69 results about "Radial chromatography" patented technology

A method for separating and analyzing volatile and semi-volatile flavor components in tobacco by using liquid chromatography-gas chromatography / mass spectrometry technology comprises the following steps: 1. taking a given weight of tobacco products and extracting the volatile and semi-volatile flavor components from the tobacco by simultaneously adopting distillation and extraction; 2. concentrating the extracted samples with a concentrator; 3. carrying out group component separation on the concentrated extracted samples by normal-phase liquid chromatography and collecting distillates by different time sections; and 4. concentrating the collected distillates by the concentrator and respectively entering a gas chromatography-mass spectrometer (GC-MS) for detailed analysis. Compared with theprior art, the method of the invention can effectively extract the volatile and semi-volatile flavor components from the tobacco, carry out group component separation on the extract liquor by normal-phase liquid chromatography, then carry out accurate GC-MS qualitative and quantitative analysis on each group component according to different boiling points, not only has good selectivity and high separation efficiency but also ensures more accurate qualitative and quantitative analysis of each component.

The invention provides an interface device for on-line coupling of LC-GC / MS and a method for analyzing fragrant components in tobaccos by utilizing the device. According to the method, reverse blowing gas can be provided through switch of a gas switch valve, during sample injection, the reverse blowing gas is discharged form a GC flow-splitting port against the GC carrier gas direction through a tee coupling, therefore solvents can be transferred out completely, solutes are reserved in a TenaxTA absorbent in a PTV liner tube, thermal desorption is performed on a PTV injection port after the solvents are transferred completely, the GC carrier gas shifts cut fractions to be tested into a GC column for separation, effects on an MS vacuum system due to the fact the solvents enter the GC column are effectively avoided, and large volume injection can be achieved.

The invention relates to a method for purifying naturally soluble polysaccharides by radial chromatography. After rough polysaccharide solution enters into a radial chromatographic column, the rough polysaccharide solution flows to the center from the periphery of the chromatographic column by flowing through a stationary phase filled by the chromatographic column, corresponding solvent is taken as a mobile phase for elution, and then the solution flows out from a central liquid outlet. As the radial chromatographic column is characterized in that samples have a contact area with filler at the starting position and gather at the liquid outlet, the method can separate or purify the naturally soluble polysaccharides.

The invention discloses a method for measuring trace chloridion and sulfate radical in loprazolam samples by ion chromatography and belongs to the field of analytic chemistry. The method includes the followings steps of preparing and analyzing Cl- and SO42- mixed standard solution and obtaining a linear equation of concentration and peak area, pretreating the loprazolam samples, feeding the treated loprazolam samples to an ion chromatography by a disposable needle type filter, utilizing 3.6mmol / L sodium carbonate as leacheate and analyzing and measuring the content of the chlorodion and the sulfate radical according to the obtained linear equation, wherein a chromatographic column adopted in the ion chromatography is a high-capacity chromatographic column, the inner diameter of filler of the chromatographic column is 5 micrometers, the temperature of a column temperature box is set to be 45 DEG C, sampling input quantity is 100 microliters, applied curb current is 40mA, and flow velocity is 0.8ml / min. The method is quick and simple, high in accuracy, convenient and easy to operate and applicable to quality control in the process of production of loprazolam, the integral analyzing process can be completed within 25min, and the detection requirements of the fine chemical industry are met.

The invention relates to a needle type extraction device suitable for gas chromatography and a using method. The extraction device consists of a heat resistant stainless steel needle head and a section of adsorbents filled inside a needle cylinder, wherein the adsorbents serving as extraction media are used for absorbing a trace amount of volatile or semi-volatile organic compounds; and adsorbent microspheres are fixed inside the needle cylinder by using heat resistant glass cotton. A needle bed of the device can be connected with a simple gas pump or an exchangeable needle head type hermetically sealed injector to extract and concentrate a target material; after the extraction, the device is combined with the gas chromatography to realize thermal desorption under the condition of inert gas serving as a carrier gas and a high temperature of a vaporizing chamber so as to perform the separation and detection in the gas chromatography. The device can also store a sample by sealing the two ends of the device with a Teflon seal head for subsequent analysis when a field test is unavailable. The extraction device can be applied to the analysis of the fast extraction of a trace amount of gas samples in the chemical field.

