31results about How to "Avoid repeated freezing and thawing" patented technology

The invention discloses a DNA (deoxyribonucleic acid) preserving solvent and a DNA preserving method. The DNA preserving solvent comprises glycerol, TE buffer solution and 1-carboxyl-N, N, N-trimethyl-B lactone, wherein the final volume of the glycerol is 25-80%, the final concentration of the TE buffer solution is 1*TE-5*TE, and the final concentration of the 1-carboxyl-N, N, N-trimethyl-B lactone is 1-6mol / L. The DNA preserving method comprises the following steps of: (1) extracting DNA; and (2) dissolving the DNA in the DNA preserving solvent to be preserved. The DNA preserving solvent disclosed by the invention has the advantages of simple preparation, low cost, good preserving effect and long shelf life and is prevented from freezing and thawing repeatedly. The invention provides initial DNA records for genomics research, identity authentication, gene diagnosis, gene therapy, organ transplantation and the like in the future.

The invention relates to a freeze-drying protective agent of an SNP detection reagent, and a protection method. The freeze-drying protective agent comprises trehalose, glucan and gelatin. The protective agent disclosed by the invention realizes a purpose of constant-temperature transportation and constant-temperature preservation of the SNP detection reagent, and transportation cost is dramatically lowered. The freeze-drying protective agent is simple to operate, and pollution brought by repeated freeze thawing of the reagent and repeated utilization of the SNP detection reagent can be avoided.

The invention discloses an NAT2 (N-acetyltransferase-2) gene polymorphism fluorescence PCR (Polymerase Chain Reaction) melting curve detection kit, which comprises a first probe, a second probe, a third probe, a fourth probe, a positive primer and a reverse primer, wherein different fluorescence groups are respectively marked at 5' ends of the first probe, the second probe, the third probe and the fourth probe; and corresponding quenching groups are marked at the 3' ends of the first probe, the second probe, the third probe and the fourth probe. The detection of four mononucleotide polymorphisms on four sites can be completed in a single-tube PCR system; the sample genotype can be known through once fluorescence PCR melting curve analysis after the PCR amplification is completed; the whole operation can be completed in 3 hours; and the time consumption is low.

The invention relates to an automatic liquid nitrogen storage tank. The automatic liquid nitrogen storage tank comprises a liquid nitrogen tank, a freezing storage mechanism and a lifting mechanism; atank port is formed in the top of the liquid nitrogen tank; the freezing storage mechanism comprises a plurality of freezing storage frames; the multiple freezing storage frames are evenly distributed in the liquid nitrogen tank with the center of the liquid nitrogen tank as a center of a circle, a plurality of placing plates are sequentially arranged on each freezing storage frame from top to bottom, cross-shaped placing grooves are correspondingly formed in the tops of the placing plates, a plurality of freeing storage boxes are correspondingly arranged in each cross-shaped placing groove in a cross-shaped distribution mode, and each freezing storage frame is connected with the liquid nitrogen tank through a first rotating mechanism; the lifting mechanism comprises a supporting frame; and a lifting plate is arranged on the supporting frame, the lifting plate is connected with the supporting frame through the lifting mechanism, and the bottom of the lifting plate is connected with the top of an installation plate through a second rotating mechanism. The automatic liquid nitrogen storage tank has the beneficial effects that the freezing storage boxes required by the liquid nitrogen tank can be accurately taken out, the degree of automation is high, the difficulty of manual operation is lowered, repeated freezing and thawing of samples is avoided, and the storage quality of thesamples is improved.

The present invention relates to external liquid and solid kreotoxin (argireline) composite nanometer latex for skin and a preparation method thereof. The weight proportion of each raw material of liquid nanometer latex is 10g of isopropyl myristate, 2g of vitamin E, 0.8g of vitelline lecithin, 0.2g of ginseng saponin, 0.01g of rhodiola element, 0.005g of the argireline, 0.001g of superoxide dismutase, 0.5g of reduction type glutathione, 5g of fetal calf serum, 0.05g of hyaluronic acid, 0.05g of ceramide, 40g of distilled water, 36g of polyethylene glyceride glycol caprylic decanoate and 12g of polyglycerol fatty acid ester; the weight proportion of each raw material of solid nanometer latex is 10g of the isopropyl myristate, 2g of the vitamin E, 0.8g of the vitelline lecithin, 0.2g of the ginseng saponin, 0.01g of the rhodiola element, 0.005g of the argireline, 0.001g of the superoxide dismutase, 0.5g of the reduction type glutathione, 5g of the fetal calf serum, 0.05g of the hyaluronic acid, 0.05g of the ceramide, 40g of the distilled water, 36g of the polyethylene glyceride glycol caprylic decanoate, 12g of the polyglycerol fatty acid ester, 22g of mannitol and 3g of glycin.

