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Specific culture medium and quick test slip of streptococcus faecium

A technology of streptococcus faecalis and culture medium, applied in the field of microbiological testing, can solve the problems of inability to quickly evaluate water sanitation, lengthy testing cycle, cumbersome testing procedures, etc., and achieve the effects of improving detection accuracy, convenient observation, and easy operation

Inactive Publication Date: 2013-09-11
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are mainly the following methods for the detection of Streptococcus faecalis in water: one is the traditional method, namely the national standard "Examination Method for Drinking Natural Mineral Water", which mainly uses fermentation method and membrane filtration method; the detection procedure of this method is cumbersome and needs to be verified Test, the detection cycle is long, the workload is heavy, and it is impossible to make a quick evaluation of the water sanitation
The second is to establish a method for detecting Streptococcus faecalis in water by using a filter membrane method based on various biochemical characteristics of Streptococcus faecalis. Target bacteria, the results can be obtained within 24 hours, but the reagents and instruments are more expensive
The third is the rapid detection of chromogenic medium HKCE-microporous membrane and high-pressure vacuum filtration device. This method is still in the research stage, and there is no commercial chromogenic medium

Method used

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  • Specific culture medium and quick test slip of streptococcus faecium
  • Specific culture medium and quick test slip of streptococcus faecium
  • Specific culture medium and quick test slip of streptococcus faecium

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A culture medium for Streptococcus faecalis, containing 30 g of peptone, 10 g of yeast powder, 7 g of sodium chloride, 1.5 g of lactose, 20 g of maltose, 0.2 g of TTC, 0.8 g of nalidixic acid, and 0.07 g of bromocresol purple per 1000 mL of culture medium. Dissolve the above ingredients in purified water to 1000mL, put them in a magnetic stirrer to fully dissolve and mix evenly, and then use.

Embodiment 2

[0029] A culture medium for Streptococcus faecalis, containing 25g of peptone, 20g of yeast powder, 5g of sodium chloride, 1g of lactose, 15g of maltose, 0.15g of TTC, 0.6g of nalidixic acid, and 0.05g of bromocresol purple per 1000mL of culture medium.

[0030] Dissolve the above ingredients in purified water to 1000mL, put them in a magnetic stirrer to fully dissolve and mix evenly, and then use.

Embodiment 3

[0032] A culture medium for Streptococcus faecalis, containing 20 g of peptone, 15 g of yeast powder, 4 g of sodium chloride, 0.8 g of lactose, 10 g of maltose, 0.1 g of TTC, 0.5 g of nalidixic acid, and 0.03 g of bromocresol purple per 1000 mL of culture medium.

[0033] Dissolve the above ingredients in purified water to 1000mL, put them in a magnetic stirrer to fully dissolve and mix evenly, and then use.

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Abstract

The invention discloses a chromogenic medium and a quick test slip of streptococcus faecium. The chromogenic medium of the streptococcus faecium comprises peptone, yeast powder, sodium chloride, lactose, maltose, TTC (Triphenyltetrazolium Chloride), a bacteriostatic agent and an indicator. The quick test slip is a stable microbial culture device comprising a base plate, a water absorbing gel layer, a culture medium layer and a covering film; the chromogenic medium is absorbed on the culture medium layer. The chromogenic medium of the streptococcus faecium is accurate in testing results, obvious in display of positive results, high in specificity, convenient to observe and capable of performing qualitative detection and quantitative analysis to the streptococcus faecium at the same time. According to the quick test slip of the streptococcus faecium, the conventional multi-step detection and identification process is simplified to be done in one step; and in addition, the test slip is small in size and convenient to carry, can sample on site at any time, is simple and convenient to operate, and provides scientific basis and guidance for the water quality safety.

Description

technical field [0001] The invention belongs to the field of microbiological testing, and relates to a specific culture medium for streptococcus faecalis and a quick test piece prepared by using the culture medium. Background technique [0002] Studies in recent years have shown that the diseases caused by streptococcus faecalis have exceeded that of coliform bacteria in various freshwater and marine waters. ISO) and the urban construction standards of the People's Republic of China have added the detection index of Streptococcus faecalis in the water quality monitoring of urban water supply and drinking water. Streptococcus faecalis is an important indicator bacterium of fecal pollution in water bodies, and it can more objectively reflect the sanitation status of water quality. According to the National Inspection Method for Drinking Natural Mineral Water (GB 8538-2008), Streptococcus faecalis is also a mandatory inspection item. [0003] Streptococcus faecalis, also know...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12M1/00
Inventor 蒋廉华卢新
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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