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37 results about "Streptococcus fecalis" patented technology

Streptococcus faecalis appears in pairs, singly or in short chains that usually present in the large intestine which able to grow at temperature ranging from 10’C-42’C. It can ferment glycerol under aerobic and microaerophilic conditions. It is a diverse species which interacts with other several organisms.

Compound microbial fish fertilizer special for silver carp and preparation method thereof

The invention discloses a compound microbial fish fertilizer special for silver carp and a preparation method thereof. The compound microbial fish fertilizer special for silver carp comprises the following components in parts by weight: 0.5-0.8 part of silicate bacteria, 1-3 parts of streptococcus faecalis, 0.6-0.8 part of trichoderma sp, 2-5 parts of rhodopseudomonas palustris, 0.3-0.5 part of saccharomycetes, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of effluent precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of waste of a pig meat slaughter house, 60-65 parts of aquatic plants, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of Vitamin B12, 1-3 parts of rheum officinale, 2-6 parts of Astragalus membranaceus and 4-7 parts of medicated leaven. The fertilizer disclosed by the invention can promote silver carp to grow quickly, the EPA content of fish is increased, the fish diseases are reduced, and the number of dead fish is reduced, and meanwhile, beneficial microorganisms in water are increased. Finally, both the quality and the weight of silver carp are improved.
Owner:苏州市阳澄湖现代农业发展有限公司

Method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time

The invention provides a method for detecting fluorescent quantitative PCR of 12 pathogenic bacteria in real time at the same time, including the steps: collecting specific pathogenic gene or toxin gene of the target pathogen and using it as a target gene to design primers and probes so as to make the reaction conditions consistent; extracting a genome template of a sample to be detected; adding the template respectively into tubules equipped with different specific upstream and downstream primers and probes, and then adding the corresponding fluorescent quantitative PCR reagents; under the same cycle of fluorescent quantitative PCR, the corresponding primers and probes are used to detect the samples simultaneously, quickly and quantitatively in their respective reaction tubes. Easier, Quick and efficient, Twelve common pathogenic bacteria (Escherichia coli O157: H7, Listeria monocytogenes, Salmonella, Vibrio parahaemolyticus, Streptococcus betae, Yersinia enterocolitica, Streptococcusfaecalis, Shigella, Proteus mirabilis, Vibrio fluvialis, Campylobacter jejuni, Staphylococcus aureus) can be detected simultaneously in drinking water and food economically.
Owner:INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI

Composite biological living bacteria preparation and preparation technology thereof

The invention relates to a composite biological living bacteria preparation and a preparation method thereof. The total number of effective viable bacteria is not less than 108cfu / ml. The method includes: selecting optimal probiotics, carrying out supporting medium activation, performing enlarged culture and mixing, conducting culture by means of a mixed bacteria medium and a fermentation tank, carrying out filtration, concentration and proportioning so as to form the composite living bacteria preparation. The preparation contains the following bacteria with the total number of effective viable bacteria of: 22-25% of photosynthetic bacteria, 21-24% of yeast, 15-20% of bacillus subtilis, 15-20% of Pediococcus acidilactici, 12-16% of Streptococcus faecalis, and 12-16% of Lactobacillus acidophilus. The composite biological living bacteria preparation provided by the invention can be applied to agricultural cultivation, livestock and poultry breeding, aquaculture, feed adding, municipal sewage and domestic garbage treatment and other fields.
Owner:角志海

Green feed additive and preparation method thereof

The invention discloses a green feed additive and a preparation method thereof. The green feed additive is prepared by mixing the following components in portion by weight: 40 to 60 portions of cortex eucommiae extract, 10 to 20 portions of streptococcus faecalis powder, 30 to 40 portions of polysaccharide peptide powder and 8 to 20 portions of folium cortex eucommiae finemeal, wherein the cortex eucommiae extract at least contains 0.2 percent of chlorogenic acid; the polysaccharide peptide powder is prepared by concentrating, decoloring, precipitating with alcohol and drying the ferment liquid which is obtained by fermenting the streptococcus faecalis powder. The components of the invention are integrally applied, which can remarkably improve the function of an animal immune system, strengthen disease preventing ability of organism, and resist the intrusion of pathogen; the green feed additive contains virus resisting the immunodeficiency which can bidirectionally adjust the function of cellular immunity and greatly improve the survival rate of feeding animals; and under the conditions without hormone, the green feed additive can promote the growth and breed of animals, greatly improve the quality and taste of eggs and meat, and keep the meat quality more fresh and more close to wild taste.
Owner:SHANXI DAYU BIOLOGICAL ENG CO LTD

