Probiotic honey grapefruit tea and preparation method thereof
A technology of honey grapefruit tea and probiotics, which is applied to bacteria, lactobacillus, tea substitutes and other directions used in food preparation, can solve problems such as affecting the natural taste of beverages, and achieve a good promotion prospect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] A probiotic honey grapefruit tea is prepared by the following method:
[0022] 1) Take the Lactobacillus casei strain and inoculate it in 5ml MRS medium, culture it at 37°C for 18 hours, subculture twice to obtain the Lactobacillus casei seed liquid; streak the frozen Streptococcus faecalis on the TSA solid medium, and place it at 37°C Cultivate for 16 hours. After a single colony grows, inoculate it into LB liquid medium, place it on a shaker at 37°C at a speed of 170rpm, and cultivate it for 6 hours. When the OD600 reaches 0.5, the seed liquid of Streptococcus faecalis is obtained; prepare the medium with the following components: K 2 HPO 4 0.4g, NH 4 Cl 1g, MgCl 2 0.1g, potassium oxalate 2.0g, H 3 BO 3 1.5g, L-cysteine-hydrochloride 0.5g, yeast extract 2.0g, vitamin solution 10mL, sodium acetate 10g, 0.2% tris-HCl 1mL, insert Methanosarcina pasteurianus into it, in Cultivate under anaerobic conditions at 22°C until the bacterial cell concentration is 10 6 c...
Embodiment 2
[0049] 1) Take the Lactobacillus casei strain and inoculate it in 5ml MRS medium, culture it at 37°C for 18 hours, subculture twice to obtain the Lactobacillus casei seed liquid; streak the frozen Streptococcus faecalis on the TSA solid medium, and place it at 37°C Cultivate for 16 hours. After a single colony grows, inoculate it into LB liquid medium, place it on a shaker at 37°C at a speed of 170rpm, and cultivate it for 6 hours. When the OD600 reaches 0.5, the seed liquid of Streptococcus faecalis is obtained; prepare the medium with the following components: K 2 HPO 4 0.4g, NH 4 Cl 1g, MgCl 2 0.1g, potassium oxalate 2.0g, H 3 BO 3 1.5g, L-cysteine-hydrochloride 0.5g, yeast extract 2.0g, vitamin solution 10mL, sodium acetate 10g, 0.2% tris-HCl 1mL, insert Methanosarcina pasteurianus into it, in Cultivate under anaerobic conditions at 22°C until the bacterial cell concentration is 10 6 cfu / mL;
[0050] 2) the Lactobacillus casei seed liquid and the Streptococcus fa...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com