Method for determining monosaccharide constitution of Yupingfeng polysaccharide

Abstract

The invention discloses a method for determining the monosaccharide constitution of Yupingfeng polysaccharide. The method comprises the following steps: (1) preparing a Yupingfeng polysaccharide hydrolysis sample; (2) preparing a mixed monosaccharide standard product; (3) carrying out pre-column derivatization: respectively mixing the Yupingfeng polysaccharide hydrolysis sample obtained in the step (1) and the mixed monosaccharide standard product obtained in the step (2) with a 0.5mol / L PMP methanol solution and a 0.3mol / L NaOH solution, adequately shaking, reacting for 30 minutes under a 68DEG C water bath condition, cooling to the room temperature, adding 0.3mol / L hydrochloric acid for neutralization, adding chloroform, carrying out extraction and centrifugation, abandoning an organiclayer, repeatedly carrying out extraction for 2-3 times to obtain an upper-layer liquid, and filtering by virtue of a 0.22-micron micro-hole filtration film, so as to respectively obtain a Yupingfengpolysaccharide derivatization treatment solution and a mixed monosaccharide derivatization treatment solution for later use; and (4) carrying out high performance liquid chromatography analysis: determining the monosaccharide constitution and content of Yupingfeng polysaccharide. The method is high in sensitivity, small in sample dosage and good in stability, and the monosaccharide constitution ofYupingfeng polysaccharide can be determined through simple operation and relatively low cost.

Description

technical field [0001] The invention relates to the field of determination methods, in particular to the determination of monosaccharide composition of polysaccharides. Background technique [0002] Traditional Chinese medicine polysaccharides have a wide range of biological activities such as enhancing immunity, anti-stress, and anti-virus. They have been widely used in the field of animal husbandry and veterinary medicine, and are also a hot spot in the research of new veterinary drugs. Yupingfeng San comes from "Danxi Xinfa" written by Zhu Danxi in the Yuan Dynasty. It is a representative prescription for strengthening the body in traditional Chinese medicine. It shows that Yupingfeng Powder and its polysaccharides have a wide range of regulatory effects on the immune system, and have a good application prospect in veterinary clinics. [0003] At present, the quality control standards for veterinary traditional Chinese medicine polysaccharides are not perfect, and only t...

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Problems solved by technology

[0004] The purpose of the present invention is to address the deficiencies in the prior art above, to provide a method for determining the composition of monosaccharides in Yupingfeng composite polysaccharides, which solves the problem that polysaccharides and monosaccharides do not have ultraviolet absorption and cannot be directly measured, and The determination method is simple, efficient, and has high precision. It provides important basic data for the creation of new veterinary preparations of Yupingfeng compound polysaccharides, and also provides reference and reference for the study of polysaccharide quality control methods.

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