Alga lysing biological preparation and application thereof

The invention discloses an aga lysing biological preparation and an application thereof. The preparation is prepared through mixing Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, Streptococcus faecalis, photosynthetic bacteria, Sphingomonas sp., vitamin C, vitamin E and lactose. The above product has good stability and high efficiency, and consumes an extremely low amount of oxygen and rapidly degrades microcystis and other pollutants in culture water in the application process in order to effectively improve the environment of the water. The preparation is nontoxic and harmless to environment, people and animals, has no residuals, can substantially improve and purify the environment and the culture body and effectively avoid bursting of cyanobacterial bloom, and also can improve the immunity of aquatic product animals and reduce the incidence of diseases to make the ecologic system of the whole culture water tend to balance and go well.
Owner:HUAZHONG AGRI UNIV

Specific culture medium and quick test slip of streptococcus faecium

The invention discloses a chromogenic medium and a quick test slip of streptococcus faecium. The chromogenic medium of the streptococcus faecium comprises peptone, yeast powder, sodium chloride, lactose, maltose, TTC (Triphenyltetrazolium Chloride), a bacteriostatic agent and an indicator. The quick test slip is a stable microbial culture device comprising a base plate, a water absorbing gel layer, a culture medium layer and a covering film; the chromogenic medium is absorbed on the culture medium layer. The chromogenic medium of the streptococcus faecium is accurate in testing results, obvious in display of positive results, high in specificity, convenient to observe and capable of performing qualitative detection and quantitative analysis to the streptococcus faecium at the same time. According to the quick test slip of the streptococcus faecium, the conventional multi-step detection and identification process is simplified to be done in one step; and in addition, the test slip is small in size and convenient to carry, can sample on site at any time, is simple and convenient to operate, and provides scientific basis and guidance for the water quality safety.
Owner:GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD

Streptococcus faecalis powdering LAMP rapid detection kit, and use method thereof

The invention discloses a Streptococcus faecalis powdering LAMP rapid detection kit, and a rapid detection method based on the kit. The kit comprises a freeze-dried isothermal amplification agent, a re-dissolving solution, a positive contrast dry powder, a negative contrast substance, a colorimetric solution, a lysate and a sealing solution, and the freeze-dried isothermal amplification agent contains dNTPs, Bst DNA polymerase, an amplimer and a freeze-drying protection agent. The primer in the kit corresponds to eight segments of a Streptococcus faecalis specific gene sequence, the colorimetric solution is added after a reaction, and a negative and positive colorimetric difference is obvious. The kit has the advantages of strong specificity, high sensitivity, easiness in operation and simplicity in result judgment, and is suitable for the quality control and the safe and rapid detection of foods, beverages, water and other raw and auxiliary materials. The kit is convenient for normal temperature transportation, is simple and fast to use without special devices, reduces the cost, widens the application range of products, is suitable for onsite rapid detection and base remote region related detection, and has wide application prospect.
Owner:GUANGDONG HUANKAI BIOLOGICAL SCI & TECH CO LTD +1

Stichopus japonicus fertilizer, and preparation and application methods thereof

The invention relates to a Pseudobagrus fulvidraco feed. The invention is characterized in that the Pseudobagrus fulvidraco feed comprises the following raw materials in parts by weight: 0.5-0.8 part of silicate bacterium, 1-3 parts of Streptococcus faecalis, 0.6-0.8 part of Trichoderma, 2-5 parts of Rhodopseudomonas palustris, 0.3-0.5 part of microzyme, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of waste liquor precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of pork abattoir waste, 60-65 parts of aquatic, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of vitamin B12, 20-30 parts of pepper and the like. A comparison shows that the composite microbial fertilizer can promote quick growth of stichopus japonicus, increase the EPA content, reduce the fish diseases and the amount of fish dying of illness, and increase the beneficial microbes in the water body, thereby enhancing the final quality and grade.
Owner:SUZHOU YANGCHENGHU MODERN AGRI INDPARK SPECIAL AQUACULTURE