The invention discloses a detection method and a device for volatile and semi-volatile components. The detection method for the volatile and semi-volatile components comprises the steps of collecting, preparing and analyzing samples and especially comprises the following steps of: performing catching by a filter disc, adding an organic solvent, and performing ultrasonic oscillation to obtain the sample; and performing liquid chromatographic separation on the sample, cutting flow-out components into a catching coil pipe, conveying the sample in the catching coil pipe into a gas chromatography (GC) device, adsorbing the sample by a solid phase extraction column, starting a gas chromatography-mass spectrometer (GC-MS), and analyzing components to be measured in a capillary separation column. The device is a liquid and gas phase bi-dimensional chromatography device, and solvent treatment between the liquid chromatography (LC) device and the gas chromatography device is solid phase extraction. According to the method and the device, the sample can enter a first-level LC with a large size, and fractions of the first-level LC can be cut into a second-level GC with a large size, so that as for the low-content samples, components separated by LC can be accumulated for many times, and then are transferred into the GC for analysis after being accumulated to an ideal analysis amount. Therefore, the samples can be efficiently purified, and the analysis sensitivity is greatly improved.

Systems and methods are provided for preparing samples for chromatographic separations and then chromatographically separating the prepared samples, preferably in a high-throughput fashion utilizing multiple parallel first (fluid) processing regions in fluid communication with multiple parallel second (fluid) processing regions wherein the each second processing region includes a chromatography column. One or more common fluid supplies may be utilized in each of the sample preparation and separation steps to minimize the number of requisite fluid connections and external components such as pumps, reservoirs, pulse dampers, flow controllers, and the like.

The invention relates to a method for detecting four tobacco-specific nitrosamines (TSNAs) in lateral exhaust gas of cigarettes by virtue of gas chromatography-tandem mass spectrometry. The method is characterized by comprising the steps of capturing the lateral exhaust gas of the cigarettes by virtue of a lateral fishtail cover preprocessed by an ascorbic acid solution and a Cambridge filter preprocessed by an ascorbic acid saturated methanol solution, washing the lateral fishtail cover with a hydrochloric acid solution, adding the washed hydrochloric acid solution and an TSNAs internal standard solution into a container in which the Cambridge filter is collected, extracting the Cambridge filter, processing extraction solution by virtue of a solid phase extraction column to obtain an eluate, drying the eluate with nitrogen, redissolving by virtue of dichloromethane to obtain a sample, and analyzing the sample by virtue of the gas chromatography-tandem mass spectrometry (GC-MS / MS). According to the method, the disadvantages of tedious preprocessing process, low detection sensitivity and strong matrix effect in an existing method are overcome, the preprocessing step of the sample is simplified, and the consumption of organic solvents is reduced; the method has the advantages that the detection is accurate, the sensitivity is high, and the repeatability is good.

The invention provides a detection method for simultaneously measuring residual toluene 2,4-diisocyanate (2,4-TDI), toluene 2,6-diisocyanate (2,6-TDI) and diphenylmethane diisocyanate (MDI) in the product by using precolumn derivatization-gas chromatography-mass spectrography. The method comprises the following specific steps: performing ultrasonic extraction on a sample, performing rotary evaporation and concentration on the extracting solution, reacting in an acidic aqueous solution, neutralizing with an alkali liquor, extracting by using an organic solvent, performing organic phase derivatization, removing excessive derivatization reagents with a buffer solution, taking the organic phase as a to-be-measured solution after solution separation, measuring and confirming by using a gas chromatography-mass spectrometer, carrying out a blank experiment, quantifying by using an external standard method, measuring the target compound in the sample solution, and the like. According to the method, the measurement parameters and test steps of the gas chromatography-mass spectrography are reasonably selected, the content of 2,4-TDI, 2,6-TDI and MDI in leather, artificial leather and textiles can be simultaneously measured by adopting selective ion scanning detection and external standard method quantification, and the results are simultaneously obtained by virtue of one-step pretreatment. The method has the characteristics of high precision, high interference resistance and low detection limit.

The invention relates to a method for determining the content of monomer aromatic hydrocarbons in gasoline through gas chromatography to mainly solve problems of large method establishing difficulty and large valve switching system dead volume of present chromatographic methods. The method, which allows an analyte which is the gasoline to be analyzed through a flow switching center cutting chromatographic device, concretely comprises the following steps: injecting the gasoline sample from a chromatographic shunting introduction port (3), pre-separating through a chromatographic column (4), cutting benzene, an internal standard substance and toluene to a chromatographic column (6) through a flow switching center cutting system (10), further separating, and detecting through a hydrogen flame ionization detector (5); and detecting other aromatic hydrocarbon components through allowing them to enter a damping column (2) after they are separated by the chromatographic column (4), wherein the chromatographic column (4) is TCEP[1,2,3-tri(2-cyanethoxy)]propane capillary chromatographic column; and the chromatographic column (6) is a nonpolar chromatographic column. The method well solves the problems through above technical scheme, and can be applied to the industrial production about the chromatographic analysis of gasoline samples.

