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Composition for dispersing biological tissue

A technology of biological tissue and composition, applied in the direction of microorganisms, tissue culture, biochemical equipment and methods, etc., can solve the problems of different effectiveness of anticancer agents, and achieve the effect of less tissue volume and less damage

Inactive Publication Date: 2016-06-22
KURASHIKI BOSEKI KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Although anticancer agents also act on normal cells and sometimes exhibit strong side effects, the effectiveness of anticancer agents is less than 50% except for some, and it is known that the degree of effectiveness of anticancer agents varies greatly depending on the patient

Method used

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  • Composition for dispersing biological tissue
  • Composition for dispersing biological tissue
  • Composition for dispersing biological tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] The collagenase activity of the compositions of Example 1 and Comparative Example 1 was measured by the following FALGPA decomposition activity measurement method.

[0115] 1. The method for the determination of FALGPA decomposition activity:

[0116] The following method is published on the Sigma-Aldrich website (http: / / www.sigmaaldrich.com / technical-documents / protocols / biology / enzymatic-assay-of-collagenase-using-n-3-2furylacryloyl-leu-gly-pro -ala.html).

[0117] (1) Abbreviation:

[0118] 【Table 2】

[0119] FALGPA

N-(3-[2-furyl]acryloyl)-Leu-Gly-Pro-Ala

FAL

N-(3[2-furyl]acryloyl)-Leu

Leu

Leucine

Gly

Glycine

Pro

proline

Ala

Alanine

[0120] (2) Principle:

[0121] When FALGPA was decomposed into FAL and Gly-Pro-Ala by the action of collagenase, the decrease variable of the 345 nm absorbance (A345nm) derived from FALGPA was measured, and the activity was calculated.

[0122] (3) Method: ...

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Abstract

The purpose of the present invention is to acquire a highly proliferative cell at a high efficiency from a sample derived from a biological tissue. Provided is a composition for dispersing a biological tissue, wherein a solution formulation of the composition has a collagenase activity of 0.30-10 U / mL, said collagenase activity being determined by a method for measuring FALGPA-decomposing activity, and a trypsin activity of 0-30 U / mL at a formulation concentration of the composition, said trypsin activity being determined by a method for measuring BAEE hydrolytic activity.

Description

technical field [0001] The present invention relates to compositions for dispersing biological tissue. In addition, the present invention relates to a method for evaluating the result of culture of cells embedded in a droplet bulk gel. Further, the present invention relates to methods for obtaining cells derived from biological tissues. In addition, the present invention relates to kits for carrying out the above methods. Background technique [0002] Although anticancer agents also act on normal cells and sometimes exhibit strong side effects, the effectiveness of anticancer agents is less than 50% except for some, and it is known that the degree of effectiveness of anticancer agents varies greatly among patients. Therefore, it is desired to evaluate whether the anticancer agent to be administered is effective to the cancer patient before the cancer patient is treated with the administration of the anticancer agent, to avoid unnecessary administration of an ineffective an...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12N5/09C12N5/10C12N9/50C12Q1/04
CPCC12N5/00C12N2509/00C12N9/6427C12N9/6489G01N33/5011C12N5/0693C12N9/6491C12N2503/02C12Y304/21004C12Y304/24007
Inventor 宫川功河村圣子小林昶运
Owner KURASHIKI BOSEKI KK
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