The invention discloses a construction method of a human
nerve stem cell bank. The method comprises the following steps: carrying out human
urine separation to obtain
human kidney epithelial cells, subculturing the
human kidney epithelial cells, partially cryopreserving the
human kidney epithelial cells, inducing the subcultured human
kidney epithelial cells to form nerve stem cells, separating the nerve stem cells obtained after induction, carrying out amplification culturing, identifying the nerve stem cells, cryopreserving the confirmed nerve stem cells, coding and warehousing. The method avoids the use of
fetal bovine serum in the whole process, eliminates the introduction of foreign proteins, and reduces hidden troubles; the method allows the successfully
induced stem cells with activity to be preserved in order to form the
bank, and the stem cells can be provided for clinic use within 3d; and a
cryopreservation mode in the method is characterized in that nerve bulbs with a certain size are cryopreserved, a protection agent is introduced step by step in the
cryopreservation process, and the
cooling rate is set in grading, so recovered nerve stem cells still have
high cell viability and activity, and have significantly higher than
cell viability and activity than the recovered nerve stem cells preserved through current routine slow low-temperature
cryopreservation methods.