Improved chemical wastewater processing material

An improved chemical wastewater processing material is made of, by weight, 9 parts to 17 parts of ferric sulfate, 6 parts to 13 parts of stearamide, 4 parts to 10 parts of stearate, 3 parts to 8 parts of photosynthetic bacteria, 7 parts to 9 parts of streptococcus faecium, 2.3 parts to 8 parts of tourmaline, 4 parts to 10 parts of zeolite, 6 parts to 14 parts of sodium dihydrogen phosphate, 1 part to 5 parts of gel, 6 parts to 13 parts of calendula, 4.5 parts to 7 parts of neodymium oxide, 1 part to 4 parts of tea polyphenol, 9 parts to 16 parts of nanometer titania, 7 parts to 12 parts of calcium oxide, 8 parts to 17 parts of gallic acid, 8 parts to 14 parts of lime and 6 parts to 13 parts of anionic polyacrylamide. The improved chemical wastewater processing material has the advantages that by means of the chemical wastewater processing material, heavy metal ions in solidification water can be neutralized, toxic substances can be reduced, and meanwhile the oxygen dissolving rate in water can be increased and the eutrophication phenomenon can be reduced.
Owner:QINGDAO ENGAO MOTION CONTROL TECH

Mixed strain, strain vector and method capable of continuously degrading total ammonia nitrogen in black and odorous water in river

The invention discloses a mixed strain, strain vector and method capable of continuously degrading total ammonia nitrogen in black and odorous water in a river. The mixed strain consists of the following microorganisms: bacillus subtilis, bacillus licheniformis, photosynthetic bacteria, EM bacteria, streptococcus faecalis, lactobacillus, lactobacillus plantarum, lactobacillus acidophilus, nitrobacteria and denitrifying bacteria (all strains are commercially available); and moreover, all microorganisms contained in the mixed strain are domesticated into plant-sourced microorganisms, and the mixed strain is mixed with short-chain fatty acid. By adopting the synergism of various means, the total ammonia nitrogen contained in the black and odor water in the river is effectively treated.
Owner:钟华

Mixed strain and strain carrier as well as method for continuously degrading volatile phenol pollutants in river black and odorous water body

The invention discloses a mixed strain and a strain carrier as well as a method for continuously degrading volatile phenol pollutants in a river black and odorous water body, wherein the mixed strainis prepared from the following microorganisms: bacillus subtilis, bacillus licheniformis, EM fungi, streptococcus faecium, lactobacillus, lactobacillus plantarum and lactobacillus acidophilus; in addition, the microorganisms contained in the mixed strain are domesticated into plant-derived microorganisms, and short chain fatty acid is mixed in the mixed strain. According to the method disclosed bythe invention, by adopting multiple means to synergize, the volatile phenolic pollutants contained in the river black and odorous water body are effectively treated.
Owner:钟华

Charcoal-based microbial compound bacterial powder, bacterial agent as well as preparation method and application of charcoal-based microbial compound bacterial agent

The invention provides charcoal-based microbial compound bacterial powder, bacterial agent as well as a preparation method and application of the charcoal-based microbial compound bacterial agent andbelongs to the technical field of microbes. Microbes are adsorbed on a solid carrier to obtain the charcoal-based microbial compound bacterial agent; the solid carrier comprises charcoal and zeolite;microbes comprise bacillus and streptococcus faecalis; the mass ratio of the solid carrier and microbes is (40-60):1. The high porosity and high specific surface area of the charcoal are used and matched with high-adsorption material zeolite, the treatment performance of single microbial agent for livestock and poultry breeding sewage is improved, the pH of water is kept stable, nitrogen, phosphorus, organic pollutants and harmful heavy metal residues in wastewater are effectively reduced, and the pollution degree of surrounding water environments by livestock and poultry breeding sewage is relieved.
Owner:SHANGHAI ACAD OF AGRI SCI

Organic waste fermentation method

InactiveCN111943742ARapid fermentationEffective fermentation treatmentBio-organic fraction processingMicroorganism based treatmentBiotechnologyPichia pastoris
The invention discloses an organic waste fermentation method which comprises the following steps: stirring organic waste and compound bacteria, and stacking into a fermentation pile; covering the fermentation pile with a breathable film; introducing a gas containing oxygen into the fermentation heap; wherein the compound bacteria comprise bacillus subtilis, bacillus subtilis natto, bacillus brevis, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus delbrueckii, streptococcus faecalis, streptococcus lactis, streptococcus thermophilus, candida utilis, pichia pastoris, nitrifying bacteria, methane acidifying bacteria, sulfur reducing bacteria and photosynthetic bacteria. The organic waste fermentation method provided by the invention can quickly ferment, heat and evaporate water, and quickly decompose the generated harmful smell, thereby achieving the goal of innocent treatment of organic wastes.
Owner:陈宇宁 +1

