C for 7 days to observe the mildew. The recorded data are shown in Table 2.
[0047] Table 2 Record Form of Mildew Rate of Puppies Feed
[0048] project
control group
Group A
Group B
Group C
Mildew rate on the first day (%)
0
0
0
0
Mildew rate on the second day (%)
0
0
0
0
Mildew rate on the third day (%)
8
0
0
0
Mildew rate on the fourth day (%)
16
0
4
0
Mildew rate on the fifth day (%)
28
8
12
12
Mildew rate on the sixth day (%)
40
12
16
12
Mil...
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