Multiple fluorescence detection light path applied to real-time fluorescence PCR instrument

Abstract

The invention discloses a multiple fluorescence detection light path applied to a real-time fluorescence PCR instrument. The multiple fluorescence detection light path comprises: an emergent light path of a white light LED light source, wherein a diaphragm, a collimating lens and a short-pass optical filter are arranged on the emergent light path of the white light LED light source; and a transmission light path of the short-pass optical filter, wherein a first long-pass dichroic mirror is arranged on the transmission light path of the short-pass optical filter, a second long-pass two-phase color mirror is arranged on a reflection light path of the first long-pass dichroic mirror, a first receiving collimating lens, a first narrowband optical filter and a first silicon photodiode are arranged on the rear side of the light guide plate, a third long-pass two-phase color mirror is arranged on a reflection light path of the second long-pass two-phase color mirror, the transmission light path is provided with a first objective lens, a second receiving collimating lens, a second narrow-band optical filter and a second silicon photodiode are arranged on the rear side of the second substrate; a fourth long-pass two-phase color mirror is arranged on a reflection light path of the third long-pass two-phase color mirror; a second objective lens is arranged on the transmission light path,a third receiving collimating lens, a third narrow-band filter and a third silicon photodiode are arranged on the rear side, a fourth long-pass dichroic mirror reflection light path is provided with afourth objective lens, a third objective lens is arranged on the transmission light path, a fourth reflection light path is provided with a fourth objective lens, and a fourth receiving collimating lens, a fourth narrow-band filter and a fourth silicon photodiode are arranged on the rear side.

Description

technical field [0001] The invention relates to a multiple fluorescence detection light path applied to a real-time fluorescence PCR instrument. Background technique [0002] Real-time fluorescent PCR instruments have been widely used due to their good operability, high sensitivity, and high data accuracy and repeatability. The structure is relatively complex, the volume is large, and the adjustment of multiple optical paths is difficult, which restricts the development of miniaturization of multiplex fluorescent PCR instruments. Contents of the invention [0003] Purpose of the invention: Aiming at the problem that the multiple fluorescence detection optical system of the existing real-time fluorescent PCR instrument has a complicated structure and large volume, the present invention provides a coaxial confocal optical system with quadruple fluorescence excitation and detection functions, which can complete different optical path systems in one optical path structure. Ex...

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Problems solved by technology

[0003] Purpose of the invention: Aiming at the problem that the multiple fluorescence detection optical system of the existing real-time fluorescent PCR instrument has a complex structure and large volume, the present invention provides a coaxial confocal optical system with quadruple fluorescence excitation and detection functions, which can complete different optical systems in one optical structure. Excitation and reception of band fluorescence

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