Streptococcus faecium live bacteria preparation and preparation method thereof

The invention relates to a production method of a streptococcus faecium live bacteria preparation, belongs to the technical field of microorganisms, and in particular relates to a liquid anaerobic fermentation production method of the streptococcus faecium live bacteria preparation. The method comprises the following steps: by using streptococcus faecium as a fermenting culture, inoculating in an optimized liquid culture medium; cultivating for 16-20 hours at 40-42 DEG C; placing in a tank and measuring the number of live bacteria; absorbing the fermentation liquor by plant starch; and drying and crushing and packaging to prepare the streptococcus faecium live bacteria preparation with stable activity. The preparation provided by the invention has the advantages that high density fermentation of the streptococcus faecium live bacteria preparation can be completed by a common fermenting tank, and the preparation is simple to operate, stable in effect and low in energy consumption; devices such as a centrifugal machine and a freezer dryer are not used, so that the production cost is lowered and the production efficiency is improved, and the preparation is easy to popular and use.
Owner:GUANGZHOU GLAM BIOTECH

Nano emulsion for treating anaerobic infections of intestinal tracts of livestocks and preparation method of nano emulsion

The invention discloses a nano emulsion for treating anaerobic infections of intestinal tracts of livestocks. The nano emulsion comprises the following raw materials in percent by mass: 0.1-8.0 percent of linaloe oil, 0.1-7.0 percent of carvacrol, 0.01-0.7 percent of metronidazole, 19.0-35.0 percent of surfactant, 0-5.0 percent of cosurfactant and the balance of distilled water, wherein the sum of the mass percent of the raw materials is 100 percent. The nano emulsion has stronger inhibiting and killing functions to most of obligate anaerobes, and is mainly used for treating enteritidis caused by infections of anaerobic bacteria such as bacteroides, clostridium, aerogenesis bacillus, peptococcus, eubacteria and streptococcus faecalis, for the livestocks. The nano emulsion has the advantages of high bioavailability, remarkable antibacterial action and treatment effect, and wide market prospect in the field of veterinary medicines.
Owner:NORTHWEST A & F UNIV

Intestinal flora regulator for aquaculture and preparation method thereof

The invention discloses an intestinal flora regulator for aquaculture and a preparation method thereof. The intestinal flora regulator is prepared from the following components in parts by weight: 10 to 30 parts of saccharomycetes, 4 to 8 parts of bacillus licheniformis, 3 to 9 parts of lactobacillus acidophilus, 1 to 5 parts of streptococcus faecalis, 6 to 10 parts of bacillus, 7 to 9 parts of bifidobacterium, 3 to 7 parts of lactic streptococcus, 2 to 6 parts of lactobacillus plantarum, 5 to 9 parts of lactobacillus, 5 to 7 parts of bacillus coagulans and 3 to 9 parts of fat bacteria. The intestinal flora regulator for aquaculture, provided by the invention, is prepared from the saccharomycetes, the bacillus licheniformis, the lactobacillus acidophilus, the streptococcus faecalis, the bacillus, the bifidobacterium, the lactic streptococci, the lactobacillus plantarum, the lactobacillus, the bacillus coagulans and the fat bacteria, can ferment and convert undigested substances into energy, and prevents invasion of pathogenic bacteria and inhibits growth of the pathogenic bacteria; and meanwhile, the regulator is natural, free of toxic and side effects, free of residues, high in safety and beneficial to aquaculture.
Owner:福州一诺维生物科技有限公司

Compound microbial fish fertilizer special for silver carp and preparation method thereof