The invention relates to a molecular engram integral-pin type extraction device suitable for gas chromatography analysis. The device is composed of a high-temperature resistant stainless steel pin and an integral extraction medium in a pin case; the extraction medium is synthesized by one-step reaction according to the molecular engram integral column technology without filling. The device can be connected with a gas collector or an airtight type injector so as to sample, extract and concentrate a specific component in a complex gas sample. The extraction device provided by the invention is designed according to a sample feeding structure of the gas chromatography; the extracted target analyte can be subjected to the thermal desorption with the auxiliary gas as carrier gas under the condition of high temperature in a vaporizing chamber so as to be further separated and detected in the gas chromatography. The device not only has the effects of sampling, extracting and concentrating, but also has the function of storing the sample. When the sample cannot be detected in real time, two ends of the device can be sealed by end caps after sampling, then the sample is stored and the subsequent analysis can be performed as demanded.

The invention discloses a method for simultaneously measuring 28 kinds of pesticide residues with gas chromatography-triple quadrupole tandem mass spectrometry. The method includes the steps of A, sample pretreatment, namely weighing fresh tea leaf powder and pouring the same into a centrifuge tube, adding 1% of an acetonitrile acetic acid solution, adding NaCl after homogenated extraction, and performing full shock blending and centrifugal stratifying prior to taking clear liquid on the upper layer for standby use; B, purification and interior label treatment, namely pouring the clear liquid on the upper layer into the centrifuge tube, purifying the clear liquid with QuEChERS and adding an interior label solution, and taking supernate for standby use after whirlpools shock and centrifugation; C, mass spectrometric measurement, namely subjecting the supernate in the step B to qualitative and quantitative analysis with the gas chromatography-triple quadrupole tandem mass spectrometry. With the method, the problem about measurement blank of the pesticide residues of tea leaves is solved, and the method has the advantages of high flexibility, good selection and high repeatability and accuracy, the 28 kinds of pesticide residues in the green leaves can be measured simultaneously, and the method is specially suitable for measurement analysis for large amount of samples.

A method for separating a mixture of components by simulated moving bed chromatography: (1) feeding the simulated moving bed such that the eluting power of the liquid phase in the second zone is greater than that of the liquid phase in the third zone, trapping the target component inside the second and the third zone; (2) a solvent for dissolving the mixture to be separated, instead of the feeding solution, is fed into the simulated moving bed through the feeding port, and the eluting power of the liquid phase in the second zone is maintained greater than that of the liquid phase in the third zone, thereby both the pre-impurity and the post-impurity are rinsed off completely, and the target component trapped in the second and the third zone is further purified; and (3) the target component trapped in the simulated moving bed is rinsed off the bed.

The invention provides a method for detecting 24 polycyclic aromatic hydrocarbons in edible oil based on a molecularly imprinted polymer solid-phase extraction-gas chromatography / mass spectroscopy hyphenated technique. The method comprises the following steps: (1) preparing a 1.0mg / L standard using liquid, and fixing the volume of an isotope internal standard using liquid to 1.0mg / L; and (2) pre-treatment: accurately weighing 0.50g of edible oil in a 10mL test tube with a plug, firstly, adding 20[mu]l of a mixed internal using liquid, then adding 3mL of normal hexane, whirling and uniformly mixing the mixture for purification, separately activating a high molecularly imprinted polymer solid-phase extraction small column with 5mL of normal hexane and 5mL of dichloromethane, then adding a diluted oil into the small column, separately spraying the small column twice with 6mL of normal hexane and then abandoning the small column, eluting the mixture with a mixed liquid of 10mL of dichloromethane and ethyl acetate in a proportion of 1: 1, collecting the eluent, blowing nitrogen to be nearly dried, adding 0.3mL of normal hexane to dissolve for GC / MS analysis. The method provided by the invention has the beneficial effects of good precision and accuracy and meets the demand of limit valve.

The present disclosure relates to methods for deposition of gas chromatography (GC) stationary phases into chromatography columns, for example gas chromatography columns. A chromatographic medium is dissolved or suspended in a solvent to form a composition. The composition may be inserted into a chromatographic column. Alternatively, portions of the chromatographic column may be exposed or filled with the composition. The composition is permitted to solidify, and at least a portion of the solvent is removed by vacuum sublimation.

