37 results about "Vicugna pacos" patented technology

The invention discloses a composite accompanying environment-friendly imitation goose down thermal material and a method for producing the same.The composite accompanying environment-friendly imitation goose down thermal material is prepared from PLA (polylactic acid) melt-blown superfine short fibers and a type or a plurality of types of cashmere, camel hair, alpaca wool and rabbit hair fibers.The surface density of the composite accompanying environment-friendly imitation goose down thermal material is 50-300 g/m<2>, the thickness of the composite accompanying environment-friendly imitation goose down thermal material is 3-30 mm, a CLO value of the composite accompanying environment-friendly imitation goose down thermal material is 0.8-3, the warmth retention rate of the composite accompanying environment-friendly imitation goose down thermal material is 50-85%, and a thermal resistance value R of the composite accompanying environment-friendly imitation goose down thermal material is 0.5-3.The composite accompanying environment-friendly imitation goose down thermal material and the method have the advantages that shortcomings of bulkiness, easiness in deformation due to water washing, down piercing and the like of the traditional down feather can be effectively overcome; existing melt-blowing equipment is modified, so that the light, thin, environment-friendly and efficient imitation goose down thermal material can be developed and is wide in service range, and development of the thermal material industry can be effectively promoted.

The invention discloses a canine parvovirus single-domain antibody, and a preparation method and an application thereof. The canine parvovirus single-domain antibody has a nucleotide sequence as shown in SEQIDNO.1. The preparation method comprises the steps: immunizing an alpaca by a canine parvovirus inactivated vaccine, and taking peripheral blood to obtain alpaca anti-canine parvovirus serum; extracting RNA in the peripheral blood, and carrying out reverse transcription to generate cDNA; utilizing specific primers for amplification, and purifying a VHH fragment; connecting the VHH fragment with a phagemid vector, transforming TG1, and constructing a canine parvovirus single-domain antibody library; and enriching the antibody library by utilizing a canine parvovirus structural protein VP2, introducing into an expression bacterial strain E.coil HB2151, and utilizing IPTG to induce the canine parvovirus single-domain antibody VHH having the biological activity to be expressed. The prepared canine parvovirus single-domain antibody has the detection activity of 15 [mu]g/ml and the detection sensitivity of 250 ng/ml, and can be used as drugs applied for treating dogs with canine parvovirus disease.

The invention provides a manufacturing technology for wool/alpaca/Persian nylon blending straight-forward double-faced wool goods. The manufacturing technology for the straight-forward double-faced wool goods comprises the steps that Australian wool tops, mercerizing wool tops, alpaca wool tops, and Persia nylon fiber rods are dyed separately and sufficiently mixed to be uniform, and the mule spinning technology is adopted for spinning 11/1 yarn; the spun yarn is woven on a rapier loom, the total warp number is 5,600-5,700, the acting reed width is 185-195 cm, the reed number/thread number is33/9, the acting reed weft density is 260-270/10 cm, and the fabric gray weight is 980-1,000 g/m<2>; the woven gray fabric is subjected to hemming, milling, scouring, dehydrating, drying, decatizing,dry beating, drying, ironing, shearing and decatizing to obtain the finished product.

Belonging to the field of molecular biology, the invention in particular relates to an anti-idiotypic nano antibody based zearalenone green immunoassay method. According to the invention, the zearalenone monoclonal antibody 2D3 is employed to immunize alpaca, and the leukocyte total RNA is extracted to construct a phage displayed nano antibody library; the 2D3 antibody is adopted as the target, four rounds of affinity enrichment panning are conducted to lower the coating concentration and competitive elution concentration round by round, and a positive phage zearalenone anti-idiotypic nano antibody with good specificity can be obtained. The invention employs the zearalenone anti-idiotypic nano antibody substitute zearalenone standard ELISA method to determine naturally polluted corn and feed samples, and performs comparison with the HPLC detection result, the two methods have good correlation, and R2 is 0.997, thus proving that the zearalenone anti-idiotypic nano antibody substitute standard method has accurate and reliable result and is an effective and feasible green immunoassay method.

