48 results about "Trypsin activity" patented technology

The instant invention provides a method of treating an animal suffering a disease characterized by excessive apoptosis by administering a therapeutically effective amount of at least one serine protease inhibitor and thereafter monitoring a decrease in apoptosis. The inhibitor of the invention includes α1-antitrypsin or an α1-antitrypsin-like agent, including, but not limited to oxidation-resistant variants of α1-antitrypsin, and peptoids with antitrypsin activity. The diseases treatable by the invention include cancer, autoimmune disease, sepsis neurodegenerative disease, myocardial infarction, stroke, ischemia-reperfusion injury, toxin induced liver injury and AIDS. The method of the invention is also suitable for the prevention or amelioration of diseases characterized by excessive apoptosis.

A method for determining the risk of an individual of suffering from inflammation, opportunistic infection or disruption of immunoglobulin metabolism, comprising (a) determining the level of fragmentation or modification of Fc function of immunoglobulins in a sample taken from the individual and (b) determining thereby the risk of inflammation, impaired immune response or opportunistic infection. The invention also provides the use of a trypsin inhibitor in the manufacture of a medicament for use in the treatment or prevention of a disorder associated with elevated trypsin activity which is correlated with IgG fragmentation or modification.

The invention relates to a method to make trypsin activity restraining peptide by compound enzyme multiple step directional enzymolyzing oysters. The feather is that: adding oyster meat into 2-4 times weight Tris-Hcl buffer solution and pulping; according to 550-650U / g meat ratio to add flavor alkali protease, whisking in constant temperature and enzymolyzing for 1-2 hours under the temperature between 45-55 degree centigrade, then putting out enzyme, and cooling; using Hcl to adjust the PH value to 5-6, adding bromelain according to 650-750U / g meat ratio, whisking, enzymolyzing for 1-2 hours under 45-55 degree centigrade, then putting out enzyme, cooing, centrifuging, gathering the supernatant filtering, separating and purifying, then freezing out. The product has high activity and the restrain rate to the activity of commodity trypsin could reach 67%.

The invention relates to an amidino guanido substituted aromatic heterocyclic compound represented by the general formula (I), wherein, R1, R2 can represent same or different groups, and can substitute the group at any position of the hetero-aromatic ring, can be represented as: -O-R3, -S-R3, -COOR3, -COR3, -O-COR3 and -NH-COR3, m represents a natural number such as 0,1or 2; R3 represents a hydrogen atom, linear alkyl or branched alkyl, substituted or non-substituted aromatic group or aromatic heterocyclic ring; R4 and R5 represent a hydrogen atom, linear alkyl or branched alkyl, aromatic group or aromatic heterocyclic ring; A ring represent a cycloaliphatic ring, aromatic ring, aromatic heterocyclic ring or fused heterocycle; X and Y represent carbon, nitrogen, oxygen, sulfur and the like and can be any position on the ring; B represents -COO-, -CONH-, -COS-, -CSO-, -CSNH-, -CSS-, -OCO-, -NHCO-, -SCO-, -OCS-, -NHCS-, -SCS- or the like, and n represents a natural number such as 0,1or 2. The compound disclosed in the invention has stable property, can inhibit tryptic activity proved by enzymatic activity experiments, and can be used for preparing medicines for treating acute or chronic pancreatitis.

The invention discloses a fluorescent substrate for detecting the activity of trypsin acting on Cry1A protoxin and an application of the fluorescent substrate. The fluorescent substrate consists of fluorescence quenching group-Gly-GLy-Glu-Arg-Ile-Glu-Thr-Gly-Glu-fluorescence group. The invention also discloses the application of the fluorescent polypeptide substrate R28 in detecting the activity of insect midgut trypsin which directly acts on activated bacillus thuringiensis Cry1A protoxin. Compared with a conventional protease detection method, the fluorescent substrate disclosed by the invention has definite specificity and pertinence for detecting the activity of the insect midgut trypsin which directly acts on the activated bacillus thuringiensis Cry1A protoxin and detecting the activity of the trypsin which only acts on the corresponding fluorescent substrate R28, and intense fluorescence signals are generated in an enzymatic hydrolysis reaction, so that the sensitivity of the detection is greatly improved.

The invention provides egg white powder with high foamability and a preparation method thereof and relates to reconstruction of whey protein structure and functional properties, belonging to the technical field of biological processing of foodstuffs. According to the invention, on the basis of preliminary work, enzymatic hydrolysis of lipase in advance and cooperative enzymatic hydrolysis of composite protease are utilized for treatment of egg white; total usage amount of lipase and composite protease is less than usage amount of individually used lipase or protease, and however, foamability and foam stability of egg white powder obtained by combined utilization of lipase and composite protease are higher than those of egg white powder obtained by individual utilization of lipase or composite protease; the egg white powder obtained in the invention can meet demands for high-grade products on the market, and the advantages of a simple process and high cost performance are achieved in the invention. According to the invention, the ratio of active usage amount of Aspergillus oryzae protease, papain and trypsin is determined to be 1:1:1; the usage amount and other technological parameters cooperatively allow egg white powder with high foamability to be obtained; egg white powder with high foamability provided in the invention enables the additional output value of eggs to be increased, lays a technical foundation for development and industrial production of special-purpose egg white powder products and increases economic benefits for enterprises.