The invention discloses a method for detecting chlorobenzene in sludge through ultrasonic assisted dispersive liquid-liquid microextraction-gas chromatography. The method comprises the steps of (1) preparation of a standard solution, namely preparing 0.1milligram / ml of standard mixed solution comprising m-dichlorobenzene, o-dichlorobenzene, 1,2,4-trichlorobenzene, 1,2,3-trichlorobenzene, 1,2,4,5-tetrachlorobenzene, and 1,2,3,4-tetrachlorobenzene; (2) ultrasound-assisted extraction, namely putting 1.0g of a sludge sample into a cone centrifuging tube with a turncap, adding acetone, sealing, extracting by ultrasounds, centrifuging extract liquor, then filtering supernate by a microfiltration membrane, and moving into the cone centrifuging tube; (3) dispersive liquid-liquid microextraction; and (4) gas chromatography. The method for detecting the chlorobenzene compounds in the sludge has the high sensitivity. Compared with conventional detection methods such as a soxhlet extraction method, the method saves a large amount of organic solvent being toxic and harmful to human bodies, and little time is used in analysis, so that the method is applicable to the detection on a large quantity of sludge samples.

The invention relates to the fields of pesticide adjuvant residue detection, drug detection, environmental safety and the like, in particular to a method for detecting residual organic solvents in soil and water by adopting a gas chromatography-mass spectrometry method. The method comprises the following steps that 1, the soil and water to be detected are respectively subjected to solvent extraction and anhydrous Na2SO4 pretreatment and then are centrifuged, supernatant is obtained and filtered to obtain an extraction solution; 2, the gas chromatography-mass spectrometry is adopted to detect the extraction solution in the step 1, wherein a used gas chromatography column is a DB-WAX capillary chromatographic column, and MgSO4 is added in the extraction solution before the chromatographic column is used. Organic solvents include toluene, xylene, DMF and DMSO. The xylene includes dimethylbenzene, paraxylene and dimethylbenzene. The method is fast, convenient and accurate and sensitively detects organic solvent residue quantity in environmental samples such as soil and water.

The invention provides a gel chromatographic column for detecting poly brominated diphenyl ethers and metabolites thereof through a GC-MC (gas chromatography and mass spectrography) method. The gel chromatographic column is prepared by the method comprising the following steps: weighing gel and a solvent, mixing the gel in the solvent, shaking to be uniform and swelling; taking a dry and clean chromatographic column, adding the solvent into the chromatographic column, discharging air bubbles between the lower part of a glass sand core filter plate and a piston, so that the space is filled with the solvent; and pouring the gel suspension liquid into the chromatographic column, and adding sea sand on the gel layer, thereby obtaining the gel chromatographic column. The preparation method of the gel chromatographic column is simple, complicated matrix effect can be overcome, and multiple impurities in the matrix containing organic pollutants can be separated, so that the multiple impurities can be effectively removed, and influences to follow-up detection for the organic pollutants are avoided. The invention further provides a method for detecting poly brominated diphenyl ethers and metabolites thereof based on the gel chromatographic column. The method has the advantages of simplicity in operation, good reproducibility, high sensitivity and high accuracy.

The invention discloses a liquid chromatography-ion chromatography combined system and a determination method; the liquid chromatography-ion chromatography combined system comprises a sample introduction device, a liquid chromatography system, an ion chromatography system, and a ten-way valve. The sample introduction device is connected with the liquid chromatography system and the ion chromatography system through the ten-way valve. The liquid chromatography-ion chromatography combined system realizes combination of ion chromatography and liquid chromatography, solves the problems that inorganic components and organic components cannot be simultaneously quantitatively analyzed at present, and greatly reduces the cost of time; at the same time, with application of an online solid phase extraction column, samples can be directly introduced without any pretreatment, and the operation is more convenient. The method for simultaneous determination of organic compounds and inorganic compounds in water by the liquid chromatography-ion chromatography combined system is simple to operate, and can be used for quantitative analysis of the inorganic components and the organic components in complex water samples at the same time.

