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Composite biological living bacteria preparation and preparation technology thereof

The technology of a live bacteria preparation and a preparation process is applied in the field of new compound biological live bacteria preparation and its preparation, which can solve the problems of hormone residues, urban environment domestic garbage pollution, etc., and achieves promoting growth, improving economic and social benefits, and maintaining ecological balanced effect

Inactive Publication Date: 2015-05-13
角志海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To comprehensively solve the problems of chemistry, hormone residues, urban environmental domestic waste pollution, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0066] Example (mg) 1 2 3 4

[0067] EDTA-2Na 190 195 205 210

[0068] Sodium molybdate 2 hydrate 18 19.5 20.5 22

[0069] Ferrous sulfate heptahydrate 80 90 105 120

[0070] Nickel nitride 6 hydrate 12 11 10 8

[0071] Boric acid 80 90 105 120

[0072] Copper chloride 2 hydrate 80 90 105 120

[0073] Zinc chloride 80 90 105 120

[0074] 4 manganese chloride hydrate 80 90 105 120

[0075] Add 1 liter of distilled water

specific Embodiment

[0076] The above-mentioned multivitamin aqueous solution is also an empirical ratio obtained through repeated trials and formula ratio adjustments to meet the balanced growth requirements of each mixed strain. Specific examples are as follows:

[0077] Examples are as follows:

[0078] Example (g) A B C D

[0079] Vitamin B1 480 490 505 520

[0080] p-Aminobenzoic acid 320 310 300 180

[0081] Vitamin B 3 480 490 505 520

[0082] Vitamin B 12 40 50 55 60

[0083] Vitamin B 6 120 110 95 80

[0084] Vitamin B 40 48 52 60

[0085] Distilled water (L) 1.2 1.1 9.5 8.

[0086] After activating the selected 6 kinds of probiotics separately, and further expanding and cultivating them into liquid strains on a shaking table, the total number of live bacteria reached 10 6 cfu / ml-10 7 cfu / ml. Moreover, the ratio of the number of live bacteria of each strain in the mixed strains shall comply with the percentage composition regula...

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PUM

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Abstract

The invention relates to a composite biological living bacteria preparation and a preparation method thereof. The total number of effective viable bacteria is not less than 108cfu / ml. The method includes: selecting optimal probiotics, carrying out supporting medium activation, performing enlarged culture and mixing, conducting culture by means of a mixed bacteria medium and a fermentation tank, carrying out filtration, concentration and proportioning so as to form the composite living bacteria preparation. The preparation contains the following bacteria with the total number of effective viable bacteria of: 22-25% of photosynthetic bacteria, 21-24% of yeast, 15-20% of bacillus subtilis, 15-20% of Pediococcus acidilactici, 12-16% of Streptococcus faecalis, and 12-16% of Lactobacillus acidophilus. The composite biological living bacteria preparation provided by the invention can be applied to agricultural cultivation, livestock and poultry breeding, aquaculture, feed adding, municipal sewage and domestic garbage treatment and other fields.

Description

technical field [0001] The present invention belongs to the application and production process of microbial strains, specifically a new type of composite biological live bacteria preparation and Its preparation process. Background technique [0002] In the late 1940s, scholars first discovered that tetracycline can promote the growth of livestock and poultry, thus creating the era of antibiotics as feed additives. In this era, people widely use sub-therapeutic doses of antibiotics and other chemicals as feed additives to promote the growth of animals or prevent pathogenic bacteria infections and diseases, making indelible contributions to the development of the feed industry and aquaculture. However, with the deepening of scientific understanding, people have a deeper understanding of the side effects of these antibiotics and chemical drugs while gaining economic benefits. For example, the abuse of antibiotics causes endogenous or superinfection of animals, the emergen...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/125C12R1/01C12R1/46C12R1/23
CPCC12N1/20C12N1/16
Inventor 角志海
Owner 角志海
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