37 results about "Streptococcus fecalis" patented technology

An improved chemical wastewater processing material is made of, by weight, 9 parts to 17 parts of ferric sulfate, 6 parts to 13 parts of stearamide, 4 parts to 10 parts of stearate, 3 parts to 8 parts of photosynthetic bacteria, 7 parts to 9 parts of streptococcus faecium, 2.3 parts to 8 parts of tourmaline, 4 parts to 10 parts of zeolite, 6 parts to 14 parts of sodium dihydrogen phosphate, 1 part to 5 parts of gel, 6 parts to 13 parts of calendula, 4.5 parts to 7 parts of neodymium oxide, 1 part to 4 parts of tea polyphenol, 9 parts to 16 parts of nanometer titania, 7 parts to 12 parts of calcium oxide, 8 parts to 17 parts of gallic acid, 8 parts to 14 parts of lime and 6 parts to 13 parts of anionic polyacrylamide. The improved chemical wastewater processing material has the advantages that by means of the chemical wastewater processing material, heavy metal ions in solidification water can be neutralized, toxic substances can be reduced, and meanwhile the oxygen dissolving rate in water can be increased and the eutrophication phenomenon can be reduced.

The invention provides charcoal-based microbial compound bacterial powder, bacterial agent as well as a preparation method and application of the charcoal-based microbial compound bacterial agent andbelongs to the technical field of microbes. Microbes are adsorbed on a solid carrier to obtain the charcoal-based microbial compound bacterial agent; the solid carrier comprises charcoal and zeolite;microbes comprise bacillus and streptococcus faecalis; the mass ratio of the solid carrier and microbes is (40-60):1. The high porosity and high specific surface area of the charcoal are used and matched with high-adsorption material zeolite, the treatment performance of single microbial agent for livestock and poultry breeding sewage is improved, the pH of water is kept stable, nitrogen, phosphorus, organic pollutants and harmful heavy metal residues in wastewater are effectively reduced, and the pollution degree of surrounding water environments by livestock and poultry breeding sewage is relieved.

Disclosed herein are brown algae-derived fucoidan and in particular a method for degrading fucoidan to a low-molecular weight so that fucoidan as sparingly-degradable polysaccharide (M.W. 3,000,000 to 5,000,000) can be more efficiently extracted from brown algae and it can be absorbed in the human body. The method comprises preparing a fucoidan extract containing as much fucoidan as possible from brown algae selected from Hizikia fusiforme, Undaria pinnatifida, Laminaria saccharina, Ecklonia cava, Cladosiphon Okamuranus and Nemacystus decipiens, and low-molecularizating the fucoidan extract using Vibrio halioticoli (Accession No. KCTC 10998BP) and a probiotic mixture containing Streptococcus faecalis, Bacillus mesentericus, Clostridium butyricum, and glycosyltransferase thereof. According to the method, fucoidan can be efficiently degraded to a low molecular weight using microorganisms so that it can be absorbed in the human body. The fucoidan obtained realizes practical utilization of beneficial efficiencies thereof in a variety of fields, in particular, health foods, meals for patients (e.g., cancer patients), medicine, cosmetics, agriculture, fishing and stockbreeding.

The invention discloses a compound microbial fish fertilizer special for silver carp and a preparation method thereof. The compound microbial fish fertilizer special for silver carp comprises the following components in parts by weight: 0.5-0.8 part of silicate bacteria, 1-3 parts of streptococcus faecalis, 0.6-0.8 part of trichoderma sp, 2-5 parts of rhodopseudomonas palustris, 0.3-0.5 part of saccharomycetes, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of effluent precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of waste of a pig meat slaughter house, 60-65 parts of aquatic plants, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of Vitamin B12, 1-3 parts of rheum officinale, 2-6 parts of Astragalus membranaceus and 4-7 parts of medicated leaven. The fertilizer disclosed by the invention can promote silver carp to grow quickly, the EPA content of fish is increased, the fish diseases are reduced, and the number of dead fish is reduced, and meanwhile, beneficial microorganisms in water are increased. Finally, both the quality and the weight of silver carp are improved.

The invention relates to a culture medium for enriching Streptococcus faecalis. The technical solution adopted is: a culture medium for enriching Streptococcus faecalis, characterized in that it consists of basal medium, salt solution, glucose and yeast extract; in the basal medium, add 3-7ml per 1000ml of basal medium salt solution, 15-20g of glucose and 5-15g of yeast extract; the salt solution is: 40g of MgSO4.7H2O, 2g of MnSO4.4H2O, 2g of FeSO4.7H2O and 2g of NaCl, with distilled water into 1000ml. The culture medium of the invention is used to cultivate the streptococcus faecalis, and the culture speed is fast and the cost is low. The shortcoming of the slow growth period and high cost of the streptococcus faecalis is made up for, the growth rate is increased, and the time for reaching the maximum growth amount is shortened by 3 to 5 hours.

