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Qualitative and quantitative method for various biogenic amines in white wine

A biogenic amine and liquor technology, applied in the field of liquor analysis and detection, can solve the problem of not establishing the qualitative and quantitative methods of biogenic amines, and achieve the effect of good feasibility and high precision

Active Publication Date: 2013-02-06
JIANGNAN UNIV
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Problems solved by technology

[0002] So far, the research on biogenic amines (BAs) in several flavor-type liquors in China is still blank, and no qualitative and quantitative methods have been established for biogenic amines in various flavor-type liquors in China

Method used

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  • Qualitative and quantitative method for various biogenic amines in white wine
  • Qualitative and quantitative method for various biogenic amines in white wine
  • Qualitative and quantitative method for various biogenic amines in white wine

Examples

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Embodiment 1

[0022] Example 1 Qualitative and quantitative analysis of biogenic amines in a certain commercially available liquor

[0023] After 375mL wine sample was acidified with concentrated hydrochloric acid (pH1.5), use a rotary evaporator (40°C, vacuum) to rotary evaporate to 50mL, and the remaining liquid was extracted twice with dichloromethane, and the raffinate was then rotary evaporated for 2~3 min. Adjust alkaline (pH11), and extract three times with 60mL butanol. The solvent layer was washed with an equal volume of 1M NaOH and then with twice the volume of 1M HCl. The HCl solution was extracted once more with butanol. The remaining hydrochloric acid solution was concentrated to dryness under reduced pressure. Dry salts were derivatized with 5 mL of trifluoroacetic anhydride (TFAA), followed by 20 mL of saturated NaHCO 3 wash. Add solid NaHCO 3 until the solution becomes alkaline. It was then extracted with 15 mL of ether. Diethyl ether solution with 20mL saturated NaHC...

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Abstract

The invention discloses a qualitative and quantitative method for various biogenic amines in white wine and belongs to the technical field of white wine analysis and detection. According to the qualitative and quantitative method, the liquid-liquid extraction is utilized to enrich the biogenic amines in the white wine; derivation is required by both gas phase qualitative treatment and liquid phase quantitative treatment; a derivation agent adopted by the gas phase is TFAA (Trifluoroacetic Anhydride); and dansyl chloride precolumn derivatization is taken as the derivation agent for the liquid phase. According to the method provided by the invention, 9 biogenic amines are firstly determined in the white wine; the limit of detection of the quantitative method can be reduced to about 10ug / L; the linearly dependent coefficient is more than 0.99; the recovery rate is 80.19%-104.05%; the relative standard deviation is within 4%; the biogenic amines in the white wine can be detected by using the qualitative and quantitative method; the feasibility is excellent; and the precision is high.

Description

technical field [0001] Qualitative and quantitative methods for various biogenic amines in liquor, specifically a qualitative and quantitative method for biogenic amines (methylamine, ethylamine, pyrrolidine, putrescine, cadaverine) in liquor, specifically involving the application of gas chromatography- Tandem mass spectrometry (GC-MS / MS) qualitative and UV detection-reversed-phase high-performance liquid chromatography (RP-HPLC) quantitative methods. The invention belongs to the technical field of liquor analysis and detection. Background technique [0002] So far, the research on biogenic amines (BAs) in several flavor-type liquors in China is still blank, and qualitative and quantitative methods for biogenic amines in various flavor-type liquors in China have not been established. The types of biogenic amines present and their amounts are unknown. [0003] Biogenic amines are toxic. When humans and animals ingest excessive amounts of food containing biogenic amines, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/88G01N30/89
Inventor 范文来徐岩温永柱
Owner JIANGNAN UNIV
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