The invention discloses a compound microbial fish fertilizer special for silver carp and a preparation method thereof. The compound microbial fish fertilizer special for silver carp comprises the following components in parts by weight: 0.5-0.8 part of silicate bacteria, 1-3 parts of streptococcus faecalis, 0.6-0.8 part of trichoderma sp, 2-5 parts of rhodopseudomonas palustris, 0.3-0.5 part of saccharomycetes, 50-60 parts of fishbone dust, 65-70 parts of vinegar residue powder, 50-55 parts of effluent precipitate, 40-60 parts of rice bran, 12-14 parts of brown sugar, 10-14 parts of table salt, 80-85 parts of pig manure, 45-55 parts of waste of a pig meat slaughter house, 60-65 parts of aquatic plants, 12-16 parts of calcium bicarbonate, 14-17 parts of urea, 1-3 parts of ferric citrate, 0.6-0.8 part of vitamin B1, 0.2-0.6 part of Vitamin B12, 1-3 parts of rheum officinale, 2-6 parts of Astragalus membranaceus and 4-7 parts of medicated leaven. The fertilizer disclosed by the invention can promote silver carp to grow quickly, the EPA content of fish is increased, the fish diseases are reduced, and the number of dead fish is reduced, and meanwhile, beneficial microorganisms in water are increased. Finally, both the quality and the weight of silver carp are improved.
Owner:苏州市阳澄湖现代农业发展有限公司

Environment-friendly biological agent for secondary fermentation of organic fertilizer

The invention relates to the technical field of microbial preparation, and discloses an environment-friendly biological agent for secondary fermentation of an organic fertilizer. The environment-friendly biological agent comprises 400-500 parts of pig manure, 70-110 parts of sweet potato seedlings, 60-100 parts of a nitrogen phosphorus potassium fertilizer, 70-100 parts of soybean meal, 20-40 parts of diatomite, 80-120 parts of stone straw, 10-30 parts of saccharides, 10-20 parts of waste fungus spores, 50-100 parts of turf, 50-80 parts of rice hull powder, 30-50 parts of mushroom residues, 15-25 parts of streptococcus faecalis, 4-7 parts of saccharomycetes, 3-5 parts of cellulase, 5-15 parts of cassava residues, 5-15 parts of sawdust, 6-14 parts of distillers' grains, 3-8 parts of wheat bran, 2-4 parts of tobacco powder and 2-4 parts of furfural residues. The environment-friendly biological agent for secondary fermentation of the organic fertilizer has the advantages of being comprehensive in nutrient and capable of achieving the effects of improving soil, improving soil fertility, increasing yield, enhancing quality and the like; and the selenium content of the soil can be remarkably increased by applying a large amount of the environment-friendly biological agent for the organic fertilizer, and therefore, the absorption performance of the soil is improved.
Owner:上海玖霖环保科技有限公司

A culture medium enriched for Streptococcus faecalis

The invention relates to a culture medium for enriching Streptococcus faecalis. The technical solution adopted is: a culture medium for enriching Streptococcus faecalis, characterized in that it consists of basal medium, salt solution, glucose and yeast extract; in the basal medium, add 3-7ml per 1000ml of basal medium salt solution, 15-20g of glucose and 5-15g of yeast extract; the salt solution is: 40g of MgSO4.7H2O, 2g of MnSO4.4H2O, 2g of FeSO4.7H2O and 2g of NaCl, with distilled water into 1000ml. The culture medium of the invention is used to cultivate the streptococcus faecalis, and the culture speed is fast and the cost is low. The shortcoming of the slow growth period and high cost of the streptococcus faecalis is made up for, the growth rate is increased, and the time for reaching the maximum growth amount is shortened by 3 to 5 hours.
Owner:LIAONING UNIVERSITY

Preparation method of traditional Chinese medicine probiotic fermentation liquor for fish feed

The invention belongs to the technical field of ecological breeding, and discloses a preparation method of traditional Chinese medicine probiotic fermentation liquor for fish feed. The preparation method comprises the following steps: decocting nine traditional Chinese medicines, namely andrographis paniculata, radix isatidis, cyrtomium fortunei, radix astragali seu hedysari, codonopsis pilosula, red dates, pericarpium citri reticulatae, hawthorn and liquorice, and brown sugar in water, diluting a formed decoction, conducting heating to 80-100 DEG C, and adding starch for gelatinization reaction to obtain a suspension fermentation culture solution; and adding composite bacterial powder consisting of bacillus subtilis, saccharomycetes, streptococcus faecalis and rhodospirillum into the suspension fermentation culture solution, conducting stirring, conducting mixing and dispersing uniformly, then adding a nitrogen source and inorganic salt, conducting mixing uniformly, and carrying out suspension fermentation culture under the temperature condition of 10-38 DEG C and the air condition until white to beige fermentation bacterial plaques grow on the surface of the suspension fermentation culture solution, so as to obtain the traditional Chinese medicine probiotic fermentation liquor. The preparation process is simple, and the obtained fermentation liquor is used for feed preparation and can remarkably reduce the feed coefficient.
Owner:梁金玉