The invention provides a method for developing fruit cakes by utilizing blueberry juice residual residues as a raw material. The method comprises the following steps: directly performing vacuum freezing chopping treatment on frozen blueberry residues without melting and pulping, accelerating the dissolution of active ingredients in a manner of combining ultrasonic emulsification treatment with proper heat treatment, wherein the manner replaces a traditional boiling process, performing high-pressure pasteurization, and finally cooling and molding. Part of macromolecular hydrophilic colloid is added before sterilization, and the gel strength and the shape maintenance are improved. The method is simple to operate, low in heat treatment strength and short in time, and repeated freeze thawing and high-strength heat treatment of a product are avoided. Therefore, the method has the advantages that the microbial pollution probability is low, the retention rate of the effective active ingredients is high, the sensory quality is excellent, the shape maintenance at the temperature of 37-42 DEG C is good and the like.

The invention provides a biological sample bank tissue cryopreservation method and a biological sample bank tissue cryopreservation tube. The freezing method comprises: placing the biological sample bank tissue on the surface of a carrier sheet; The slices are placed in the tissue cryopreservation tube of the biobank, and the tissue cryopreservation tube of the biobank is sealed and stored at a low temperature; the cryopreservation tube includes a tube body, a cap and at least one for holding the separated biobank tissue. The carrier sheet can be detachably placed in the tube body; this cryopreservation method can solve the problem that the tissue of the biological sample bank is not easy to be identified or taken out because it is pasted on the inner wall of the cryopreservation tube after cryopreservation. It can also avoid repeated freezing and thawing of biological sample bank tissue when making frozen sections, thereby benefiting the protection of biological sample bank tissue quality (for example, biological macromolecules such as RNA, protein, antigen and antibody contained).

The invention relates to a bone polypeptide product, and in particular relates to a pharmaceutical composition containing animal bone polypeptide. The pharmaceutical composition comprises the following components in parts by weight: 100 parts of polypeptide injection stock solution extracted from pig bones, cow bones and deer bones, 0.1-5 parts of chloretone and 0.5-5 parts of sodium dihydrogen phosphate. The pharmaceutical composition containing animal bone polypeptide is good in stability, extremely low in high polymer content, high in safety and extremely suitable for clinic application.

The invention provides a cryopreserving method for a biological tissue sample and a biological tissue cryopreserving container. The cryopreserving method comprises the steps that the biological tissue is arranged on the surface of a bearing piece, the bearing piece to which the biological tissue is attached is placed in the biological tissue cryopreserving container in the long axis direction of the biological tissue cryopreserving container, and the biological tissue cryopreserving container is sealed and frozen at the low temperature; and the cryopreserving container comprises a container body, a sealing cover and at least on bearing piece used for bearing the separated biological tissue and separably arranged in the container body. According to the cryopreserving method, the problems that due to the fact that the biological tissue is prone to being cryopreserving on the inner wall of the biological tissue cryopreserving container, the biological tissue is not prone to being recognized or being taken out or being damaged after being taken out can be solved, the situation of multigelation when the biological tissue is made into cryopreserved sections can be avoided, and the protection of the sample quality (such as the contained RNA, proteins, antigens, antibodies and other biomacromolecules) of the biological tissue is facilitated.

The invention belongs to the technical field of vaccines, and discloses a preparation method and application of a polypeptide vaccine. The preparation method comprises the following steps: respectively cleaning a packaging material penicillin bottle body, a bottle cap and a bottle plug, and sterilizing; preparing a mixed solution of normal saline containing 0.5% DMSO and DMSO as a solvent, and oscillating and uniformly mixing the solvent for later use; adding the solvent into a polypeptide freeze-dried powder, blowing and beating for 10 times, and uniformly mixing; sterilizing and filtering anobtained polypeptide-containing solution, and sub-packaging a filtered solution into the cleaned penicillin bottles. According to the method, microorganisms and residual endotoxin in the penicillin bottles are removed through dry heat sterilization, and bottle caps and bottle plugs are sterilized through high-temperature and high-pressure moist heat sterilization. According to the invention, a solvent which has little damage to polypeptide, can prevent side chain oxidation and can dissolve polypeptide is used. The polypeptide with the concentration prepared by the invention is stored at -80 DEG C, is stable in property and can be stored for a long time. The process provided by the invention is suitable for preparing personalized customized vaccines and meets the sterile and safety requirements specified in pharmacopeia.