chromatography-mass spectrometry, the method comprises the following steps: 1) placing the blasting bead into a solid phase micro-extraction bottle, crushing, covering the solid phase micro-extractionbottle with a solid phase micro-extraction bottle cap, then heating to volatilize and balance components in the blasting bead; aging a solid phase micro-extraction head; 2) inserting the solid phasemicro-extraction head into the solid phase micro-extraction bottle, and adsorbing at 40-120 DEG C for 60-240 min; 3) inserting the adsorbed solid phase micro-extraction head into a sample inlet of a GC-MS instrument, and thermally desorbing at 230-280 DEG C for 1-2 min; and 4) running the GC-MS instrument and collecting data. The method can conveniently, quickly and accurately analyze the volatilecomponents in the blasting bead and avoids the interference and pollution caused by a solvent in the blasting bead. The peak type has no tailing phenomenon, and the components are separated. The components basically cover the common components of the blasting bead, and the method has a good separation and detection effect, and provides an analytical basis for the research and development of a newblasting bead, quality control of blasting bead production, component analysis of blasting bead products, and the like.

The invention discloses a method for measuring dimethyl fumarate in a product by gas chromatography-mass spectrometry. The method comprises the following steps of: pre-treating a dimethyl fumarate product to be detected by using an ultrasonic extraction method; setting experiment conditions of a gas chromatography-mass spectrometer; enabling a sample solution to enter a chromatographic column through a sample inlet, and carrying out temperature programming on the temperature of the chromatographic column to separate the components of the sample solution by the chromatographic column; and enabling the components of the sample solution separated by the chromatographic column to enter a mass spectrometric detection part through a port of the gas chromatography-mass spectrometer. The method for measuring the dimethyl fumarate in the product by the gas chromatography-mass spectrometry has low detection limit, good detection effect, low consumption of an analysis solvent and low detection cost.

The invention discloses a method for separating and purifying C60 by using simulated moving bed chromatography so as to improve the purity and the yield of C60 products. The method comprises the following steps: carbon dust obtained through arc discharge is extracted via toluene to obtain coarse fullerene as the raw material; the methanol / toluene mixed solvent is taken as a mobile phase, the purification is performed through a simulated moving bed, and the purification process includes selection of chromatographic packing material and the mobile phase, preparation of sample solution, preparation of the mobile phase and parameter selection of the simulated moving bed chromatography; then the residual liquid and the liquid extract of the simulated moving bed product are concentrated without liquid respectively through a thin-film rotary vaporator and are dried in a vacuum drying oven to be at constant weight after being eluted with toluene; and finally, the C60 finished product with the purity of 99 percent is obtained through detection by an HPLC method. The invention has high concentration ratio of the separated and purified C60, saves a great amount of mobile phases, can realize the large-scale continuous production and greatly reduces the production cost.

The invention relates to a method for detecting amino acids and saccharides in liquor fermentation liquor through a gas chromatography-mass spectrometry and belongs to the technical field of biochemical detection and measurement. The detection method comprises the steps of (1) taking the fermentation liquor centrifuging at high speed and collecting cells and extracellular fluids respectively; (2) preparing a fermentation liquor sample, namely taking a supernatant liquor obtained in the step 1), adding acetonitrile into the supernatant liquor to remove protein, performing vertex-vibration, then centrifuging for collecting the supernatant liquor III, and adding an internal standard substance ribitol solution to dry at room temperature in vacuum so as to obtain an extracellular fluid sample II; (3) deriving the sample, and adding a saccharide derivating agent and an amino acid derivating agent; (4) performing gas chromatography-mass spectrometry analysis and collecting data. The method for detecting amino acids and saccharides in the fermentation liquor through the gas chromatography-mass spectrometry has the advantages that the method is capable of simply processing the sample, simple and convenient to operate, short in time for detecting amino acids and saccharides, high in sensitivity, high in detection result repeatability and can realize preparation of a plurality of samples.

The invention discloses a method for separating an anti-tumor polysaccharide component from a black fungus by applying radial chromatography. The method comprises the following steps of: 1) extracting the black fungus with hot water; 2) adding Savag agent after concentrating the extracting liquid of the black fungus polysaccharide, shaking and centrifuging; 3) adding alcohol with the volume concentration being more than or equal to 95% into a polysaccharide aqueous solution free from protein, performing alcohol precipitation, and cleaning the obtained precipitation; 4) carrying out freeze drying on coarse black fungus polysaccharide with anti-tumor activity; 5) dissolving the freeze-dried coarse black fungus polysaccharide in distilled water, thereby obtaining a sample solution; pumping the sample solution into a radial chromatograph, selecting DEAE-52 cellulose as padding; respectively eluting by using the distilled water and NaCl solution with the concentration of 0.2M; and 6) collecting the eluent corresponding to the NaCl solution with the concentration of 0.2M, and performing dialysis, concentration and drying in turn, thereby obtaining the black fungus polysaccharide with anti-tumor activity.

