84 results about "Precolumn derivatization" patented technology

The invention discloses a precolumn derivatization and high-efficiency liquid-phase fluorescence detection method for tetrodotoxin. The method includes following steps: (1) preparing a sample solution of a sample to be measured and a standard substance solution of tetrodotoaxin; (2) adding 0.2 ml of a pH maintenance solution to 0.4 ml of the sample solution for controlling pH of the sample solution at a range between 11.6 and 11.9, then adding 0.1 ml of a derivatization agent 1 and 0.1 ml of a derivatization agent 2, mixing uniformly, carrying out a water-bath heating process for 20 min and then carrying out a cooling process to room temperature, adding 0.1 ml of a stopping solution for enabling the pH of the sample solution to be neutral, and then carrying out a then diluting to volume with ultrapure water, adding the standard substance solution of the tetrodotoxin to the sample solution, mixing uniformly, and then carrying out a fluorescence derivatization process to the sample solution; and (3) with a liquid-phase chromatograph instrument equipped with a fluorescence detector, analyzing the sample solution which is subjected to the fluorescence derivatization process in the step (2) and the standard substance solution of the tetrodotoxin to detect the tetrodotoxin qualitatively and quantitatively in the sample solution. The invention also discloses a kit which is suitable for the method. The method is used for detecting the tetrodotoxin in aquatic products qualitatively and quantitatively and is reliable in result and low in detection limit.