The invention discloses an automatic preservation / extraction control device for frozen cells. The automatic preservation / extraction control device comprises a heat insulation system, an automatic preservation / extraction control system, a bar code identification system, a temperature detection system, a central control system and a liquid nitrogen capacity detection system, wherein the heat insulation system comprises two layers of hollow heat insulated boxes, an intermediate sandwich layer is filled with a heat insulating material, and liquid nitrogen adsorbents are mounted inside the heat insulated boxes; the automatic preservation / extraction control system comprises a rotary sealing door and a cell cryopreservation shelf, cell freezing boxes are placed on each layer of the cell cryopreservation shelf, and guide rails which are used for enabling the cell cryopreservation shelf to move up and down are arranged at the two sides of the cell cryopreservation shelf; the automatic preservation / extraction control system further comprises a servomotor driving screw rod and hooks, the hooks are connected with the cell cryopreservation shelf, and the cell cryopreservation shelf is driven to move up and down under the driving of the servomotor driving screw rod; and the automatic preservation / extraction control system comprises an electric push rod, an ejector pin is arranged at the top end of the electric push rod, and the cell freezing boxes are pushed to an extraction window under the driving of the electric push rod. According to the automatic preservation / extraction control device for the frozen cells, the quality of the frozen cells can be guaranteed, and the efficiency of preservation / extraction of the frozen cells is increased.

The invention discloses a novel coronavirus (2019‑nCoV) double fluorescence freeze-dried microchip, a kit and a method, the kit including a freeze-dried microchip, a tube of mineral oil, a tube of positive control, a tube of negative control, A tube of diluent and a tube of nuclease-free water, the lyophilized microchip is coated with primers, probes, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP, Mg 2+ . The freeze-drying conditions are as follows: in the pre-freezing stage, the temperature of the clapboard drops to -55°C, and the holding time is 1h during pre-freezing, and then the equipment is vacuumed to keep freeze-drying for 1h; in the analytical drying stage, the temperature of the clapboard is raised to -25°C Keep it at ℃ for 1h, then raise the temperature of the separator to 37℃ and keep it for 2h, and finally lower the temperature of the separator to 25℃ and keep it for 1h to obtain a freeze-dried microchip, just add diluent and sample nucleic acid when using it. The invention detects ORF 1a / b gene and N gene simultaneously, and the detection method of the detection kit has high accuracy, specificity and sensitivity, and the detection time is short.

The invention discloses an economic and practical method for extracting and separating bone tissue protein and belongs to the technical field of molecular biology. The method concretely comprises the following steps: placing a weighted centrifugal tube onto ice and pre-cooling; taking a phosphatase inhibitor, a protease inhibitor and phenylmethylsulfonyl fluoride, adding the three into a splitting buffer solution, and then placing the mixture onto the ice and uniformly stirring; weighting 0.3-0.5g of femoral shaft; putting the femoral shaft into a grinding kettle filled with liquid nitrogen and grinding the femoral shaft into powder; transferring the femoral shaft to a spare centrifugal tube and weighting, thereby obtaining the weight of the femoral shaft; multiplying the mass by 1 / 3, and then adding the lysate into the centrifugal tube in concentration of 0.2-0.4g / mL; shaking the mixed solution under a low temperature and then standing; placing the turbid liquid under the conditions of 4 DEG C and 3000-5000rpm and centrifuging; and absorbing the supernate to a new centrifugal tube and then split-charging and storing. According to the invention, the extraction and separation process is simple and the separated protein purity is high, so that the extraction and separation process is suitable for popularization and application in the field of biology.

The invention relates to a concentrated anti-freeze alga polypeptide liquid and a preparation method thereof, and belongs to the technical field of food processing. The concentrated anti-freeze alga polypeptide liquid is prepared from, by weight, 3-10 parts of concentrated anti-freeze laver liquid, 1-10 parts of marine polysaccharid, 0.2-0.5 part of phosphate and 30-100 parts of deionized water. New raw materials with a low cost are provided for anti-freeze polypeptide, and the extraction rate of anti-freeze polypeptide is increased to achieve industrialization and expand the application field.