Quadruple fluorescent PCR primer set, probe set, kit and method for detecting four pathogenic bacteria in drinking water

ActiveCN108004334BIncrease re-examinationMeet the requirements of pathogenic bacteria detectionMicrobiological testing/measurementDNA/RNA fragmentationBacillus perfringensPseudomonas
The invention discloses a quadruple fluorescent PCR primer set for detecting four pathogenic bacteria in drinking water. The quadruple fluorescent PCR primer set comprises primers for coli groups, streptococcus faecalis, pseudomonas aeruginosa and clostridium perfringens, and is specifically shown as Seq. ID No. 1 to Seq. ID No. 8. The invention belongs to the technical field of gene engineering detection, mutual interference cannot be caused for each primer and each probe, amplification can be carried out on specific objective sequences only, a fluorescence signal is stimulated, non-objectivesequences are free of amplification or fluorescence signal, accurate detection for the four pathogenic bacteria in drinking water can be realized, and the quadruple fluorescent PCR primer set has theadvantages of high specificity, small standard error, short detection time, reagent cost saving and the like and is applicable for detecting the indexes of the pathogenic bacteria in the standard ofdrinking water.
Owner:贵州省产品质量检验检测院

Microbial leaf surface fertilizer with pest preventing effect

The invention relates to a fertilizer, in particular to a microbial leaf surface fertilizer with pest preventing effect. The microbial leaf surface fertilizer with the pest preventing effect is prepared from the following raw materials: urea, magnesium sulfate, zinc sulfate, cotex phellodendri, alkyl glycoside, soybean protein, yeast extract, corn gliadin, ethanol with a volume fraction of 70-80%and streptococcus faecalis powder. The microbial leaf surface fertilizer with the pest preventing effect is directly sprayed on the leaf surface of vegetables, nutrients are directly absorbed from theleaf surface, the growth period is shortened, and the yield is improved; meanwhile, the nutrition of plants is enhanced and the own resistance to disease is improved, a protective film is formed on the leaf surface of the vegetables, insect pests are prevented from being attached to the vegetables, the cotex phellodendri component is contained, and the erosion of germs and insect pests on vegetables can be effectively prevented; and the metabolite produced by streptococcus faecalis destroys the growth environment required for the insect pests and the insect pests are further indirectly inhibited. According to the microbial leaf surface fertilizer with the pest preventing effect, the components of preventing germs and insect pests are plant extracts, are non-polluting to the environment, are more environmentally friendly and healthy, and food safety risks are eliminated.
Owner:崔焰普

Dual fluorescent quantitative PCR (polymerase chain reaction) detection method and kit for two pathogenic bacteria in prepackaged drinking water

The invention discloses a dual fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and kit for two pathogenic bacteria in pre-packaged drinking water. The kit comprises primers and probes (SEQ ID NO: 1-6) for detecting the pseudomonas aeruginosa and the streptococcus faecalis. The kit is good in specificity, the detection method is rapid and simple, the accuracy is high, the sensitivity is good, and a new method is provided for detecting the two pathogenic bacteria of the pseudomonas aeruginosa and the streptococcus faecalis in the pre-packaged drinking water. The dual fluorescent quantitative PCR method provided by the invention can accurately and rapidly realize detection of pseudomonas aeruginosa and streptococcus faecalis in drinking water, can complete detection within one day, has the advantages of rapidness, specificity, sensitivity, high flux, good stability, reagent cost saving and the like, and is suitable for two pathogenic bacteria indexes of pseudomonas aeruginosa and streptococcus faecalis in drinking water standards.
Owner:北京市食品安全监控和风险评估中心(北京市食品检验所)

