The invention discloses a multiple
fluorescence detection light path applied to a real-time
fluorescence PCR instrument. The multiple
fluorescence detection light path comprises: an emergent light path of a
white light LED
light source, wherein a diaphragm, a collimating lens and a short-pass
optical filter are arranged on the emergent light path of the
white light LED
light source; and a transmission light path of the short-pass
optical filter, wherein a first long-pass dichroic mirror is arranged on the transmission light path of the short-pass
optical filter, a second long-pass two-phase color mirror is arranged on a reflection light path of the first long-pass dichroic mirror, a first receiving collimating lens, a first
narrowband optical filter and a first
silicon photodiode are arranged on the rear side of the
light guide plate, a third long-pass two-phase color mirror is arranged on a reflection light path of the second long-pass two-phase color mirror, the transmission light path is provided with a first objective lens, a second receiving collimating lens, a second narrow-band optical filter and a second
silicon photodiode are arranged on the rear side of the second substrate; a fourth long-pass two-phase color mirror is arranged on a reflection light path of the third long-pass two-phase color mirror; a second objective lens is arranged on the transmission light path,a third receiving collimating lens, a third narrow-band filter and a third
silicon photodiode are arranged on the rear side, a fourth long-pass dichroic
mirror reflection light path is provided with afourth objective lens, a third objective lens is arranged on the transmission light path, a fourth reflection light path is provided with a fourth objective lens, and a fourth receiving collimating lens, a fourth narrow-band filter and a fourth
silicon photodiode are arranged on the rear side.