64 results about "Dna storage" patented technology

The invention belongs to the field of molecular biology, specifically belongs to the technical field of DNA storage, more specifically an artificially synthesized DNA storage medium with coding information, a storage reading method for the information, and applications. Discarding DNA storage technology depended on a mature binary code or a ternary code in the prior art, the invention creatively discloses a DNA storage mode capable of coding directly. Through using storage method capable of coding directly, especially tetragenous basic group coding, the obtained DNA chain storing digit information is short in entire sequence and average content of guanine and cytosine in the sequence is relatively balanced. The method of the invention is in favour of synthesizing, convenient for decoding, and could reduce error rate. The method of the invention can be used for covering a plurality of character types such as English, figure, Chinese and punctuation marks with wide application range.

The present invention relates to an encoding method and an decoding method for performing information storage by means of DNA. Different from a conventional computer magnetic medium, an information write-in mode of DNA storage is that after information is encoded, an oligonucleotide chain with a certain length is synthesized by utilizing an oligonucleotide chain synthesis technology and the synthesized oligonucleotide chain is stored in a powder form; and a reading technology of DNA storage is that the oligonucleotide chain is sequenced by utilizing a high-throughput sequencing technology and after being spliced, sequenced fragments are transcoded, so that an initial computer multimedia file can be restored. Due to the characteristics of the DNA oligonucleotide chain, in the design of an encoding mode, a random error possibly existing in the DNA synthesis and sequencing process can be taken into full consideration, and error authentication and multiple cover segment are performed on the DNA fragments. The encoding method for a DNA storage technology, which is constructed by the present invention, is simple, convenient and easy to operate and can be applied to transform the computer multimedia files in various formats into DNA sequences so as to perform information storage.

The invention discloses a DNA storage, encryption and encoding method. The method comprises the following steps of: inputting original data to perform binary conversion, performing Huffman encoding compression, and converting the input original data into a DNA sequence according to a DNA storage quaternary model; selecting a file as a secret key to carry out binary conversion, and then convertingthe file into a DNA sequence; encrypting a storage sequence by adopting a secret key; dividing the storage sequence into a plurality of columns with an equal length, and adding an address code to thehead of each column; adopting rS coding to correct each column, wherein the decoding process is a reverse process of coding, splicing is carried out according to address codes, and the address codes and error correction codes are deleted; decrypting the storage sequence according to the encryption method and the key sequence; and carrying out Huffman decoding to obtain the input file again. According to the method, encryption is carried out on the basis of the DNA quaternary model, the storage confidentiality is improved, extremely high coding potential is obtained, GC percentage and average content balance of basic groups G and C can be better controlled, synthesis is facilitated, and the error rate is low.

A method of tracking a provenance of a fur or a leather skin includes providing at least one of a live animal, a fur pelt, and a leather skin, removing a DNA sample from the at least one of the live animal, the fur pelt, and the leather skin, and storing the DNA sample. The DNA sample and DNA information is stored in a DNA repository that may be securely accessed by authorized individuals through a networked device, such as a computer, smart phone, or tablet. The method further includes assigning a globally unique identifier number to the at least one of the live animal, the fur pelt, and the leather skin and storing the identifier number. The method further includes selling the at least one of the live animal, the fur pelt, and the leather skin and storing sale information and associating the sale information with at least one of the identifier number and the DNA sample.

The present invention discloses a method and a system for monitoring and tracing transmission of multimedia resources, wherein, said method comprises: capturing data packets in a network link, reassembling the captured data packets to complete the restoration of data streams, and storing the restored data streams into a data stream database; and extracting data fragments from a multimedia file as file DNA fragments to construct a file DNA, and storing the file DNA into a file DNA database; and invoking the file DNA from the file DNA database, and identifying the file DNA fragments of the file DNA in the data streams to get identification results.

