31results about How to "Avoid repeated freezing and thawing" patented technology

The present invention relates to external liquid and solid kreotoxin (argireline) composite nanometer latex for skin and a preparation method thereof. The weight proportion of each raw material of liquid nanometer latex is 10g of isopropyl myristate, 2g of vitamin E, 0.8g of vitelline lecithin, 0.2g of ginseng saponin, 0.01g of rhodiola element, 0.005g of the argireline, 0.001g of superoxide dismutase, 0.5g of reduction type glutathione, 5g of fetal calf serum, 0.05g of hyaluronic acid, 0.05g of ceramide, 40g of distilled water, 36g of polyethylene glyceride glycol caprylic decanoate and 12g of polyglycerol fatty acid ester; the weight proportion of each raw material of solid nanometer latex is 10g of the isopropyl myristate, 2g of the vitamin E, 0.8g of the vitelline lecithin, 0.2g of the ginseng saponin, 0.01g of the rhodiola element, 0.005g of the argireline, 0.001g of the superoxide dismutase, 0.5g of the reduction type glutathione, 5g of the fetal calf serum, 0.05g of the hyaluronic acid, 0.05g of the ceramide, 40g of the distilled water, 36g of the polyethylene glyceride glycol caprylic decanoate, 12g of the polyglycerol fatty acid ester, 22g of mannitol and 3g of glycin.

The invention provides a method for developing fruit cakes by utilizing blueberry juice residual residues as a raw material. The method comprises the following steps: directly performing vacuum freezing chopping treatment on frozen blueberry residues without melting and pulping, accelerating the dissolution of active ingredients in a manner of combining ultrasonic emulsification treatment with proper heat treatment, wherein the manner replaces a traditional boiling process, performing high-pressure pasteurization, and finally cooling and molding. Part of macromolecular hydrophilic colloid is added before sterilization, and the gel strength and the shape maintenance are improved. The method is simple to operate, low in heat treatment strength and short in time, and repeated freeze thawing and high-strength heat treatment of a product are avoided. Therefore, the method has the advantages that the microbial pollution probability is low, the retention rate of the effective active ingredients is high, the sensory quality is excellent, the shape maintenance at the temperature of 37-42 DEG C is good and the like.

The invention provides a biological sample bank tissue cryopreservation method and a biological sample bank tissue cryopreservation tube. The freezing method comprises: placing the biological sample bank tissue on the surface of a carrier sheet; The slices are placed in the tissue cryopreservation tube of the biobank, and the tissue cryopreservation tube of the biobank is sealed and stored at a low temperature; the cryopreservation tube includes a tube body, a cap and at least one for holding the separated biobank tissue. The carrier sheet can be detachably placed in the tube body; this cryopreservation method can solve the problem that the tissue of the biological sample bank is not easy to be identified or taken out because it is pasted on the inner wall of the cryopreservation tube after cryopreservation. It can also avoid repeated freezing and thawing of biological sample bank tissue when making frozen sections, thereby benefiting the protection of biological sample bank tissue quality (for example, biological macromolecules such as RNA, protein, antigen and antibody contained).

The invention relates to a bone polypeptide product, and in particular relates to a pharmaceutical composition containing animal bone polypeptide. The pharmaceutical composition comprises the following components in parts by weight: 100 parts of polypeptide injection stock solution extracted from pig bones, cow bones and deer bones, 0.1-5 parts of chloretone and 0.5-5 parts of sodium dihydrogen phosphate. The pharmaceutical composition containing animal bone polypeptide is good in stability, extremely low in high polymer content, high in safety and extremely suitable for clinic application.

The invention provides a cryopreserving method for a biological tissue sample and a biological tissue cryopreserving container. The cryopreserving method comprises the steps that the biological tissue is arranged on the surface of a bearing piece, the bearing piece to which the biological tissue is attached is placed in the biological tissue cryopreserving container in the long axis direction of the biological tissue cryopreserving container, and the biological tissue cryopreserving container is sealed and frozen at the low temperature; and the cryopreserving container comprises a container body, a sealing cover and at least on bearing piece used for bearing the separated biological tissue and separably arranged in the container body. According to the cryopreserving method, the problems that due to the fact that the biological tissue is prone to being cryopreserving on the inner wall of the biological tissue cryopreserving container, the biological tissue is not prone to being recognized or being taken out or being damaged after being taken out can be solved, the situation of multigelation when the biological tissue is made into cryopreserved sections can be avoided, and the protection of the sample quality (such as the contained RNA, proteins, antigens, antibodies and other biomacromolecules) of the biological tissue is facilitated.

The invention discloses an automatic preservation / extraction control device for frozen cells. The automatic preservation / extraction control device comprises a heat insulation system, an automatic preservation / extraction control system, a bar code identification system, a temperature detection system, a central control system and a liquid nitrogen capacity detection system, wherein the heat insulation system comprises two layers of hollow heat insulated boxes, an intermediate sandwich layer is filled with a heat insulating material, and liquid nitrogen adsorbents are mounted inside the heat insulated boxes; the automatic preservation / extraction control system comprises a rotary sealing door and a cell cryopreservation shelf, cell freezing boxes are placed on each layer of the cell cryopreservation shelf, and guide rails which are used for enabling the cell cryopreservation shelf to move up and down are arranged at the two sides of the cell cryopreservation shelf; the automatic preservation / extraction control system further comprises a servomotor driving screw rod and hooks, the hooks are connected with the cell cryopreservation shelf, and the cell cryopreservation shelf is driven to move up and down under the driving of the servomotor driving screw rod; and the automatic preservation / extraction control system comprises an electric push rod, an ejector pin is arranged at the top end of the electric push rod, and the cell freezing boxes are pushed to an extraction window under the driving of the electric push rod. According to the automatic preservation / extraction control device for the frozen cells, the quality of the frozen cells can be guaranteed, and the efficiency of preservation / extraction of the frozen cells is increased.

The invention discloses a novel coronavirus (2019‑nCoV) double fluorescence freeze-dried microchip, a kit and a method, the kit including a freeze-dried microchip, a tube of mineral oil, a tube of positive control, a tube of negative control, A tube of diluent and a tube of nuclease-free water, the lyophilized microchip is coated with primers, probes, Taq enzyme, reverse transcriptase, trehalose, Tris-Cl, dNTP, Mg 2+ . The freeze-drying conditions are as follows: in the pre-freezing stage, the temperature of the clapboard drops to -55°C, and the holding time is 1h during pre-freezing, and then the equipment is vacuumed to keep freeze-drying for 1h; in the analytical drying stage, the temperature of the clapboard is raised to -25°C Keep it at ℃ for 1h, then raise the temperature of the separator to 37℃ and keep it for 2h, and finally lower the temperature of the separator to 25℃ and keep it for 1h to obtain a freeze-dried microchip, just add diluent and sample nucleic acid when using it. The invention detects ORF 1a / b gene and N gene simultaneously, and the detection method of the detection kit has high accuracy, specificity and sensitivity, and the detection time is short.

The invention relates to a concentrated anti-freeze alga polypeptide liquid and a preparation method thereof, and belongs to the technical field of food processing. The concentrated anti-freeze alga polypeptide liquid is prepared from, by weight, 3-10 parts of concentrated anti-freeze laver liquid, 1-10 parts of marine polysaccharid, 0.2-0.5 part of phosphate and 30-100 parts of deionized water. New raw materials with a low cost are provided for anti-freeze polypeptide, and the extraction rate of anti-freeze polypeptide is increased to achieve industrialization and expand the application field.