The invention discloses a nanometer antibody 2018AFB-N11 having high specific recognition of Aflatoxins B1 and an application of the nanometer antibody 2018AFB-N11. The Aflatoxins B1 are used for complete antigen immunity of alpacas, RNA in blood of the alpacas after immunity is extracted, a one-step method RT-PCR system is used for amplification of alpaca heavy chain antibody variable region VHHgenes, and after connection with a pComb3X carrier, colon bacillus is transformed to obtain a high-quality nanometer antibody gene pool, the Aflatoxins B1 complete antigen is coated to coated wells, aphage display nanometer antibody gene pool is screened, a nanometer antibody gene capable of specifically recognizing Aflatoxins B1is obtained, then the gene is transformed to colon bacillus, and a nanometer antibody strain which can be efficiently expressed in the colon bacillus is established. The Aflatoxin B1 nanometer antibody 2018AFB-N11 is high in sensitivity and good in specificity, and the 50% inhibition concentration IC 50 of the Aflatoxins B1 is 1.26ng/mL. The cross reaction rate with Aflatoxins B2, G1, G2 and M1 is smaller than 0.1%. The nanometer antibody 2018AFB-N11 can be used for research and development of a detection reagent for high specific recognition of Aflatoxins B1.

The invention provides a method for rapidly acquiring a nano antibody by using a high-flux sequencing technique. With the combination of a flow cytometry separation technique and a high-flux sequencing technique, the method comprises the following steps: acquiring alpaca peripheral blood mononuclear cells (PBMC) which are immunized or unimmunized by using a specific protein W; acquiring PBMCs which are specified for the specific protein W by using a flow cytometry separation method; extracting total RNA (Ribonucleic Acid) of unimmunized, immunized and separated PBMCs, and performing inverse transcription into cDNA (Complementary Deoxyribonucleic Acid); by taking the cDNA as a template, performing two-step method PCR (Polymerase Chain Reaction) amplification so as to obtain DNA fragments of a specific protein W resistant nano antibody; establishing a DNA library of the DNA fragments of the nano antibody, and performing high-flux sequencing analysis; and analyzing the abundance of the DNA fragments of the nano antibody; establishing nucleotide sequences of the DNA fragments of the nano antibody into an expression vector, and expressing the DNA fragments of the nano antibody in appropriate hosts.

The invention belongs to the field of molecular biology, in particular to relates to an ochratoxin A anti-idiotypic nano-antibody and a preparation method thereof, wherein the amino acid sequence of ochratoxin A anti-idiotypic nano-antibody is shown in SED ID No:1. The antibody uses ochratoxin A monoclonal antibody to immunize alpaca, and constructs a bacteriophage display nanometer antibody library by extracting the total RNA of its white blood cells, and the library capacity is high and the diversity is good; taking ochratoxin A monoclonal antibody as the target, three round of affinity enrichment and eluting are used to reduce the coating concentration and competitive eluting concentration one by one, and the positive bacteriophage ochratoxin A anti-idiotypic nano-antibody gene is transformed into the expressed strain; after induction, purification and identification, the protein form nano-antibody VHH2-24. With excellent performance is obtained.

The invention relates to the technical field of biological nano materials, in particular to a nano antibody of Zika virus, and a preparation method and application thereof. An alpaca is immunized withvirus particles of Zika virus and then cloned; cloned DNA sequences are inserted in appropriate positions of a phage coat protein structural gene, and a sequence with higher affinity is selected through affinity elutriation; finally, monoclonal ELISA (enzyme-linked immunosorbent assay) identification is carried out, and antibody expression and purification can be carried out to obtain the nano antibody of Zika virus. The nano antibody of Zika virus is obtained by immunizing the alpaca and through a phage display technology, and thus the nano antibody can be highly expressed in prokaryotic oreukaryotic systems, has strong specificity and high affinity, and is more suitable for specific serological detection of Zika virus.

The invention discloses an alpaca opener. The alpaca opener comprises a feeding mechanism, an opening mechanism and a stripping mechanism. The opening mechanism comprises a pair of feeding rollers, a first licker-in, a second licker-in, a cylinder and a doffer, all of which are sequentially arranged from back to front. The feeding rollers slightly hold alpaca fibers, so that damage can be relieved. The feeding rollers are of needle loop type structures, and damage to the alpaca fibers can be further relieved. The first licker-in and the second licker-in form a double-licker-in structure, the speed ratio of the fibers in the transfer opening process can be reduced, alpaca is slowly opened, and the opening effect is better. In addition, only three work and wool stripping roller sets are arranged on the surface of the cylinder, the number is small, and the damage to the alpaca fibers can be effectively relieved. Through combined design of the feeding roller and double-linker-in structures and a small number of work and wool stripping roller sets, damage to the alpaca fibers in the opening process can be effectively relieved.