The invention relates to a DNA storage method based on oligonucleotide sequence code storage. The DNA storage method based on oligonucleotide sequence code storage performs oligonucleotide sequence coding on different binary string segments in a variety of different base coding formats, and each set of binary coded file fragments can always find an oligonucleotide sequence code that satisfies therequirements for synthesis and sequencing, thus greatly simplifying coding of binary string segments of oligonucleotides and the conversion of coded oligonucleotides into binary string segments for operation, realizing efficient coding and decoding operations, and maximally utilizing the data storage capacity of DNA molecules while simplifying codes of binary string segments and the operation of restoring the coded oligonucleotide sequence to binary string segments. In addition, in coded binary character segments, the maximum theoretical value of each base coded binary is 2 bytes, thus reducing the number or length of oligonucleotide sequences, reducing the cost of oligonucleotide synthesis and sequencing, and having the largest information storage amount.

The invention discloses a DNA storage coding optimization method based on a K-means clustering multivariate universe algorithm, and the method specifically comprises the steps: constructing an optimalDNA coding sequence meeting a combination constraint condition, firstly constructing a certain number of DNA sequences as an initial population, and carrying out the evaluation and sorting of the fitness of the population; secondly, optimizing the obtained DNA coding sequence by using a k-means clustering algorithm and wormhole intersection to obtain a DNA coding sequence with higher fitness; then, judging whether to add an alternative solution set or not according to constraints through constraint comparison; and finally, outputting an optimal DNA coding sequence. According to the method, DNA coding sequences with relatively good quantity can be searched.

The invention discloses a DNA storage coding method for optimizing Chinese storage, which comprises the following steps: 1) inputting a Chinese text, and recoding first-level Chinese characters or first-level and second-level Chinese characters according to the type of contained characters and the GB2312-80 standard; 2) counting the occurrence frequency of the segmented words in the text, multiplying the occurrence frequency by the length of the segmented words, sorting the products, and encoding the segmented words ranked in the front column,3) converting all characters into a binary sequence, and carrying out Huffman coding compression, 4) converting into a DNA sequence, and adding an address code and an RS error correction code, 5) the decoding process being an encoding reverse process,firstly carrying outerror correction , then carrying out the sequence splicing, and converting the DNA sequence into a binary sequence. According to the method, the redundancy of the Chinese text isreduced, the DNA storage coding compression effect is improved, and extremely high Chinese coding potential is obtained.

The present invention relates to a biological sample storage liquid, particularly to a microorganism sample DNA storage solution, wherein a 0.5-2 mM / L EDTA, saturated sodium chloride (NaCl), methanol with a mass fraction of 2-5%, glycerol with a mass fraction of 5-15%, fatty alcohol polyoxyethylene ether with a mass fraction of 5-10%, sodium dodecylsulfate (SDS) with a mass fraction of 1-5% and mixed mineral oil are dissolved in pure water, and the pH value of the obtained solution is adjusted to 6.0-9.0. According to the present invention, the microorganism sample DNA storage solution is suitable for the room temperature storage of microorganism samples, has characteristics of simple operation and good applicability, and can be used in large-scale sample detection.

The invention discloses a DNA information storage method based on Raptor codes and quaternion RS codes. The method comprises a DNA information storage process based on Raptor codes. The DNA information storage process comprises the following steps: 1, converting an information file needing to be stored into a binary code stream; 2, encoding the binary code stream into a base sequence containing A,T, C and G by using Raptor codes; and 3, adding a quaternary RS error correction mechanism to the base sequences to ensure the correctness of information storage, then adding primer segments requiredfor synthesizing DNA to the two ends of the base sequences, and synthesizing the base sequences into DNA chains by using a biotechnology to complete information storage; compared with the prior art,the DNA storage technology realized by the invention has the beneficial effects of good ductility, high encoding and decoding efficiency, low complexity and the like.

An integral digital memory storage device having a standard form factor to be received by and communicating with a computing device and having memory capability for storage of digital data. An integral multiwell DNA sample tray is carried in a body of the memory storage device for protection and exposed by manipulation of the case for receiving DNA samples.

