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Solid phase-bound elastase-binding assay for the measurement of alpha1-antitrypsin activity

An elastase and protease inhibitor technology, which can be used in measurement devices, biological tests, and microbial determination/inspection, etc., which can solve the problem of quantitative limitation of active A1PI and the detection of excess reagents, such as elastase, etc.

Inactive Publication Date: 2014-04-23
BAXTER INT INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The standard test mode using the chromogenic method has a rather high limit for the quantification of active A1PI: about 3 μg / ml
Although the sensitivity of such test formats can be improved by using fluorogenic substrates for the detection of residual elastase, they are still limited by the fact that only the excess of one reaction reagent, such as elastase, and not the reaction product can be detected.

Method used

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  • Solid phase-bound elastase-binding assay for the measurement of alpha1-antitrypsin activity
  • Solid phase-bound elastase-binding assay for the measurement of alpha1-antitrypsin activity
  • Solid phase-bound elastase-binding assay for the measurement of alpha1-antitrypsin activity

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Embodiment Construction

[0007] In one aspect, the present invention relates to a method for measuring activity alpha in a sample 1 - A method for protease inhibitors (A1PI), comprising the steps of:

[0008] (a) binding elastase to a solid phase;

[0009] (b) incubating the solid phase with the sample;

[0010] (c) incubating the solid phase with the detection reagent bound to A1PI;

[0011] (d) determining the amount of detection reagent bound to the solid phase; and

[0012] (e) Determining the amount of active AlPI in the sample.

[0013] In this context, the term "measuring active A1PI in a sample" as used herein refers to determining the amount and / or concentration of active A1PI contained in a sample. Further, the term "active AlPI" as used herein refers to AlPI capable of displaying its natural function (ie, capable of binding and inactivating elastase and other serine proteases).

[0014] The elastase used in the method of the present invention is not particularly limited, and may be, fo...

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Abstract

The present invention relates to a method for the measurement of active alpha1-proteinase inhibitor (A1PI) in a sample. The method comprises the steps of binding elastase to a solid support, letting the A1PI contained in the sample bind to the solid phase-bound elastase, and detecting solid phase-bound A1PI with a detection reagent.

Description

technical field [0001] The invention relates to a method for measuring the activity alpha in a sample 1 - A method for a protease inhibitor (A1PI), comprising the steps of: binding elastase to a solid support such that A1PI contained in the sample binds to the elastase bound to the solid phase, and then detecting solid phase binding with a detection reagent The A1PI. Background technique [0002] human alpha 1 - Protease Inhibitor (A1PI; also known as: alpha-1-antitrypsin) at circulating concentrations at serum levels of 20 to 48 μM. The 51 kilodalton glycoprotein consists of 394 amino acids with three complex N-glycans linked to asparagine positions 46, 83 and 247. Different N-glycan branches and N-terminal truncations lead to characteristic isoelectric focusing patterns. In addition, a C-terminally truncated form of A1PI has been described recently that lacks the C-terminal lysine after cleavage by basic carboxypeptidases. A1PI is a serine protease inhibitor (serpins:...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/68
CPCG01N2333/966G01N33/53G01N33/68G01N2333/8125C12Q1/37
Inventor A·韦伯A·艾格美亚H-P·施瓦茨
Owner BAXTER INT INC
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