The present invention provides a gas chromatography—inverse gas chromatography combined analysis device, which includes a gas chromatography column and an inverse gas chromatography column, an input end of the gas chromatography column is connected to a sample feeder, an output end of the gas chromatography column is connected to an input end of the inverse gas chromatography column, the output end of the gas chromatography column is further connected to a first detector, the input end of the inverse gas chromatography column is further connected to a carrier gas tube, an output end of the inverse gas chromatography column is connected to a second detector, and the first detector and the second detector are both connected to a signal collector. The present invention not only can investigate adsorption performance of a tested solid adsorption material with respect to a single probe, but also can investigate adsorption performance of different solid adsorption materials with respect to different constituents in a combined probe at the same time, thereby improving the development efficiency of the inverse gas chromatography technologies.

The present invention relates to the analysis method of filling cylinder gas chromatography for oxydol working liquid. The method is characterized in that toluene is used as a solution, OV-17 as the fixing liquid for the cylinder of gas chromatography. The gas chromatography quantitative analysis for 2-ethyl-anthraquinone and tetrahydro-2-ethyl antraquinone is performed using filling cylinder made of stainless steel and FID sensor. The method is of high sensitivity and high accuracy and has simple and convenient operation.

A packed column comprises a hollow pipe body and a stationary phase distributed in the pipe body; wherein the stationary phases comprises a stationary phase (A) and a stationary phase (B), both of which are mixed together; the stationary phase (A) is composed of polyethylene glycol and a carrier thereof; the stationary phase (B) is composed of phenyl methyl polysiloxane and the carrier thereof. The invention substitutes the mixed stationary phase packed column comprising polyethylene glycol and phenyl methyl polysiloxane for an organic substance in a capillary tube column quantitative analysis battery electrolyte solution in the prior art, which greatly lowers analysis cost, improves the accuracy of quantitative analysis and guarantees the quality of a battery.

The invention provides an application of a silica-gel chromatographic column to detection of high-purity gas via gas chromatography, and relates to a method and a device for detecting high-purity gas by using the silica-gel chromatographic column. The application of the silica-gel chromatographic column to detection of the high-purity gas has the characteristics that the perfect chromatographic peak shape is provided, high sensitivity and good selectivity are obtained, the sharp peak is realized, the peak capacity is high and the like.

The invention relates to a method for detecting fosfomycin sodium in pharmaceutical wastewater by adopting an ion chromatography. The method comprises the following steps that (1) chromatographic condition: chromatographic columns include a low-volume hydroxyl system cathodic protection column and a high-volume anion exchange separation column, a suppressor is an electrolytic film suppressor, an electrical conductivity detector is used, and leacheate is a sylvite solution; (2) the preparation of a standard solution: a standard product of the fosfomycin sodium is mixed with ultrapure water to obtain into the standard solution; (3) the pretreatment of a sample: a sample to be detected is filtered by using a 0.45mum filtering film and then processed by a C18 colonnette so that organic matters in the water sample are removed; (4) a detecting method: the standard solution is added in an ion chromatograph, then a spectrogram is recorded, the position of a standard peak is determined, the area of a fosfomycin sodium peak is calculated, and a standard curve is drawn according to the calculated area of the peak; a testing sample is added in the ion chromatograph, then a spectrogram is recorded, and the concentration of the sample is calculated according to the area of the fosfomycin sodium peak. The method provided by the invention has the advantages that the detection repeatability of the fosfomycin sodium in the pharmaceutical wastewater is good, the detection limit is low, the sensitivity is high, the operation is simple, the detecting condition is gentle, the detection equipment and instrument are widely applied, the source of a leacheate sylvite solution is wide, and the configuration is simple, and the method is widely used for detecting fosfomycin sodium in the industrial wastewater.

Provided are two-dimensional chromatography systems and methods for separating and / or analyzing complex mixtures of organic compounds. In particularly, a two-dimensional reversed-phase liquid chromatography (RPLC)-supercritical fluid chromatography (SFC) system is described including a trapping column at the interface which collects the analytes eluted from the first dimension chromatography while letting the RPLC mobile phase pass through. The peaks of interest from the RPLC dimension column are effectively focused as sharp concentration pulses on the trapping column, which is subsequently injected onto the second dimension SFC column. The system can be used for simultaneous achiral and chiral analysis of pharmaceutical compounds. The first dimension RPLC separation provides the achiral purity result, and the second dimension SFC separation provides the chiral purity result (enantiomeric excess).