A method for encoding and storing text information using DNA as a storage medium, a decoding method therefor and an application thereof. The method for using DNA to store text information comprises: encoding characters into computer binary digits by means of encoding, and converting the binary digits into DNA sequences by means of transcoding; and artificially synthesizing the DNA sequences encoded with character information, positioning the characters by means of a designed ligation adapter, and assembling the DNA sequences encoded with the character information according to a pre-set order. The method for using DNA to store text information has the advantages of a small storage volume, a large storage capacity, a strong stability and low maintenance costs.

The invention discloses a DNA storage encoding and decoding method based on a hybrid model. The method comprises the following steps: inputting original data to perform binary conversion, and performing Huffman encoding compression; dividing the file into a plurality of columns, and adding an address code to the column head; storing the DNA in a quaternion and binary model for hybrid coding, and modifying an initial model code; adding an error correction code to the model code by adopting RS coding, and then performing RS coding error correction on the DNA sequence; repeating the above steps until all sequences are coded and corrected; sorting all the sequences according to file codes and coding numbers, and adding four columns of error correction sequences to every 123 columns by utilizing RS codes; according to the method, a traditional DNA storage quaternary model and a binary model are subjected to hybrid encoding, and the encoding potential reaches 1.75; compared with a quaternarymodel, the GC percentage can be better controlled, and compared with a binary model, the storage capacity is greatly improved.

The invention discloses a DNA storage method and system and a storage medium, and the method comprises the steps of carrying out the sequencing of synthetic DNA, and obtaining a plurality of sequencing sequences; determining that no corresponding sequencing base sequence exists in the coding table, and obtaining the base sequence with the smallest Hamming distance from the sequencing base sequencein the coding table to replace the sequencing base sequence; decoding the replaced sequencing sequence according to the coding table to obtain a decoded text, wherein the Hamming distance between anytwo base sequences in the character coding table is greater than a first threshold value. According to the embodiment of the invention, error correction is carried out on the sequencing base sequenceobtained by sequencing by utilizing the Hamming distance with the base sequence, and the sequencing base sequence after error correction is decoded to obtain a decoded text. Due to the fact that theHamming distance between any two base sequences in the coding table is larger than the first threshold value, the coding table has certain error correction capacity, and compared with existing redundant code error correction, the DNA storage efficiency is greatly improved. The invention can be widely applied to the field of molecular biology.

The invention discloses a preservation method for defunctive human DNA. The method comprises the following steps: (1), sampling and testing oral mucosa epithelial cells; (2), extracting DNA; (3), detecting the quality of the DNA; and (4), storing the DNA. The preservation method for the defunctive human DNA is jointly researched by famous geneticists at home and abroad, provides individualized DNA storage service and stores all lifetime information for a defunctive person, establishes a family gene pedigree for family numbers, records important family gene history for the gene comparison and the gene therapy of descendants in future and provides a material base for the descendants to thoroughly get rid of diseases with inherited tendency, such as malignant tumors, and the like.

The invention is a microflow control chip DNA molecular memory, comprising memory medium, reaction medium and microflow control chip, where the memory medium comprises known-sequence containing short-chain DNA memory cell molecules, initial operating DNA blank molecules and DNA memory molecules standing for superposed results by biochemical reaction; the microflow control chip is provided at least with memory cell region, endonuclease reaction region, enzyme-link reaction region, and result output region, where the endonuclease reaction region, enzyme-link reaction region, and result output region are interconnected through microchannel and the memory cell region is connected with the enzyme-link reaction region through microchannel. The invention adopts the microflow control chip technique to replace the widely used test tube or surface operation in the current DNA calculating course for the first time, and provides a practical possible platform for constructing a proper DNA computer by the character that the microflow control chip operates accurately and controllably and can be integrated in high flux and large scale.

