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Freezing method of biological tissue sample and biological tissue freezing container

A technology for biological tissues and cryopreservation containers, which is applied in the field of medical devices, can solve problems such as difficult removal and identification, and achieve the effects of reducing damage and good water absorption

Active Publication Date: 2017-12-01
FUDAN UNIV SHANGHAI CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the above problems, the main purpose of the present invention is to provide a method for cryopreservation of biological tissue. On the one hand, this method can solve the problem that the biological tissue is not easy to be identified or taken out due to the fact that the biological tissue is frozen on the inner wall of the cryopreservation container. On the other hand, it can avoid repeated freezing and thawing of biological tissues when making frozen sections, which is beneficial to the protection of the quality of tissue samples

Method used

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  • Freezing method of biological tissue sample and biological tissue freezing container
  • Freezing method of biological tissue sample and biological tissue freezing container
  • Freezing method of biological tissue sample and biological tissue freezing container

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A cryopreservation method for biological tissue, comprising the steps of:

[0047] (1), separating the required biological tissue from the living body;

[0048] (2) Process the freshly separated biological tissue into a suitable size, place it on the carrier sheet, and make the biological tissue adhere to the surface of the carrier sheet;

[0049] (3) Place the carrier sheet adhered to the biological tissue in the biological tissue cryopreservation container along the long axis direction of the biological tissue cryopreservation container;

[0050] (4) Seal the biological tissue cryopreservation container, and place it in a suitable direction at a low temperature for cryopreservation.

[0051] Wherein, in step (1), the biological tissue can be various human tissues or animal tissues, including surgically removed tissues and experimental animal tissues. According to the needs of the experiment, these biological tissues can be tumor tissues and normal tissues matched with...

Embodiment 2

[0057] like figure 1 and figure 2 As shown, this embodiment provides a biological tissue cryopreservation container 1 , which includes: a container body 2 , a sealing cover 3 and a carrier sheet 4 .

[0058] The container body 2 is in the shape of a hollow cylinder, and is detachably connected with the sealing cover 3 . When the container body 2 and the sealing cover 3 are connected together, the two form a closed space to prevent external substances (such as cryopreservation liquid, etc.) from entering the space during freezing, thereby avoiding the space from being polluted.

[0059] The container body 2 and the sealing cap 3 can be connected through various sealing methods such as threaded connection and snap connection. If the container body 2 and the sealing cover 3 form a sealed connection by being threaded, then an external thread can be established on the outer surface of the container body 2, and an internal thread can be established on the inner surface of the sea...

Embodiment 3

[0070] The container body of the biological tissue cryopreservation container in the second embodiment is in the shape of a hollow cylinder. In fact, the container body of the biological tissue cryopreservation container is not limited to the above-mentioned shape, as long as it can facilitate the freezing of biological tissue and prevent the cryopreservation liquid from entering the interior of the container body.

[0071] like Figure 5 As shown, the outside of the bottom of the container body 6 of the biological tissue cryopreservation container can also be provided with a support frame 7 to more securely support the entire biological tissue cryopreservation container, and other structures are the same as in the second embodiment.

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Abstract

The invention provides a cryopreserving method for a biological tissue sample and a biological tissue cryopreserving container. The cryopreserving method comprises the steps that the biological tissue is arranged on the surface of a bearing piece, the bearing piece to which the biological tissue is attached is placed in the biological tissue cryopreserving container in the long axis direction of the biological tissue cryopreserving container, and the biological tissue cryopreserving container is sealed and frozen at the low temperature; and the cryopreserving container comprises a container body, a sealing cover and at least on bearing piece used for bearing the separated biological tissue and separably arranged in the container body. According to the cryopreserving method, the problems that due to the fact that the biological tissue is prone to being cryopreserving on the inner wall of the biological tissue cryopreserving container, the biological tissue is not prone to being recognized or being taken out or being damaged after being taken out can be solved, the situation of multigelation when the biological tissue is made into cryopreserved sections can be avoided, and the protection of the sample quality (such as the contained RNA, proteins, antigens, antibodies and other biomacromolecules) of the biological tissue is facilitated.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and relates to a biological tissue cryopreservation method and a biological tissue cryopreservation container. Background technique [0002] In the process of studying the life activities of organisms, isolated biological tissues are often used as experimental objects for research. These tissues include normal tissues or tumor tissues. [0003] In order to conduct more in-depth research on the structural or biochemical properties of biological tissues, it is often necessary to store biological tissues at low temperature (for example, in liquid nitrogen), and then perform these biological tissues at different times after cryopreservation. Research. Cryopreservation containers are often used to perform the function of cryopreservation of biological tissues. [0004] In order to make the cryopreservation container not only maintain the low temperature storage function for a long time but a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B65D81/18B65D25/10A01N1/02
CPCA01N1/0263B65D25/10B65D81/18B65D2525/00B65D2581/00
Inventor 杜祥
Owner FUDAN UNIV SHANGHAI CANCER CENT
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