The invention belongs to the field of insulation detection equipment and relates to a two-manipulator overturning type zero resistance insulator live detection robot. The robot comprises a base and grabbing mechanisms arranged on two sides of the base, wherein each grabbing mechanism comprises a clamping mechanism and an overturning mechanism, the clamping mechanism comprises a first motor and a clamping jaw, the clamping jaw is connected with the first motor through a fixing frame and rotationally connected with the fixing frame, a lead screw nut is arranged at one end, connected with the fixing frame, of the first motor and connected with the clamping jaw through a connecting rod, the connecting position of the connecting rod and the clamping jaw is adjustable, the other end of the firstmotor is connected with one end of a support rod, the other end of the support rod is connected with the overturning mechanism, the overturning mechanism comprises a second motor, the support rod andthe second motor are connected through a rotating shaft, and a limiting block is also arranged between the support rod and the second motor; a detection element is arranged on the base. Conventionalmanual detection rules are broken through, the robot can replace the manual operation procedure in the detection process, the work intensity is reduced and the work efficiency is improved.