The invention discloses a DNA storage hierarchical representation and interleaved coding method capable of containing artificial bases. The invention belongs to the field of DNA storage reliable coding, binary data is scrambled, error correction codes are grouped and then divided into a plurality of sub-blocks, different coding sub-blocks form oligonucleotide molecules according to a layering mode, and when the molecules are lost or basic groups have errors, the errors can be dispersed to a plurality of error correction codes for error correction; the bipartite graph with the big girth is adopted to realize the correspondence of layering and block codes, so that very many oligonucleotide molecules can form a whole, and the error correction capability and the DNA storage reliability are improved. Furthermore, the artificial base pairs are included, the storage capacity of a single base is increased, each molecule can be decomposed into more layers, and the error correction performance and the storage efficiency are improved. The method has the advantages that artificial bases can be contained, so that the storage density of single bases is improved; the storage reliability is ensured through layering and bipartite graph-based interleaving; the invention provides a high-reliability and high-density DNA storage coding method.

The invention provides a kit for extracting stable bacterial genome DNA by a paramagnetic particle method and an extraction method thereof. The kit comprises a lysis reagent group for lysing and releasing bacterial genome DNA and an extraction reagent group for extracting the genome DNA, wherein the lysis reagent group consists of a buffer solution and a lysis solution; the extraction reagent group consists of a paramagnetic particle binding solution, a washing solution and an eluent; and the paramagnetic particle binding solution consists of sodium salt sodium citrate or sodium acetate, a reducing agent dithiothreitol, guanidine hydrochloride and a paramagnetic particle suspension. The raw materials are reasonably compounded to obtain the optimal proportion and the combined extraction conditions of a paramagnetic particle binding solution compounding system, and the extraction method is optimized, so that the extracted bacterial genome DNA has high purity and complete fragments, is difficult to degrade, has stability far superior to that of the commercially available kit, and has remarkable advantages in subsequent experiments such as DNA storage, hybridization, PCR and the like; and meanwhile, the reagents are nontoxic and have small environmental pollution.

The invention relates to an image reconstruction method and device. The method comprises the following steps: obtaining a DNA base sequence corresponding to a compressed image stored by utilizing DNA, wherein the DNA base sequence comprises a plurality of DNA bases and / or base combinations; decompressing the DNA base sequence into first image data according to a corresponding relationship between DNA bases and / or base combinations and a decompression value; and inputting the first image data into an image reconstruction model, and outputting second image data through the image reconstruction model. According to the method, the second image with higher pixel density, finer image quality and more details is reconstructed from the first image data, so that the requirement of higher image quality is met, and a powerful technical guarantee is provided for wide application of DNA image storage.

A storage display vessel for biological material, including a block of, for example, glass or plastic, having at least one transparent area, and a void defining a storage area in the transparent area of the block communicating with an outside surface of the block for receiving biological material therein for storage. A plug is provided, and is adapted for sealing the storage area and the biological material therein from communication with the outside surface of the block. At least one three-dimensional internal visual indicator is carried by the block indicating that the block contains a biological material.

The invention discloses a quality control product of a stably-stored lung cancer methylated gene detection kit. The quality control product comprises a positive reference product, a negative referenceproduct and a detection limit reference product which each comprise corresponding DNA and a corresponding DNA storage solution. The DNA storage solutions can effectively prevent the degradation of free DNA, provide a stable environment for the DNA, and prolong the preservation time of the quality control product; the DNA quality control product containing the DNA storage solutions has the advantages of being low in preparing cost, stable in performance and easy to store; the operations of personnel, the states of instruments, the effectiveness of the kit and the like can be effectively monitored, and the accuracy and reliability of the detection kit are greatly improved.

The invention discloses a DNA storage coding optimization method based on an improved Harris Hawk algorithm. The method specifically comprises the following steps: in order to construct DNA coding sequences meeting a combination constraint condition as many as possible, firstly, initializing a certain number of random DNA sequences as an initial population, and calculating and sequencing fitness values (the sum of Hamming distances) of the population; secondly, updating the initial group by using different strategies of the improved Harris Hawk algorithm, wherein the added nonlinear convergence factor can maintain the smooth transition of the algorithm exploration and development process, a random reverse learning strategy is helpful to prevent the population from falling into the local optimum, and the updated DNA coding sequence with high fitness is selected by an elite selection mechanism; screening the sequence updated each time through a combined constraint condition to judge whether to add an alternative solution set or not; and finally, outputting an optimal DNA coding sequence set. According to the method, the lower bound of the DNA constraint coding sequence set is obviously improved.