The invention discloses a piece dyeing technique of alpaca fiber, wool and nylon. The technique includes steps of step one: pretreatment of alpaca fiber; step two, piece dyeing of alpaca fiber, wool and nylon; the piece dyeing process mainly includes (1), sewing alpaca fiber, wool and nylon to be a fabric and then placing in a dye cylinder; adding pretreatment agent; (2), heating the dye cylinderto 80 DEG C at the heating rate of 1 DEG C/min and then preserving temperature for 20 minutes; cleaning the fabric; (3), adding dye leveler, penetrant, blocking agent and dye; running the dyeing cylinder for 25 minutes, and heating it to 60 DEG C at the heating rate of 1.5 DEG C/min, and preserving temperature for 15 minutes; heating to 75 DEG C at the heating rate of 1.5 DEG C/min, preserving temperature for 20 minutes and then fixing the dye; (4), heating the dye cylinder to 100 DEG C at the heating rate of 1 DEG C/min; if tint is dyed, preserving temperature for 70 minutes and then coolingand cleaning; if deep color is dyed, preserving temperature for 100 minutes, and dyeing deep color and then performing soaping treatment. The piece dyeing technique can perform piece dyeing on a mixedfabric of the alpaca fiber, wool and nylon.

The invention discloses an anti-human amyloid beta-peptide nano antibody (Abeta) and application thereof. A bacteriophage display technique is utilized to carry out multi-screening on an alpaca bacteriophage antibody library to enrich the Abeta-specific bacteriophage; the bacteriophage is cultured to prepare the antibody; identification is carried out to obtain a positive clone; sequencing is carried out to obtain a corresponding coding sequence; and expression is carried out in Escherichia coli to obtain a soluble antibody segment. The antibody disclosed by the invention has the amino acid sequence disclosed as SEQ ID NO.8. The antibody disclosed by the invention has the specific combining capacity with Abeta, and can be used for preparing Abeta-targeted drugs for reagents for disease therapy and diagnosis.

The invention discloses a manufacturing method of alpaca wool/superfine wool 50/50 sirospun blended yarn and belongs to the technical field of spinning, aiming to provides a alpaca wool and superfinewool blended yarn manufacturing method. According to the method, with the proportion of 50/50 of alpaca wool to superfine wool, yarn product with high quality, soft and smooth touch and good elasticity can be manufactured; the blended yarn manufactured by the method has both advantages of the two raw materials, safety and added value of products of the yarn and its fabric are improved with combination of the two raw materials, and the yarn is unique, novel and fashionable. Particularly, the method includes determining the optimal spinning process route, process configuration and process parameter , utilizing the siro-spinning technology to reduce hairiness of the yarn, and determining the gauge length of sirospun feed bellmouths for spinning of the 30tex yarn to be 4mm.

The invention discloses small interfering ribonucleic acid (siRNA) capable of interfering alpaca melanin cell CDK5 gene expression. The siRNA has positive-sense strand and antisense strand sequences as shown in Seq ID No. 1 and Seq ID No. 2; a pENTR/U6 short hairpin RNA (shRNA) expression plasmid capable of interfering the alpaca melanin cell CDK5 gene expression is also established. The biological effect of the pENTR/U6 (shRNA) expression plasmid shows a regulating function for alpaca melanin cell lines TYR and MC1R in a melanin cell line as well as an influence on phenotypic change of hair color of a mouse.

The invention discloses a wear-resistant mothproof ecological bacteriostatic down fiber, which is characterized by being prepared from the following raw materials by weight part: 25-35 of goose down, 5-7 of alpaca, 2-3 of soybean fiber, 3-5 of spun silk fiber, 4-6 of chitin, 0.3-0.5 of calcium chloride, 6-8 of vinyl triethoxysilane, 0.8-1.4 of nano-zinc oxide, 2.1-3.6 of celery oil, 2-4 of high wear-resistant carbon black, 185-195 of 1-butyl-3-methylimidazole chloride, and a proper amount of water. According to the invention, chitin molecules and down fiber are permanently combined through chemical modification, the chitin adsorbed on the surface of down fiber can combine with the saliva of microbial phospholipid and permeate into cells to adsorb anion carrying cytoplasm, undergoes flocculation and disturbs the normal physiological activities of cells so as to kill cells, and the antibacterial properties are thorough. The ecological bacteriostatic down fiber prepared by the invention has the advantages of wear resistance and mothproofness, strong antibacterial performance, and washing resistance, etc.

A temp controllable sperm taking table with pseudo-cervix structure for alpaca is composed of a table body, a pseudo-vagina and its holder on said table body, and a sperm collecting tube. Said pseudo-vagina consists of casing, heating and temp controlling unit, water filling and pressurizing valve with hydraulic pulse device, and an inner tube with pseudo-cervix structure.