The invention discloses a quadruple fluorescent PCR primer set for detecting four pathogenic bacteria in drinking water. The quadruple fluorescent PCR primer set comprises primers for coli groups, streptococcus faecalis, pseudomonas aeruginosa and clostridium perfringens, and is specifically shown as Seq. ID No. 1 to Seq. ID No. 8. The invention belongs to the technical field of gene engineering detection, mutual interference cannot be caused for each primer and each probe, amplification can be carried out on specific objective sequences only, a fluorescence signal is stimulated, non-objectivesequences are free of amplification or fluorescence signal, accurate detection for the four pathogenic bacteria in drinking water can be realized, and the quadruple fluorescent PCR primer set has theadvantages of high specificity, small standard error, short detection time, reagent cost saving and the like and is applicable for detecting the indexes of the pathogenic bacteria in the standard ofdrinking water.

The invention relates to a fertilizer, in particular to a microbial leaf surface fertilizer with pest preventing effect. The microbial leaf surface fertilizer with the pest preventing effect is prepared from the following raw materials: urea, magnesium sulfate, zinc sulfate, cotex phellodendri, alkyl glycoside, soybean protein, yeast extract, corn gliadin, ethanol with a volume fraction of 70-80%and streptococcus faecalis powder. The microbial leaf surface fertilizer with the pest preventing effect is directly sprayed on the leaf surface of vegetables, nutrients are directly absorbed from theleaf surface, the growth period is shortened, and the yield is improved; meanwhile, the nutrition of plants is enhanced and the own resistance to disease is improved, a protective film is formed on the leaf surface of the vegetables, insect pests are prevented from being attached to the vegetables, the cotex phellodendri component is contained, and the erosion of germs and insect pests on vegetables can be effectively prevented; and the metabolite produced by streptococcus faecalis destroys the growth environment required for the insect pests and the insect pests are further indirectly inhibited. According to the microbial leaf surface fertilizer with the pest preventing effect, the components of preventing germs and insect pests are plant extracts, are non-polluting to the environment, are more environmentally friendly and healthy, and food safety risks are eliminated.

The invention relates to a method for symbiotic culture of streptococcus faecalis and saccharomycetes. The technical scheme is that the optimum formulation of a culture medium is 2.4 g% peptone, 1.2-2.4 g% glucose, and 0.5-1 g% yeast powder, and the initial PH value is 7.0; the cultivation method comprises the following steps: the inoculum sizes of saccharomycetes seed solution and streptococcus faecalis seed solution are 2.5% and 1.0% respectively, the streptococcus faecalis seed solution is firstly inoculated in the culture medium to be cultured to the early exponential phase (about 4 hours), then the saccharomycetes seed solution is added, and at the temperature of 30 DEG C, the oscillating rotational speed is 180 rpm, the cultivation time is 20 hours, and next streptococcus faecalis and saccharomycetes are subjected to enlarge cultivation in a 5L fermentation tank, wherein the cultivation condition is that the throughput is 2.4 Lpm, the stirring rate is 100 rpm, the tank temperature is 30 DEG C, the broth content is 60%, and the cultivation time is 24 hours. The method is low in cost and stable in action effect, improves the number of compound bacteria, and enhances the action effect of a streptococcus faecalis and saccharomycetes complex microbial inoculant.

The present invention provides a triple vital bacteria composition, including: powder of Bifidobacterium longum, powder of Lactobacillus acidophilus, and powder of Streptococcus faecalis. The present invention further provides a method for preparing the above triple vital bacteria composition, as well as the protection agents, the fermenting culture medium and the seed culture medium used therein.

The invention discloses a pig feed additive containing probiotics. The pig feed additive containing probiotics includes the following raw materials in parts by weight: 10-18 parts of pine needle powder, 10-15 parts of kelp powder, 5-10 parts of orange peel powder, 5-10 parts of grape seed oil, 1-5 parts of bile acid, 1-5 parts of zinc sulfate, 1-5 parts of milkvetch root, 1-3 parts of fructooligosaccharides, 1-5 parts of lactobacillus acidophilus according to the mass ratio of lyophilized powder, and 1-5 parts of streptococcus faecalis. The pig feed additive provided by the invention uses thenatural forage, the bile acid, the zinc sulfate, the fructooligosaccharides, and two probiotics of the lactobacillus acidophilus and the streptococcus faecalis together, can promote the growth of piglets, enhance the body metabolism, enhance the body immunity, and improve the growth performance of livestock.

The invention discloses a biological preparation for balancing water quality for aquaculture. The biological preparation comprises the components of: sodium humate accounting for 49 percent, sodium thiosulfate accounting for 35 percent, biological flocculants accounting for 10 percent, bacillus subtilis accounting for 3 percent, streptococcus faecium accounting for 3 percent, wherein the bacillus subtilis is 3,000,000,000 / g and the streptococcus faecium is 3,000,000,000 / g. According to the biological preparation, the nutrition of a water body is balanced, the water quality is improved, the blue-green algae outburst is controlled, the growth of beneficial algae is activated, dissolved oxygen of the water body is increased, the content of beneficial microorganisms in the water body is increased, the occurrence of chronic nitrate and nitrous acid poison incidents of fishes can be prevented, the immune globulin and the antibody level of the fishes are improved, and the herd immunity of the fishes is improved; and by adding the streptococcus faecium, the true nutritive value of feed is improved, the concentration of ammoniacal nitrogen in excrements also can be reduced, and the pollution to the water quality is reduced.