The invention discloses a method for storing a character dot matrix by using a DNA character code. The method comprises the following steps of: expressing characters or character dot matrixes by usinga DNA character code with the length of N basic groups, wherein the character code is in the form of N + 1, the last bit of the character code is a check code obtained through statistical calculationaccording to the previous N basic groups, the obtained character code is added to the beginning and the end of the corresponding character or character dot matrix and added to the dictionary, and character dot matrix storage is directly carried out by using the character code with the check code generated by the method; by the adoption of the character dot matrix storage method, the defect that acharacter dot matrix DNA storage method is prone to generating large-segment repeated DNA sequences can be overcome, meanwhile, the short-length DNA character codes are adopted to replace character dot matrixes to record character information, and the storage density is greatly improved.

The invention is suitable for the technical field of storage, and particularly relates to a coding method, a decoding method, devices, terminal equipment and a readable storage medium. The coding method comprises the following steps: acquiring an initial base sequence of to-be-coded data; then grouping the initial base sequences to obtain at least one group of intermediate base sequences corresponding to the initial base sequences, and replacing the intermediate base sequences by using a first preset base sequence of which the proportion of G to C meets a preset condition to obtain a coding file corresponding to the to-be-coded data. According to the coding method provided by the embodiment of the invention, the GC proportion in the coding file can meet the requirement, the cost of DNA sequence synthesis and sequencing and the error probability are reduced, the cost of DNA storage is reduced, and the application scale of DNA storage is expanded.

A family genetic history storage system for storing DNA information on an identification certificate includes a selected area for attaching an identification indicators with at least one DNA blood sample storage area. It includes a means for storing the blood sample for future use and analysis while protecting it from environmental contamination. The user may be provided with DNA blood sample filter paper and a disposable lancet to help the user to easily obtain a blood sample. In another embodiment at least one DNA storage container is provided to allow for storage of biological cell material samples such as hair or fingernails. The certificate also may include an official certification proof area where official provenance is attached to certify authenticity.

A DNA birth certificate has a DNA sample portion to securely hold a biological sample for use in positively identifying an individual. It can be used to keep track of babies to prevent hospital mix-ups or in child stealing cases. Additionally, the certificate can be an ID or even a driver's license. The DNA sample portion holds one or more containers that allow the user to place a biological sample such as a hair or nail clipping within to provide the DNA if needed. In another embodiment, a DNA sample storage card known as an FTA© CLASSIC CARD©, a patented DNA storage card, is used to store the DNA information. Other biometrics such as finger or footprints, hair samples, nail clippings, etc. may also be stored on the DNA certificate.

The invention relates to the technical field of data storage, and discloses a data storage method, electronic equipment and a computer readable storage medium. The method comprises the steps of obtaining incremental information corresponding to target data; wherein the target data is divided into a plurality of data blocks, the data blocks are stored in a DNA storage medium in a DNA storage mode,and the incremental information is generated based on operation on the target data; and storing the incremental information, and establishing an association relationship with a corresponding data block in the plurality of data blocks. Through the mode, the data updating efficiency in the DNA storage medium can be improved, and the storage cost is reduced.

The invention discloses a damping factor-based DNA storage coding optimization method of a multivariate universe algorithm, which specifically comprises the following steps of: constructing an optimalDNA coding sequence meeting a combination constraint condition, firstly, constructing a certain number of DNA sequences as an initial population, and evaluating and sequencing the fitness of the population; secondly, optimizing the obtained DNA coding sequence by using an updating formula containing a damping factor and Levy flight search to obtain a DNA storage coding sequence with relatively high fitness; then, judging whether to add an alternative solution set or not according to constraints through constraint comparison; and finally, outputting the optimal DNA storage coding sequence. Bymeans of the method, DNA storage coding sequences with the optimal number can be searched out.