Pig feed additive containing probiotics and preparation method thereof

The invention discloses a pig feed additive containing probiotics. The pig feed additive containing probiotics includes the following raw materials in parts by weight: 10-18 parts of pine needle powder, 10-15 parts of kelp powder, 5-10 parts of orange peel powder, 5-10 parts of grape seed oil, 1-5 parts of bile acid, 1-5 parts of zinc sulfate, 1-5 parts of milkvetch root, 1-3 parts of fructooligosaccharides, 1-5 parts of lactobacillus acidophilus according to the mass ratio of lyophilized powder, and 1-5 parts of streptococcus faecalis. The pig feed additive provided by the invention uses thenatural forage, the bile acid, the zinc sulfate, the fructooligosaccharides, and two probiotics of the lactobacillus acidophilus and the streptococcus faecalis together, can promote the growth of piglets, enhance the body metabolism, enhance the body immunity, and improve the growth performance of livestock.
Owner:浙江唯娜科技有限公司

Preparation and use method of biochemical agent for biological treatment

The invention discloses a preparation and use method of a biochemical agent for biological treatment, the biochemical agent comprises a plant nutrient, a microbial carrier and fish feed, the plant nutrient comprises cytokinin, gibberellin, furacilin, gentamicin, forchlorfenuron and the like; the microbial carrier is composed of rhodopseudomonas palustris, photosynthetic bacteria, lactic acid bacteria, streptococcus faecalis, clostridium butyricum and the like. The feed can eliminate harmful germs in a water body and inhibit fester and suppuration at the injured part of the body surface of the fish, the quality of the water body is improved while the fish body is treated, high-nutrient substances are provided for the fish by feeding fish feed, and the resistance of the fish body is improved; adjustment from the fish body and the environment is beneficial for accelerating recovery of the fish body, and the pretreatment method for the water body can be suitable for optimizing water quality of a local water area and improving the environment; when the first nutrient substance is prepared, the concentrated mineral substance can also be applied to industries such as food, beverage, medical treatment, health care, beauty, make-up, wine brewing and pet supplies.
Owner:聚宇能科技控股有限公司

Biological preparation for balancing water quality for aquaculture

The invention discloses a biological preparation for balancing water quality for aquaculture. The biological preparation comprises the components of: sodium humate accounting for 49 percent, sodium thiosulfate accounting for 35 percent, biological flocculants accounting for 10 percent, bacillus subtilis accounting for 3 percent, streptococcus faecium accounting for 3 percent, wherein the bacillus subtilis is 3,000,000,000 / g and the streptococcus faecium is 3,000,000,000 / g. According to the biological preparation, the nutrition of a water body is balanced, the water quality is improved, the blue-green algae outburst is controlled, the growth of beneficial algae is activated, dissolved oxygen of the water body is increased, the content of beneficial microorganisms in the water body is increased, the occurrence of chronic nitrate and nitrous acid poison incidents of fishes can be prevented, the immune globulin and the antibody level of the fishes are improved, and the herd immunity of the fishes is improved; and by adding the streptococcus faecium, the true nutritive value of feed is improved, the concentration of ammoniacal nitrogen in excrements also can be reduced, and the pollution to the water quality is reduced.
Owner:JIANGSU HONGJI BIOLOGICAL SCI & TECH

Probiotic honey grapefruit tea and preparation method thereof

InactiveCN108887437AGuaranteed accumulationDoes not affect flavorTea substituesMethanosarcina barkeriStreptococcus fecalis
The invention provides a probiotic honey grapefruit tea and a preparation method thereof. In the technical scheme, lactobacillus casei and streptococcus faecium are taken as probiotic components and are blended with a conventional beverage, so that an intestinal tract probiotic effect is achieved. On such basis, the invention researches an approach for inhibiting growth of two probiotics, in orderto prevent the lactobacillus casei and streptococcus faecium from propagating in the beverage and influencing the flavor of the beverage. An experiment proves that an antagonism effect on propagationefficiency can be achieved when two probiotics are co-cultured with pyrococcus furiosus and methanosarcina barkeri; on such basis, an intergrowth environment and a nutritional condition of four microorganisms are researched; a treating method according to the invention can guarantee the biomass accumulation in early stage and can achieve the balance of flora scale of four microorganisms after mixed cultivation; the blending of the beverage and the mixed bacteria acquired according to the method is capable of keeping bacteria propagation basically stagnated under storage and transportation environments, so that the flavor of the beverage is prevented from being influenced.
Owner:江西韩金实业有限公司
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