NAT2 gene polymorphism fluorescence PCR melting curve detection kit
A technology of gene polymorphism and detection kit, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high cost of gene chip technology, long detection cycle of sequencing method, contamination of false positive results, etc. Achieve the effects of avoiding repeated freezing and thawing, short detection time, and easy results
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[0035] A NAT2 gene polymorphism fluorescence PCR melting curve detection kit, which is a dry kit (no solvent exists, each reagent and nucleotide is dry powder), including a first probe, a second probe, a A third probe, a fourth probe, a forward primer and a reverse primer, specifically, the single-tube reaction system includes: 1×PCR buffer, 2U Taq DNA polymerase, 200μM dNTP, 4.5mM MgCl 2 , forward primer 4pmol, reverse primer 40pmol, first probe 2.5pmol, second probe 5pmol, third probe 7.5pmol and fourth probe 8.75pmol; wherein, 1×PCR buffer includes: Tris-HClpH8 .5 67mM, (NH4) 2 SO 4 16 mM and 0.1% (w / v) Tween.
[0036] The first probe is shown in SEQ ID NO 01, its 5' ends are respectively labeled with a fluorescent group CY5, and its 3' ends are both marked with a quencher group BHQ2.
[0037] The second probe is shown in SEQ ID NO 02, its 5' ends are respectively labeled with a fluorescent group HEX, and its 3' ends are both marked with a quencher group BHQ1.
[0038]...
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